POF_DROME
ID POF_DROME Reviewed; 495 AA.
AC Q9W123; Q0E8W2;
DT 22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Protein painting of fourth;
DE AltName: Full=Zeste-interacting protein 16;
DE Short=Zip16;
GN Name=Pof; ORFNames=CG3691;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S;
RX PubMed=11353870; DOI=10.1073/pnas.111581298;
RA Larsson J., Chen J.D., Rasheva V., Rasmuson-Lestander A., Pirrotta V.;
RT "Painting of fourth, a chromosome-specific protein in Drosophila.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:6273-6278(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP PARTIAL NUCLEOTIDE SEQUENCE, AND INTERACTION WITH ZESTE.
RA Chen J.D.;
RL Thesis (1992), Baylor College of Medicine / Houston, United States.
CC -!- FUNCTION: Probable RNA-binding protein that specifically binds to the
CC fourth chromosome and may bind an RNA that spreads the fourth
CC chromosome. May be a reminiscence of X chromosome dosage compensation
CC of ancestral Drosophila species in which the X and the fourth
CC chromosomes are one single chromosome.
CC -!- SUBUNIT: Interacts with Zeste. {ECO:0000269|Ref.5}.
CC -!- INTERACTION:
CC Q9W123; Q32KD2: egg; NbExp=2; IntAct=EBI-155974, EBI-140830;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11353870}. Chromosome
CC {ECO:0000269|PubMed:11353870}. Note=Specifically binds to the fourth
CC chromosome.
CC -!- TISSUE SPECIFICITY: Weakly expressed in embryos. Expression increases
CC during larval and pupal stages. In adults, it is predominantly
CC expressed in males, while it is weakly expressed in females.
CC {ECO:0000269|PubMed:11353870}.
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DR EMBL; AJ295236; CAC16735.1; -; mRNA.
DR EMBL; AE013599; AAF47254.1; -; Genomic_DNA.
DR EMBL; AY052060; AAK93484.1; -; mRNA.
DR RefSeq; NP_001246498.1; NM_001259569.2.
DR RefSeq; NP_523851.1; NM_079127.3.
DR RefSeq; NP_726472.1; NM_166682.3.
DR AlphaFoldDB; Q9W123; -.
DR SMR; Q9W123; -.
DR BioGRID; 63519; 8.
DR DIP; DIP-23412N; -.
DR IntAct; Q9W123; 14.
DR STRING; 7227.FBpp0072234; -.
DR PaxDb; Q9W123; -.
DR PRIDE; Q9W123; -.
DR DNASU; 37947; -.
DR EnsemblMetazoa; FBtr0072327; FBpp0072234; FBgn0035047.
DR EnsemblMetazoa; FBtr0072328; FBpp0072235; FBgn0035047.
DR EnsemblMetazoa; FBtr0306655; FBpp0297610; FBgn0035047.
DR GeneID; 37947; -.
DR KEGG; dme:Dmel_CG3691; -.
DR CTD; 37947; -.
DR FlyBase; FBgn0035047; Pof.
DR VEuPathDB; VectorBase:FBgn0035047; -.
DR eggNOG; ENOG502S366; Eukaryota.
DR GeneTree; ENSGT00940000174726; -.
DR HOGENOM; CLU_042851_0_0_1; -.
DR InParanoid; Q9W123; -.
DR OMA; INKVRFG; -.
DR OrthoDB; 608889at2759; -.
DR PhylomeDB; Q9W123; -.
DR SignaLink; Q9W123; -.
DR BioGRID-ORCS; 37947; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Pof; fly.
DR GenomeRNAi; 37947; -.
DR PRO; PR:Q9W123; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0035047; Expressed in testis and 26 other tissues.
DR ExpressionAtlas; Q9W123; baseline and differential.
DR Genevisible; Q9W123; DM.
DR GO; GO:0030849; C:autosome; IDA:UniProtKB.
DR GO; GO:0005694; C:chromosome; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005700; C:polytene chromosome; IDA:FlyBase.
DR GO; GO:0003682; F:chromatin binding; IDA:FlyBase.
DR GO; GO:0003729; F:mRNA binding; ISS:FlyBase.
DR GO; GO:0003723; F:RNA binding; NAS:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:FlyBase.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Chromosome; Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..495
FT /note="Protein painting of fourth"
FT /id="PRO_0000081730"
FT DOMAIN 215..289
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 332..358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 432..451
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 461..495
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 351..367
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 1..30
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 341..358
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 470..495
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 495 AA; 55103 MW; F880E426EA88766D CRC64;
MDSKRAALES GDGPDAKRLD TTDDQDKEAS GGDGSQVMLA KHVAPYTGHG CTPPMESYLF
EPTPAGSQLL PWKTSVDLDN DAELEKPTSD KKPDTAKLSR RELAKMRREH TLRALALERE
LTNKPGQTPA SEVLLVRFPD PEITAPMLAG LSKDIRDVVL PISVAPRYCL VHLKAGADVE
ATICDINRVR FGTGHLRAEL KPFSDEEQAE FIDPCSLYVG NIPFNMTTSA IKAYFANAMR
VDIGVLKREK RARYAFVRYA SPDQTMEAFK ELVDSPLNSR TLTVRYRRLR KRAGMPMVQC
ATSFQALQSP NGDDDNTDCK VISPPPLESI IISDSDNCSD SSGNGKEDGK RKKKINEQER
EIEKLKRQMA EYGAIIKSLQ FRQNSLEDTF IPDLTPKVEP SVNPTGCLLG SNAVHLMRDI
KKECDYLGIP DPVPATKPTT QAQDDSQKKA KRSCFGRLFT GPFRRGTSAM KTADEYEKDD
RLEELYAQLE RDPDP
