PODO_RAT
ID PODO_RAT Reviewed; 383 AA.
AC Q8K4G9; Q4G090; Q80WP2; Q8CJE4; Q920E2;
DT 23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 23-APR-2003, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Podocin;
GN Name=Nphs2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Morita H., Yoshimura A., Makino R., Inui K., Nakao N., Usami T.,
RA Roselli S., Antignac C., Matsuyama M., Ideura T.;
RT "Characterization of a proteinuria susceptible gene, Pur1, locus on rat
RT chromosome 13.";
RL Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=Wistar;
RX PubMed=12506137; DOI=10.1097/01.asn.0000037401.02391.76;
RA Kawachi H., Koike H., Kurihara H., Sakai T., Shimizu F.;
RT "Cloning of rat homologue of podocin: expression in proteinuric States and
RT in developing glomeruli.";
RL J. Am. Soc. Nephrol. 14:46-56(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 74-319 (ISOFORM 2).
RA Kwon T., Kim M.J., Kim D.J.;
RT "Rat NPHS2, partial cDNA sequence.";
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 88-319 (ISOFORM 1).
RA Tsukaguchi H., Pollak M.R.;
RT "NPHS2 mutations in FSGS.";
RL Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INTERACTION WITH DDN.
RX PubMed=17537921; DOI=10.1073/pnas.0700917104;
RA Asanuma K., Campbell K.N., Kim K., Faul C., Mundel P.;
RT "Nuclear relocation of the nephrin and CD2AP-binding protein dendrin
RT promotes apoptosis of podocytes.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:10134-10139(2007).
CC -!- FUNCTION: Plays a role in the regulation of glomerular permeability,
CC acting probably as a linker between the plasma membrane and the
CC cytoskeleton. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with nephrin/NPHS1, KIRRL1 and CD2AP (By
CC similarity). Interacts with DDN. {ECO:0000250,
CC ECO:0000269|PubMed:17537921}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8K4G9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K4G9-2; Sequence=VSP_007234;
CC -!- SIMILARITY: Belongs to the band 7/mec-2 family. {ECO:0000305}.
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DR EMBL; AB091379; BAC22515.1; -; mRNA.
DR EMBL; AY039651; AAK71880.1; -; mRNA.
DR EMBL; BC098649; AAH98649.1; -; mRNA.
DR EMBL; AF400653; AAM90639.1; -; mRNA.
DR EMBL; AF309631; AAL09446.1; -; mRNA.
DR RefSeq; NP_570841.2; NM_130828.2. [Q8K4G9-1]
DR AlphaFoldDB; Q8K4G9; -.
DR SMR; Q8K4G9; -.
DR CORUM; Q8K4G9; -.
DR STRING; 10116.ENSRNOP00000005425; -.
DR iPTMnet; Q8K4G9; -.
DR PhosphoSitePlus; Q8K4G9; -.
DR PaxDb; Q8K4G9; -.
DR PRIDE; Q8K4G9; -.
DR Ensembl; ENSRNOT00000005425; ENSRNOP00000005425; ENSRNOG00000004030. [Q8K4G9-1]
DR Ensembl; ENSRNOT00000094869; ENSRNOP00000088544; ENSRNOG00000004030. [Q8K4G9-2]
DR GeneID; 170672; -.
DR KEGG; rno:170672; -.
DR UCSC; RGD:620461; rat. [Q8K4G9-1]
DR CTD; 7827; -.
DR RGD; 620461; Nphs2.
DR eggNOG; KOG2621; Eukaryota.
DR GeneTree; ENSGT01030000234614; -.
DR HOGENOM; CLU_024949_3_0_1; -.
DR InParanoid; Q8K4G9; -.
DR OMA; SVTCVWG; -.
DR OrthoDB; 1062075at2759; -.
DR PhylomeDB; Q8K4G9; -.
DR TreeFam; TF105750; -.
DR Reactome; R-RNO-373753; Nephrin family interactions.
DR PRO; PR:Q8K4G9; -.
DR Proteomes; UP000002494; Chromosome 13.
DR Bgee; ENSRNOG00000004030; Expressed in kidney and 5 other tissues.
DR GO; GO:0005911; C:cell-cell junction; ISO:RGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031235; C:intrinsic component of the cytoplasmic side of the plasma membrane; ISO:RGD.
DR GO; GO:0045121; C:membrane raft; ISO:RGD.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0032991; C:protein-containing complex; ISO:RGD.
DR GO; GO:0036057; C:slit diaphragm; IEA:Ensembl.
DR GO; GO:0031532; P:actin cytoskeleton reorganization; ISO:RGD.
DR GO; GO:0072249; P:metanephric podocyte development; ISO:RGD.
DR Gene3D; 3.30.479.30; -; 1.
DR InterPro; IPR043202; Band-7_stomatin-like.
DR InterPro; IPR001107; Band_7.
DR InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR InterPro; IPR001972; Stomatin_HflK_HflKC_fam.
DR PANTHER; PTHR10264; PTHR10264; 1.
DR Pfam; PF01145; Band_7; 1.
DR PRINTS; PR00721; STOMATIN.
DR SMART; SM00244; PHB; 1.
DR SUPFAM; SSF117892; SSF117892; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..383
FT /note="Podocin"
FT /id="PRO_0000094037"
FT TOPO_DOM 1..102
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 124..383
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..73
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 355..383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..57
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 101
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT VAR_SEQ 248..292
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_007234"
FT CONFLICT 43
FT /note="A -> V (in Ref. 2; AAK71880)"
FT /evidence="ECO:0000305"
FT CONFLICT 51
FT /note="T -> M (in Ref. 2; AAK71880)"
FT /evidence="ECO:0000305"
FT CONFLICT 78..79
FT /note="TE -> RK (in Ref. 2; AAK71880)"
FT /evidence="ECO:0000305"
FT CONFLICT 177
FT /note="H -> L (in Ref. 4; AAM90639)"
FT /evidence="ECO:0000305"
FT CONFLICT 350
FT /note="L -> F (in Ref. 2; AAK71880)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 383 AA; 42387 MW; 4B4D577A810EC21D CRC64;
MDSRARSSSR KTHGRGSRSS SRDDKKSKAG RGNRGRARPD AGAERQSAGR TGTREEHRAP
AATVVNVDEV RSPGEEGTEV VALLESERPE EGIKPSGLGA CEWLLVLSSL IFIIVTFPFS
IWFCIKVVQE YERVIIFRLG HLLPGRAKGP GLFFFLPCLD TYHKVDLRLQ TLEIPFHEVV
TKDMFIMEID AVCYYRMENA SLLLSSLAHV SKAIQFLVQT TMKRLLAHRS LTEILLERKS
IAQDVKVALD SVTCVWGIKV ERTEIKDVRL PAGLQHSLAV EAEAQRQAKV RVIAAEGEKA
ASESLRMAAE ILSGTPAAVQ LRYLHTLQSL STDKPSTVVL PLPFDMLNLL SSPSNRAQGS
INYPSSPKPV EPLNPKRKDS PML
