POL1_ARMVN
ID POL1_ARMVN Reviewed; 2284 AA.
AC Q6W8W5;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=RNA1 polyprotein;
DE AltName: Full=P1;
DE Contains:
DE RecName: Full=P1A protein;
DE Short=1A;
DE AltName: Full=Protease cofactor;
DE Contains:
DE RecName: Full=Putative ATP-dependent helicase;
DE EC=3.6.4.-;
DE AltName: Full=1B;
DE AltName: Full=Membrane-binding protein;
DE AltName: Full=NTP-binding protein;
DE Short=NTB;
DE Contains:
DE RecName: Full=Viral genome-linked protein;
DE AltName: Full=1C-VPg;
DE Contains:
DE RecName: Full=Picornain 3C-like protease;
DE Short=3C-like protease;
DE EC=3.4.22.-;
DE AltName: Full=1D-PRO;
DE Contains:
DE RecName: Full=RNA-directed RNA polymerase;
DE EC=2.7.7.48;
DE AltName: Full=1E-POL;
OS Arabis mosaic virus (isolate NW) (ArMV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Picornavirales; Secoviridae; Comovirinae; Nepovirus.
OX NCBI_TaxID=282063;
OH NCBI_TaxID=29760; Vitis vinifera (Grape).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=15098112; DOI=10.1007/s00705-003-0277-4;
RA Wetzel T., Beck A., Wegener U., Krczal G.;
RT "Complete nucleotide sequence of the RNA 1 of a grapevine isolate of Arabis
RT mosaic virus.";
RL Arch. Virol. 149:989-995(2004).
CC -!- FUNCTION: Picornain 3C-like protease is a thiol protease that cleaves
CC the P1 and P2 polyproteins. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC -!- SUBCELLULAR LOCATION: [Viral genome-linked protein]: Host endoplasmic
CC reticulum lumen {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Putative ATP-dependent helicase]: Host
CC endoplasmic reticulum membrane {ECO:0000250}; Single-pass membrane
CC protein {ECO:0000250}.
CC -!- PTM: Specific enzymatic cleavages by picornain 3C-like protease in vivo
CC yield mature proteins. Picornain 3C-like protease is autocatalytically
CC processed (By similarity). {ECO:0000250}.
CC -!- PTM: VPg is uridylylated by the polymerase and is covalently linked to
CC the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-
CC peptide primer for the polymerase (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the nepoviruses RNA1 polyprotein family.
CC {ECO:0000305}.
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DR EMBL; AY303786; AAQ73821.1; -; Genomic_RNA.
DR RefSeq; YP_053925.1; NC_006057.1.
DR SMR; Q6W8W5; -.
DR MEROPS; C03.004; -.
DR GeneID; 2943104; -.
DR KEGG; vg:2943104; -.
DR Proteomes; UP000007441; Genome.
DR GO; GO:0044166; C:host cell endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0018144; P:RNA-protein covalent cross-linking; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR Gene3D; 3.30.70.270; -; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir.
DR InterPro; IPR014759; Helicase_SF3_ssRNA_vir.
DR InterPro; IPR044067; PCV_3C_PRO.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR Pfam; PF00680; RdRP_1; 1.
DR Pfam; PF00910; RNA_helicase; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS51874; PCV_3C_PRO; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
DR PROSITE; PS51218; SF3_HELICASE_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Covalent protein-RNA linkage; Helicase;
KW Host endoplasmic reticulum; Host membrane; Hydrolase; Membrane;
KW Nucleotide-binding; Nucleotidyltransferase; Phosphoprotein; Protease;
KW RNA-binding; RNA-directed RNA polymerase; Thiol protease; Transferase;
KW Transmembrane; Transmembrane helix; Viral RNA replication.
FT CHAIN 1..566
FT /note="P1A protein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000037040"
FT CHAIN 567..1216
FT /note="Putative ATP-dependent helicase"
FT /evidence="ECO:0000255"
FT /id="PRO_0000037041"
FT CHAIN 1217..1240
FT /note="Viral genome-linked protein"
FT /evidence="ECO:0000250"
FT /id="PRO_0000037042"
