PODN_MOUSE
ID PODN_MOUSE Reviewed; 611 AA.
AC Q7TQ62; A2A8F3; Q8BM45;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Podocan;
DE Flags: Precursor;
GN Name=Podn;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE, TISSUE
RP SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=FVB/N;
RX PubMed=12796502; DOI=10.1074/jbc.m301299200;
RA Ross M.D., Bruggeman L.A., Hanss B., Sunamoto M., Marras D., Klotman M.E.,
RA Klotman P.E.;
RT "Podocan, a novel small leucine-rich repeat protein expressed in the
RT sclerotic glomerular lesion of experimental HIV-associated nephropathy.";
RL J. Biol. Chem. 278:33248-33255(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Negatively regulates cell proliferation and cell migration,
CC especially in smooth muscle cells. {ECO:0000250}.
CC -!- SUBUNIT: Binds to type I collagen. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000269|PubMed:12796502}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7TQ62-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7TQ62-2; Sequence=VSP_021785;
CC -!- TISSUE SPECIFICITY: Kidney. Expressed in podocytes and likely vascular
CC endothelial cells within the glomerulus. {ECO:0000269|PubMed:12796502}.
CC -!- DEVELOPMENTAL STAGE: Detected at low levels in normal embryonic kidney
CC from embryonic day 14 through birth. Expression increases dramatically
CC within 24 hours following birth and maximal levels coincide with the
CC completion of morphogenesis at 2 weeks of age.
CC {ECO:0000269|PubMed:12796502}.
CC -!- PTM: N-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the small leucine-rich proteoglycan (SLRP)
CC family. SLRP class V subfamily. {ECO:0000305}.
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DR EMBL; AY313606; AAP79897.1; -; mRNA.
DR EMBL; AK034987; BAC28904.1; -; mRNA.
DR EMBL; AL611936; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466527; EDL30756.1; -; Genomic_DNA.
DR EMBL; CH466527; EDL30757.1; -; Genomic_DNA.
DR EMBL; BC094340; AAH94340.1; -; mRNA.
DR CCDS; CCDS18445.1; -. [Q7TQ62-1]
DR RefSeq; NP_001272885.1; NM_001285956.1. [Q7TQ62-1]
DR RefSeq; NP_001272887.1; NM_001285958.1. [Q7TQ62-1]
DR RefSeq; NP_766462.2; NM_172874.3. [Q7TQ62-1]
DR RefSeq; XP_006503155.1; XM_006503092.3. [Q7TQ62-1]
DR RefSeq; XP_011238834.1; XM_011240532.1. [Q7TQ62-2]
DR RefSeq; XP_011238835.1; XM_011240533.1.
DR RefSeq; XP_011238836.1; XM_011240534.1. [Q7TQ62-2]
DR AlphaFoldDB; Q7TQ62; -.
DR SMR; Q7TQ62; -.
DR IntAct; Q7TQ62; 1.
DR STRING; 10090.ENSMUSP00000102320; -.
DR GlyGen; Q7TQ62; 3 sites.
DR PhosphoSitePlus; Q7TQ62; -.
DR CPTAC; non-CPTAC-3608; -.
DR MaxQB; Q7TQ62; -.
DR PaxDb; Q7TQ62; -.
DR PRIDE; Q7TQ62; -.
DR ProteomicsDB; 289358; -. [Q7TQ62-1]
DR ProteomicsDB; 289359; -. [Q7TQ62-2]
DR Antibodypedia; 2151; 122 antibodies from 28 providers.
DR DNASU; 242608; -.
DR Ensembl; ENSMUST00000044248; ENSMUSP00000048962; ENSMUSG00000028600. [Q7TQ62-1]
DR Ensembl; ENSMUST00000106708; ENSMUSP00000102319; ENSMUSG00000028600. [Q7TQ62-1]
DR Ensembl; ENSMUST00000106709; ENSMUSP00000102320; ENSMUSG00000028600. [Q7TQ62-1]
DR GeneID; 242608; -.
DR KEGG; mmu:242608; -.
DR UCSC; uc008uaq.1; mouse. [Q7TQ62-1]
DR CTD; 127435; -.
DR MGI; MGI:2674939; Podn.
DR VEuPathDB; HostDB:ENSMUSG00000028600; -.
DR eggNOG; KOG0619; Eukaryota.
DR GeneTree; ENSGT00940000160740; -.
