POF6_SCHPO
ID POF6_SCHPO Reviewed; 872 AA.
AC O74854;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=F-box protein pof6;
GN Name=pof6; ORFNames=SPCC18.04;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11809834; DOI=10.1091/mbc.01-07-0333;
RA Katayama S., Kitamura K., Lehmann A., Nikaido O., Toda T.;
RT "Fission yeast F-box protein Pof3 is required for genome integrity and
RT telomere function.";
RL Mol. Biol. Cell 13:211-224(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION, INTERACTION WITH SKP1, AND SUBCELLULAR LOCATION.
RX PubMed=12511573; DOI=10.1074/jbc.m211358200;
RA Hermand D., Bamps S., Tafforeau L., Vandenhaute J., Makela T.P.;
RT "Skp1 and the F-box protein Pof6 are essential for cell separation in
RT fission yeast.";
RL J. Biol. Chem. 278:9671-9677(2003).
RN [4]
RP INTERACTION WITH SKP1.
RX PubMed=15147268; DOI=10.1111/j.1356-9597.2004.00730.x;
RA Lehmann A., Katayama S., Harrison C., Dhut S., Kitamura K., McDonald N.,
RA Toda T.;
RT "Molecular interactions of fission yeast Skp1 and its role in the DNA
RT damage checkpoint.";
RL Genes Cells 9:367-382(2004).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [6]
RP INTERACTION WITH SIP1 AND SKP1.
RX PubMed=19243310; DOI=10.1042/bj20081659;
RA Jourdain I., Spielewoy N., Thompson J., Dhut S., Yates J.R., Toda T.;
RT "Identification of a conserved F-box protein 6 interactor essential for
RT endocytosis and cytokinesis in fission yeast.";
RL Biochem. J. 420:169-177(2009).
CC -!- FUNCTION: Together with skp1, essential for septum processing and cell
CC separation. {ECO:0000269|PubMed:12511573}.
CC -!- SUBUNIT: Interacts with skp1. Forms a complex with pof6 and skp1.
CC {ECO:0000269|PubMed:12511573, ECO:0000269|PubMed:15147268,
CC ECO:0000269|PubMed:19243310}.
CC -!- INTERACTION:
CC O74854; Q9Y709: skp1; NbExp=4; IntAct=EBI-1185526, EBI-1172248;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Constantly expressed
CC throughout the cell cycle. Expressed in nucleus except the nucleolus
CC and is localized at cell tips on both sides of the septum in septated
CC cells.
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DR EMBL; CU329672; CAA21418.1; -; Genomic_DNA.
DR PIR; T41147; T41147.
DR RefSeq; NP_588383.1; NM_001023374.2.
DR AlphaFoldDB; O74854; -.
DR SMR; O74854; -.
DR BioGRID; 275890; 4.
DR IntAct; O74854; 1.
DR MINT; O74854; -.
DR STRING; 4896.SPCC18.04.1; -.
DR iPTMnet; O74854; -.
DR MaxQB; O74854; -.
DR PaxDb; O74854; -.
DR PRIDE; O74854; -.
DR EnsemblFungi; SPCC18.04.1; SPCC18.04.1:pep; SPCC18.04.
DR GeneID; 2539324; -.
DR KEGG; spo:SPCC18.04; -.
DR PomBase; SPCC18.04; pof6.
DR VEuPathDB; FungiDB:SPCC18.04; -.
DR eggNOG; KOG3745; Eukaryota.
DR HOGENOM; CLU_003875_0_0_1; -.
DR InParanoid; O74854; -.
DR OMA; WYQVKRD; -.
DR PhylomeDB; O74854; -.
DR PRO; PR:O74854; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0032153; C:cell division site; IDA:PomBase.
DR GO; GO:0030428; C:cell septum; IDA:UniProtKB.
DR GO; GO:0051286; C:cell tip; IDA:PomBase.
DR GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0000145; C:exocyst; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:0000151; C:ubiquitin ligase complex; ISM:PomBase.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; ISM:PomBase.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0006897; P:endocytosis; ISO:PomBase.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0006893; P:Golgi to plasma membrane transport; IBA:GO_Central.
DR GO; GO:0000920; P:septum digestion after cytokinesis; IMP:UniProtKB.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR009976; Sec10-like.
DR PANTHER; PTHR12100; PTHR12100; 1.
DR Pfam; PF00646; F-box; 1.
DR Pfam; PF07393; Sec10; 1.
DR SMART; SM00256; FBOX; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..872
FT /note="F-box protein pof6"
FT /id="PRO_0000119973"
FT DOMAIN 30..75
FT /note="F-box"
FT REGION 101..122
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 872 AA; 99912 MW; 0F92F9824F579D6C CRC64;
MKPSEAREHA IRKIKEYLER RHKQQFKALF GCLTINIYLK IFTLISTPDL CNCRLVCRKF
QQLCDYNSIY VKKLLLMNAW DVTLSRKLYY ESQDGMSQAN MSSNTSKGFH LQSSDKKYAD
SDRPKLSSSS LLSLPDQKIP FPVDYSTVQG KQVVLTVLDC VSQRVEFARL DYIKVWRALA
PIYRNLAYAS NTIDPIAFSA FRTPEEQSKV LKYLIRFGNS YPYGTYRQAM QNAILDMASL
FEQACLDEFE LGLHSRNLDL LRKFSHVLHD FSGPNAYVSM YLAKQTDFVR SFFHFDPYSL
FISNNLEEIH INWNILESVV NDTIKLLESE SKFSEATLPE PELVQVPYAK DILGNSLKDY
VISICEHIGE EETELFLVFI SGFYGLCKKF FSIPNGPALV DTIFQPQIDI FISQELHYFK
TVGWSLVDQW DQKLEEKEDA TECFFYKNVS QNTAKNNFLE TFKNVMLLPV SLFTIPSENN
SASNLAEKAI EQKEEEDPEL SKLDAARFVP ANIYVSKDRL KHLPTTELAA QAAVLDSKLE
GISTMFSLEL ALKIVHLCKV SLARAKVFMG TSVPQDDDIK GLSKDLFVQL LRELGQGHLK
HGFDRAIEHL SSFDPRRDFS SNTVEPVVKF LELINVGDMI QQMMDSFFNE EMSPICVKDD
MFDPAIAEKK KFEQLLDERA AFGLHKGINV LIEHADFLLE TKTPMNLFSD QTIGSITNTI
EPTAAAKNVV QFLGFHMRIL VGRADHEILD VFYKEVGMRL FDSLTRYIKS HKFSVDGGLK
LLSDCNLYYE FIHSLHQSSL LPYFKTLKEI AHLFIIDGKN AEEIGKLATD TSRFSSAFHT
EEVYEILHSR IDWLNIKYEV DKVIHGLACC IM
