POHS1_ARATH
ID POHS1_ARATH Reviewed; 349 AA.
AC Q45GQ7; F4I5W5; Q5BQ17; Q9SAD1;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Protein POOR HOMOLOGOUS SYNAPSIS 1 {ECO:0000303|PubMed:19918061};
GN Name=PHS1 {ECO:0000303|PubMed:19918061};
GN OrderedLocusNames=At1g10710 {ECO:0000312|Araport:AT1G10710};
GN ORFNames=T16B5.15 {ECO:0000312|EMBL:AAD31342.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RA Xiao Y.-L., Underwood B.A., Moskal W., Redman J., Wang W., Monaghan E.,
RA Wu H.C., Utterback T., Town C.D.;
RT "Reconstruction of cDNA sequences for hypothetical genes in Arabidopsis
RT thaliana from 5' and 3' RACE products.";
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-172; ARG-199; SER-203;
RP THR-223 AND PRO-235, AND DISRUPTION PHENOTYPE.
RX PubMed=19918061; DOI=10.1073/pnas.0906273106;
RA Ronceret A., Doutriaux M.P., Golubovskaya I.N., Pawlowski W.P.;
RT "PHS1 regulates meiotic recombination and homologous chromosome pairing by
RT controlling the transport of RAD50 to the nucleus.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:20121-20126(2009).
CC -!- FUNCTION: Required for accurate chromosome segregation in meiosis.
CC Required for pairing to occur between homologous chromosomes. Acts in
CC early recombination steps and ensures pairing fidelity and proper
CC repair of meiotic DNA double-strand-breaks. Regulates recombination and
CC pairing of homologous chromosomes during meiotic prophase by
CC controlling transport of RAD50 from cytoplasm to the nucleus. May
CC affect pairing of the gene-rich fraction of the genome rather than
CC preventing pairing between repetitive DNA elements.
CC {ECO:0000269|PubMed:19918061}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19918061}.
CC Note=Localizes to the cytoplasm during leptotene, zygotene and
CC pachytene. {ECO:0000269|PubMed:19918061}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Comment=A number of isoforms are produced. According to EST
CC sequences. {ECO:0000305};
CC Name=1;
CC IsoId=Q45GQ7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q45GQ7-2; Sequence=VSP_058884;
CC Name=3;
CC IsoId=Q45GQ7-3; Sequence=VSP_058920;
CC -!- DISRUPTION PHENOTYPE: Sterility. Chromosome paring defects during
CC meiosis. {ECO:0000269|PubMed:19918061}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD31342.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007354; AAD31342.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28631.2; -; Genomic_DNA.
DR EMBL; CP002684; AEE28632.2; -; Genomic_DNA.
DR EMBL; DQ132644; AAZ52674.1; -; mRNA.
DR EMBL; DQ132645; AAZ52675.1; -; mRNA.
DR EMBL; AY924661; AAX23736.1; -; mRNA.
DR PIR; E86240; E86240.
DR RefSeq; NP_001077508.1; NM_001084039.1. [Q45GQ7-1]
DR RefSeq; NP_001318974.1; NM_001331923.1. [Q45GQ7-3]
DR AlphaFoldDB; Q45GQ7; -.
DR STRING; 3702.AT1G10710.1; -.
DR PaxDb; Q45GQ7; -.
DR PRIDE; Q45GQ7; -.
DR EnsemblPlants; AT1G10710.1; AT1G10710.1; AT1G10710. [Q45GQ7-1]
DR EnsemblPlants; AT1G10710.2; AT1G10710.2; AT1G10710. [Q45GQ7-3]
DR GeneID; 837614; -.
DR Gramene; AT1G10710.1; AT1G10710.1; AT1G10710. [Q45GQ7-1]
DR Gramene; AT1G10710.2; AT1G10710.2; AT1G10710. [Q45GQ7-3]
DR KEGG; ath:AT1G10710; -.
DR Araport; AT1G10710; -.
DR TAIR; locus:2019908; AT1G10710.
DR eggNOG; ENOG502RYMD; Eukaryota.
DR HOGENOM; CLU_055908_0_0_1; -.
DR OMA; NFQSNFA; -.
DR OrthoDB; 1377750at2759; -.
DR PRO; PR:Q45GQ7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q45GQ7; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Meiosis; Reference proteome.
FT CHAIN 1..349
FT /note="Protein POOR HOMOLOGOUS SYNAPSIS 1"
FT /id="PRO_0000435706"
FT VAR_SEQ 174..226
FT /note="Missing (in isoform 3)"
FT /id="VSP_058920"
FT VAR_SEQ 345..349
FT /note="GNWIT -> DRCVGSTIGRSSKIYIPLCFHNNCIHPFKDDCWCCLAAGTKKD
FT WCWLEKDFPDAKELCMKTCTRKI (in isoform 2)"
FT /id="VSP_058884"
FT MUTAGEN 172
FT /note="C->Y: No effect on meiosis."
FT /evidence="ECO:0000269|PubMed:19918061"
FT MUTAGEN 199
FT /note="R->K: No effect on meiosis."
FT /evidence="ECO:0000269|PubMed:19918061"
FT MUTAGEN 203
FT /note="S->N: No effect on meiosis."
FT /evidence="ECO:0000269|PubMed:19918061"
FT MUTAGEN 223
FT /note="T->I: No effect on meiosis."
FT /evidence="ECO:0000269|PubMed:19918061"
FT MUTAGEN 235
FT /note="P->L: No effect on meiosis."
FT /evidence="ECO:0000269|PubMed:19918061"
SQ SEQUENCE 349 AA; 39499 MW; 162D1059BCF5186A CRC64;
MAGSLTASNR RRNAEDSSEI YRWTIGFARF VHYPSSPSPH PVLKPLGKRE QYHSPHGTWL
SASSSTVSLH IVDELNRSDV ILSVKLGQKV LEEHYISKLN FTWPQMSCVS GFPSRGSRAI
FVTYMDSANQ IQKFALRFST CDAALEFVEA LKEKIKGLKE ASTQNQKNKT RCDVSFQSDY
NPSDAIIPRA TQKEPNMVRP LNSYVPEMLP RIVYEAQYQK SETRSEVSFQ SDYNPSIEIF
PRATEEEPNM VRFFDSSVPE VLPRPEYEAG QALYPSQSTL NQIPSLPPSF TTLLSGCFPD
STLDAGQTTV KQNPDLKSQI LKYMEDSSFQ DMLQKVERII DEIGGNWIT