DR HOGENOM; CLU_000288_186_3_1; -.
DR InParanoid; Q7TQ62; -.
DR OMA; SNQLHAR; -.
DR OrthoDB; 826997at2759; -.
DR PhylomeDB; Q7TQ62; -.
DR TreeFam; TF336377; -.
DR BioGRID-ORCS; 242608; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Podn; mouse.
DR PRO; PR:Q7TQ62; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q7TQ62; protein.
DR Bgee; ENSMUSG00000028600; Expressed in digit and 171 other tissues.
DR ExpressionAtlas; Q7TQ62; baseline and differential.
DR Genevisible; Q7TQ62; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0005518; F:collagen binding; ISO:MGI.
DR GO; GO:0030336; P:negative regulation of cell migration; ISS:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:MGI.
DR Gene3D; 3.80.10.10; -; 5.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR026906; LRR_5.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR000372; LRRNT.
DR Pfam; PF13306; LRR_5; 1.
DR Pfam; PF13855; LRR_8; 4.
DR SMART; SM00369; LRR_TYP; 13.
DR SMART; SM00013; LRRNT; 1.
DR PROSITE; PS51450; LRR; 17.
PE 2: Evidence at transcript level;
KW Alternative splicing; Extracellular matrix; Glycoprotein;
KW Leucine-rich repeat; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..611
FT /note="Podocan"
FT /id="PRO_0000262525"
FT DOMAIN 60..97
FT /note="LRRNT"
FT REPEAT 98..119
FT /note="LRR 1"
FT REPEAT 122..145
FT /note="LRR 2"
FT REPEAT 148..169
FT /note="LRR 3"
FT REPEAT 170..190
FT /note="LRR 4"
FT REPEAT 193..213
FT /note="LRR 5"
FT REPEAT 219..239
FT /note="LRR 6"
FT REPEAT 240..261
FT /note="LRR 7"
FT REPEAT 264..284
FT /note="LRR 8"
FT REPEAT 290..311
FT /note="LRR 9"
FT REPEAT 312..332
FT /note="LRR 10"
FT REPEAT 335..358
FT /note="LRR 11"
FT REPEAT 361..382
FT /note="LRR 12"
FT REPEAT 383..403
FT /note="LRR 13"
FT REPEAT 406..427
FT /note="LRR 14"
FT REPEAT 432..453
FT /note="LRR 15"
FT REPEAT 477..490
FT /note="LRR 16"
FT REPEAT 503..523
FT /note="LRR 17"
FT REPEAT 524..545
FT /note="LRR 18"
FT REPEAT 548..569
FT /note="LRR 19"
FT REPEAT 574..583
FT /note="LRR 20"
FT REGION 585..611
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 592..611
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 215
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 282
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 411
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..212
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021785"
SQ SEQUENCE 611 AA; 68718 MW; 38A62119B372A15D CRC64;
MAGSRGLPLL LLVLQLFLGP VLPVRAPVFG RSDTPTLSPE ENEFVEEENQ PVLVLSSEEP
EPGPATVDCP RDCACSQEGV VDCGGIDLRE FPGDLPEHTN HLSLQNNQLE KIYPEELSRL
QRLETLNLQN NRLTSRGLPE EAFEHLTSLN YLYLANNKLT LAPRFLPNAL ISVDFAANYL
TKIYGLTFGQ KPNLRSVYLH NNKLADAGLP DHMFNGSSNV EILILSSNFL RHVPKHLPPA
LYKLHLKNNK LEKIPPGAFS ELSNLRELYL QNNYLTDEGL DNETFWKLSS LEYLDLSSNN
LSRVPAGLPR SLVLLHLEKN AIQSVEADVL TPIRNLEYLL LHSNQLQAKG IHPLAFQGLK
KLHTVHLYNN ALERVPSGLP RRVRTLMILH NQITGIGRED FATTYFLEEL NLSYNRITSP
QMHRDAFRKL RLLRSLDLSG NRLQTLPPGL PKNVHVLKVK RNELAALARG ALAGMAQLRE
LYLTGNRLRS RALGPRAWVD LAGLQLLDIA GNQLTEVPEG LPPSLEYLYL QNNKISAVPA
NAFDSTPNLK GIFLRFNKLA VGSVVESAFR RLKHLQVLDI EGNFEFGNGS KDKDEEEEEE
EEEEDEEEET R
