POD1_ARATH
ID POD1_ARATH Reviewed; 624 AA.
AC F4HVJ3; Q8VZM8; Q9C9W2;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Protein POLLEN DEFECTIVE IN GUIDANCE 1;
GN Name=POD1; OrderedLocusNames=At1g67960; ORFNames=T23K23.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, MUTAGENESIS OF
RP 1-MET--LYS-60; ARG-4; ARG-8; 29-ARG-ARG-30; 603-ARG--ASP-624 AND
RP 614-ARG--ARG-616, INTERACTION WITH CRT3, AND TISSUE SPECIFICITY.
RX PubMed=21954464; DOI=10.1105/tpc.111.088914;
RA Li H.J., Xue Y., Jia D.J., Wang T., Hi D.Q., Liu J., Cui F., Xie Q., Ye D.,
RA Yang W.C.;
RT "POD1 regulates pollen tube guidance in response to micropylar female
RT signaling and acts in early embryo patterning in arabidopsis.";
RL Plant Cell 23:3288-3302(2011).
CC -!- FUNCTION: Probable component of the calreticulin 3 (CRT3) complex,
CC acting probably as a co-chaperone involved in protein retention in the
CC endoplasmic reticulum lumen. Required for micropylar pollen tube
CC guidance. Plays an essential role in cell plate orientation or
CC positioning in early embryo patterning. {ECO:0000269|PubMed:21954464}.
CC -!- SUBUNIT: Interacts with CRT3, but not with CRT1 or CNX.
CC {ECO:0000269|PubMed:21954464}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}. Endoplasmic reticulum lumen
CC {ECO:0000269|PubMed:21954464}. Note=Exclusive ER lumen localization and
CC no membrane localization in PubMed:21954464.
CC -!- TISSUE SPECIFICITY: Expressed in inflorescences, siliques, roots and
CC shoots. Expressed in early embryo, endosperm, mature pollen and pollen
CC tubes, synergide cells and weakly in antipodal cells.
CC {ECO:0000269|PubMed:21954464}.
CC -!- DISRUPTION PHENOTYPE: Defective in micropylar pollen tube guidance
CC leading to zygotic lethality. {ECO:0000269|PubMed:21954464}.
CC -!- SIMILARITY: Belongs to the TAPT1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51995.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC012563; AAG51995.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34729.1; -; Genomic_DNA.
DR EMBL; AY064002; AAL36358.1; -; mRNA.
DR PIR; E96702; E96702.
DR RefSeq; NP_176963.2; NM_105466.3.
DR AlphaFoldDB; F4HVJ3; -.
DR STRING; 3702.AT1G67960.1; -.
DR iPTMnet; F4HVJ3; -.
DR PaxDb; F4HVJ3; -.
DR PRIDE; F4HVJ3; -.
DR ProteomicsDB; 234792; -.
DR EnsemblPlants; AT1G67960.1; AT1G67960.1; AT1G67960.
DR GeneID; 843124; -.
DR Gramene; AT1G67960.1; AT1G67960.1; AT1G67960.
DR KEGG; ath:AT1G67960; -.
DR Araport; AT1G67960; -.
DR TAIR; locus:2200181; AT1G67960.
DR eggNOG; KOG2490; Eukaryota.
DR HOGENOM; CLU_003655_4_1_1; -.
DR InParanoid; F4HVJ3; -.
DR OMA; NMRFWIW; -.
DR OrthoDB; 572763at2759; -.
DR PRO; PR:F4HVJ3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HVJ3; baseline and differential.
DR Genevisible; F4HVJ3; AT.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; IDA:TAIR.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0035437; P:maintenance of protein localization in endoplasmic reticulum; IMP:TAIR.
DR GO; GO:0010183; P:pollen tube guidance; IMP:TAIR.
DR InterPro; IPR008010; Tatp1.
DR PANTHER; PTHR13317; PTHR13317; 1.
DR Pfam; PF05346; DUF747; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..624
FT /note="Protein POLLEN DEFECTIVE IN GUIDANCE 1"
FT /id="PRO_0000416783"
FT TRANSMEM 263..283
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 545..565
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 578..598
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 20..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 1..60
FT /note="Missing: Loss of endoplasmic reticulum
FT localization."
FT /evidence="ECO:0000269|PubMed:21954464"
FT MUTAGEN 4
FT /note="R->A: No effect on endoplasmic reticulum
FT localization; when associated with A-8."
FT /evidence="ECO:0000269|PubMed:21954464"
FT MUTAGEN 8
FT /note="R->A: No effect on endoplasmic reticulum
FT localization; when associated with A-4."
FT /evidence="ECO:0000269|PubMed:21954464"
FT MUTAGEN 29..30
FT /note="RR->AA: No effect on endoplasmic reticulum
FT localization."
FT /evidence="ECO:0000269|PubMed:21954464"
FT MUTAGEN 603..624
FT /note="Missing: Loss of endoplasmic reticulum
FT localization."
FT /evidence="ECO:0000269|PubMed:21954464"
FT MUTAGEN 614..616
FT /note="RRR->AAA: Loss of endoplasmic reticulum
FT localization."
FT /evidence="ECO:0000269|PubMed:21954464"
FT MUTAGEN 614..615
FT /note="RR->AA: No effect on endoplasmic reticulum
FT localization."
FT MUTAGEN 615..616
FT /note="RR->AA: No effect on endoplasmic reticulum
FT localization."
FT CONFLICT 225
FT /note="F -> L (in Ref. 3; AAL36358)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 624 AA; 70405 MW; 4F02898B4DE6F828 CRC64;
MAIRSSGRKL SFEILSQNSS FENDDTSIRR SSSDPITGNV ASESPRDYGK RKRSKKKKKK
VNQVETILEN GDSHSTIITG SSGDFGETTT MFENRLNYYG GGGSGSSGGG CVVTLLDGQT
VHHNGFNFGE LRQRNVNGSV DGSNDERWSD TLSSDKKLYM EETSVELSPS ENPPFQEVQH
QFPRSEINGN VVRRLDTEAS LDWKQLVADD PDFLSAETRS PMKYFMEEIY GGISLRSTTT
PGNDIERERI YDTIFRLPWR CEVLIDTGFF VCVNSFLSLL TVMPIRVLLI FMDAFKNRQF
RRPSASELSD LACFLVLATG TILLGRTDIS LIYHMIRGQS TIKLYVVYNI LEIFDRLCQS
FCGDVFGALF SSAKGLSISP PEKLRFSTWR FVSDLALTMA ASILHSFILL AQAITLSTCI
VAHNNALLAL LVSNNFAEIK SSVFKRFSKD NIHGLVYADS IERFHISAFL VSVLAQNILE
SEGAWFGNFI YNATTVFFCE MMIDIIKHSF LAKFNDIKPI AYSEFLQALC EQTLNIRPED
RKTNLTFVPL APACVVIRVL TPVYAAHLPY SPLPWRMLWM VILFVITYIM LTSLKVLIGM
GLRKHATWYI NRCRRRNSSH LHND