FT CHAIN 1241..1460
FT /note="Picornain 3C-like protease"
FT /evidence="ECO:0000255"
FT /id="PRO_0000037043"
FT CHAIN 1461..2284
FT /note="RNA-directed RNA polymerase"
FT /evidence="ECO:0000255"
FT /id="PRO_0000037044"
FT TOPO_DOM 567..1172
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1173..1193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1194..1216
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT DOMAIN 750..918
FT /note="SF3 helicase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
FT DOMAIN 1242..1457
FT /note="Peptidase C3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT DOMAIN 1727..1851
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT ACT_SITE 1283
FT /note="For picornain 3C-like protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT ACT_SITE 1327
FT /note="For picornain 3C-like protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT ACT_SITE 1419
FT /note="For picornain 3C-like protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT BINDING 780..787
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
FT MOD_RES 1217
FT /note="O-(5'-phospho-RNA)-serine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 2284 AA; 252149 MW; AFE308312E2E4C12 CRC64;
MWQISEGSQC CCTGKTWSNA EAKEARYVCN CILSCRLVKV EVVPQLPKSR IAPAQDKAER
ITPLCNSNGG AAPTIPKSKR AFEPRTPLIK QRCDVVVRVG PPADLDLVYP ALVQEEVAIP
PTEKVLQPTL KAEVRVPIFC APKRMVAFPK PPTKIASKRD ALQFPAGAVA FNGINFIDAK
GKVVLSEGAK RILKGIRVAK QQRQRTARRS AACKKVRKAR DLALFKRLSE ECTFQDLPGG
FAGEIPAGHA CYRKVAAPTT SFKKEVSKGK KAKKPSTPVL PAQDFSCVDS FDWGEKSSPV
EIEDDWVLIE KPVLQRQAAH SAQGRATEAL TRFAASGGFT VKAHQKVEEL ASSGEAGHLI
AGEFAELCLR SLVYNDAPVL SASIEELITE QDFKDAIELF NIELAELPTD STTCGQFNDW
ASAAKKMAKG VGSIVGDFAR MSGAGVLITF DRCIEYLQKK ALTFCQKVFN ATMAPYLSHL
AEASNIISKI WKKLAEWMES LKGKAGLALE VLAQHAIFAL GAIVVGGVVV LVEKVLVACK
VIPNCGIVLG AFLTLFFASL GLTALECTAE EIFRMHQCCK GAIYSMYSVK EPMNEAEGSS
VTMGVLQGLD NAISALTRVG QSMISFKLGS FSYYAKIAQG FDQLARGKKA IGELTGWLID
LVGGVYSKVS GQESTFFDEL STIVCLDVRS WLLKSKRVRL QVETMAIGDR ITLDTISKPT
GMQGHKILIT AAGVPRKTSA DFTMCIKEEV SKLEEVHQRT ACAGINEGMR QFPFWVYIFG
ASQSGKTTIA NSVIIPSLLE EMNLPKTSVY SRPKTGGFWS GYARQACVKV DDFYAIEQTP
SLASSMIDVV NSEPYPLDMA YLHEKGMSMD SPLVVTTANT VKPPTNAGIT DEASFFNRRA
AVIEVRRKDN THFTPRAYDN CIEVRFLHNK CAYVDSEGIP QGPAVNTPME EGWISPSEAV
ATLKNLLGEH VLAEEEKLLD YRERIGNDHP IYNAAQEFIG NMHYPGQWLT TEQKNTYGIN
EEGFSFLAVD GKMYKYNVLG KLNPCETVPP HPNVIPWLEE KTLSIVHWDA HKHIATGPRN
ALVSCFLQGL VQDQSRVQSV DLMGKDSSPE QQAFFKRLTL SERIYLRLCQ IRIDAVKKEQ
LSSVSRGALD VLRDCMYKSK AKLVENYSLL LTLVAILVLI ATAYSLISTL IGLAGCSSFA
GGMVALNHVS NASIPCSEPR LEEGYIPRNK FVSRISRTRG DGPAQGQGDH EELVTELYYY
FDGVKRLISC CWFKGRSLLL TRHQAMAIPI GNEIQVIYAD GTERKLVWPG RQEDRSCKGY
IEFPDNELVV FEHARLLTMP IKYEKFFVDD PDHQISPNVA VKCCVARLED GIPQFHFWNK
YASARSDVHT IKDEGGSAVY QNKIRRYIIY AHEAKRNDCG AIAVAEIQRT PKVLAMLVSG
IGNVTYSSVI PSYSSSFVRG DVPYVPEDGI KTNGYRKVGY LMAKDAPHVP SKTAFMKVPD
EICFPYPNPK QPAILSAEDE RLIGTVHEGY TPIREGMKKF AEPMHLLDAQ LLDEVAGDMV
HTWFDAGEIL EDVPLSIAIN GDVEEEYFDP IAMDTSEGYP EVLQRKNGEK GKARFFVGEP
GAREFVPGCG PERAYLSLEE ECKTRIPSLV SIETPKDERL KRSKIETPGT RLFSVLPLAY
NLLLRVKFLS FSRLLMKKRS HLPCQVGINP YSREWTDLYH RLAEKSDVGY NCDYKGFDGL
ITEQILAVVA TMINAGFRNP VSNQQRSNLL MAISGRLSIC GSQVYETEAG IPSGCALTVV
INSIFNELLM RYCYKKIVPP IYRECFDRCV VLITYGDDNV FTVSQSIMTS FTGDALKAEM
ANLGVTITDG KDKSLATIPA RPLLELEFLK RGFKKGNGGL IYAPLEKLSI MSSLVYIRSD
GSDMLQKLVD NVNTALVELY LHQDREYSES VRDFYLEKLP PGSYKELTTW YEAQIFHECQ
LSGESGWKPQ GLIEVSHGAS FASFVQQNGT ELERHDICPG LAISGSKYIA REEEILMSLS
SLLPGDINAV KLTLKCGDGI GRLPSKASVL SQRKPGIVMQ LCARAIKEKK TLVIRDERPY
IGGWAMACIC GESFGFSIKD TLALYANLMG PNRKNGLATY FTDFDSPVHV KKIHAITNGE
EGVAMLKDSF AFCEPTTIAA TSCDTRKEMV SHLPTSFPNI VLIGGISYPK EGGEPGALYS
PTDVVMSKKL QGVYVSEAVL KCCLRCPGAA VKTVLQTSSP GSSLSQAHFR SLRRVQSHRC
MRKS
