POCR_SALTY
ID POCR_SALTY Reviewed; 303 AA.
AC Q05587;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Regulatory protein PocR;
GN Name=pocR; OrderedLocusNames=STM2036;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=LT2;
RX PubMed=8071226; DOI=10.1128/jb.176.17.5474-5482.1994;
RA Chen P., Anderson D.I., Roth J.R.;
RT "The control region of the pdu/cob regulon in Salmonella typhimurium.";
RL J. Bacteriol. 176:5474-5482(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=9352910; DOI=10.1128/jb.179.21.6633-6639.1997;
RA Bobik T.A., Xu Y., Jeter R.M., Otto K.E., Roth J.R.;
RT "Propanediol utilization genes (pdu) of Salmonella typhimurium: three genes
RT for the propanediol dehydratase.";
RL J. Bacteriol. 179:6633-6639(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-303.
RC STRAIN=LT2;
RX PubMed=8501034; DOI=10.1128/jb.175.11.3303-3316.1993;
RA Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M.;
RT "Characterization of the cobalamin (vitamin B12) biosynthetic genes of
RT Salmonella typhimurium.";
RL J. Bacteriol. 175:3303-3316(1993).
RN [5]
RP FUNCTION, PATHWAY, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=LT2;
RX PubMed=7559322; DOI=10.1128/jb.177.19.5401-5410.1995;
RA Chen P., Ailion M., Bobik T., Stormo G., Roth J.;
RT "Five promoters integrate control of the cob/pdu regulon in Salmonella
RT typhimurium.";
RL J. Bacteriol. 177:5401-5410(1995).
RN [6]
RP FUNCTION IN PROPANEDIOL UTILIZATION, PATHWAY, AND DISRUPTION PHENOTYPE.
RC STRAIN=LT2;
RX PubMed=9023178; DOI=10.1128/jb.179.4.1013-1022.1997;
RA Walter D., Ailion M., Roth J.;
RT "Genetic characterization of the pdu operon: use of 1,2-propanediol in
RT Salmonella typhimurium.";
RL J. Bacteriol. 179:1013-1022(1997).
CC -!- FUNCTION: Positive regulatory protein of pdu and cob operons
CC (PubMed:8071226, PubMed:7559322, PubMed:9023178). Positively
CC autoregulates its own expression (PubMed:7559322).
CC {ECO:0000269|PubMed:7559322, ECO:0000269|PubMed:8071226,
CC ECO:0000269|PubMed:9023178}.
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis
CC [regulation].
CC -!- PATHWAY: Polyol metabolism; 1,2-propanediol degradation [regulation].
CC {ECO:0000269|PubMed:7559322, ECO:0000269|PubMed:9023178}.
CC -!- DISRUPTION PHENOTYPE: Increases expression of the entire cob operon
CC about 90-fold irrespective of the growth medium; expression retains its
CC sensitivity to vitamin B12 repression (PubMed:8071226). Cells lacking
CC this gene are defective in aerobic and anaerobic degradation of
CC propanediol (PubMed:9023178, PubMed:7559322).
CC {ECO:0000269|PubMed:7559322, ECO:0000269|PubMed:8071226,
CC ECO:0000269|PubMed:9023178}.
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DR EMBL; AF026270; AAB84109.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL20940.1; -; Genomic_DNA.
DR EMBL; L12006; AAA27251.1; -; Genomic_DNA.
DR RefSeq; NP_460981.1; NC_003197.2.
DR RefSeq; WP_000622326.1; NC_003197.2.
DR AlphaFoldDB; Q05587; -.
DR SMR; Q05587; -.
DR STRING; 99287.STM2036; -.
DR PaxDb; Q05587; -.
DR EnsemblBacteria; AAL20940; AAL20940; STM2036.
DR GeneID; 1253557; -.
DR KEGG; stm:STM2036; -.
DR PATRIC; fig|99287.12.peg.2158; -.
DR HOGENOM; CLU_036605_0_1_6; -.
DR OMA; YEGVTPF; -.
DR PhylomeDB; Q05587; -.
DR BioCyc; SENT99287:STM2036-MON; -.
DR UniPathway; UPA00148; -.
DR UniPathway; UPA00621; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0051144; P:propanediol catabolic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR018771; PocR_dom.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR Pfam; PF12833; HTH_18; 1.
DR Pfam; PF10114; PocR; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 1: Evidence at protein level;
KW Activator; Cobalamin biosynthesis; DNA-binding; Glycerol metabolism;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..303
FT /note="Regulatory protein PocR"
FT /id="PRO_0000194546"
FT DOMAIN 195..293
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 212..233
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 260..283
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ SEQUENCE 303 AA; 34414 MW; 5C01E9335C62855B CRC64;
MISASALNSE LINKIAQDFA QATGLAVVVV NIHGDEISEL FNFTPFCQLM RQHPQHSTRC
RMSDRCGGLE ASKSDQPCIY RCHAGLTDFS IPLVIAGHLV GFVLCGQVRL SNDVELVNIL
NVDDRWQADP ELLNEFRNVP EMDYSRVIAS ADLLKLIVEN CLKKQLNFVV IKDNPQQSEA
NKTTRGPTPH DSKMKKALRY IDAHLSDDLR LEDVASHVYL SPYYFSKLFK KYQGIGFNAW
VNRQRMVSAR ELLCHSDWSI ASIARNLGFS QTSYFCKVFR QTYQVTPQAY RQQINENSHP
PSL
