POL1_GCMV
ID POL1_GCMV Reviewed; 2252 AA.
AC P13025;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=RNA1 polyprotein;
DE AltName: Full=P1;
DE Contains:
DE RecName: Full=P1A protein;
DE Short=1A;
DE AltName: Full=Protease cofactor;
DE Contains:
DE RecName: Full=Putative ATP-dependent helicase;
DE EC=3.6.4.-;
DE AltName: Full=1B;
DE AltName: Full=Membrane-binding protein;
DE AltName: Full=NTP-binding protein;
DE Short=NTB;
DE Contains:
DE RecName: Full=Viral genome-linked protein;
DE AltName: Full=1C-VPg;
DE Contains:
DE RecName: Full=Picornain 3C-like protease;
DE Short=3C-like protease;
DE EC=3.4.22.-;
DE AltName: Full=1D-PRO;
DE Contains:
DE RecName: Full=RNA-directed RNA polymerase;
DE EC=2.7.7.48;
DE AltName: Full=1E-POL;
OS Grapevine chrome mosaic virus (GCMV) (Hungarian grapevine chrome mosaic
OS virus).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Picornavirales; Secoviridae; Comovirinae; Nepovirus.
OX NCBI_TaxID=12273;
OH NCBI_TaxID=4045; Apium graveolens (Celery).
OH NCBI_TaxID=29760; Vitis vinifera (Grape).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2798128; DOI=10.1093/nar/17.19.7795;
RA le Gall O., Candresse T., Brault V., Dunez J.;
RT "Nucleotide sequence of Hungarian grapevine chrome mosaic nepovirus RNA1.";
RL Nucleic Acids Res. 17:7795-7807(1989).
CC -!- FUNCTION: Picornain 3C-like protease is a thiol protease that cleaves
CC the P1 and P2 polyproteins. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC -!- SUBCELLULAR LOCATION: [Viral genome-linked protein]: Host endoplasmic
CC reticulum lumen {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Putative ATP-dependent helicase]: Host
CC endoplasmic reticulum membrane {ECO:0000250}; Single-pass membrane
CC protein {ECO:0000250}.
CC -!- PTM: Specific enzymatic cleavages by picornain 3C-like protease in vivo
CC yield mature proteins. Picornain 3C-like protease is autocatalytically
CC processed (By similarity). {ECO:0000250}.
CC -!- PTM: VPg is uridylylated by the polymerase and is covalently linked to
CC the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-
CC peptide primer for the polymerase (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the nepoviruses RNA1 polyprotein family.
CC {ECO:0000305}.
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DR EMBL; X15346; CAA33405.1; -; Genomic_RNA.
DR PIR; S06188; S06188.
DR RefSeq; NP_619705.1; NC_003622.1.
DR SMR; P13025; -.
DR MEROPS; C03.025; -.
DR PRIDE; P13025; -.
DR GeneID; 956641; -.
DR KEGG; vg:956641; -.
DR Proteomes; UP000008624; Genome.
DR GO; GO:0044166; C:host cell endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0018144; P:RNA-protein covalent cross-linking; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR Gene3D; 3.30.70.270; -; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir.
DR InterPro; IPR014759; Helicase_SF3_ssRNA_vir.
DR InterPro; IPR044067; PCV_3C_PRO.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR Pfam; PF00680; RdRP_1; 1.
DR Pfam; PF00910; RNA_helicase; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS51874; PCV_3C_PRO; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
DR PROSITE; PS51218; SF3_HELICASE_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Covalent protein-RNA linkage; Helicase;
KW Host endoplasmic reticulum; Host membrane; Hydrolase; Membrane;
KW Nucleotide-binding; Nucleotidyltransferase; Protease; RNA-binding;
KW RNA-directed RNA polymerase; Thiol protease; Transferase; Transmembrane;
KW Transmembrane helix; Viral RNA replication.
FT CHAIN 1..564
FT /note="P1A protein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000037070"
FT CHAIN 565..1187
FT /note="Putative ATP-dependent helicase"
FT /evidence="ECO:0000255"
FT /id="PRO_0000037071"
FT CHAIN 1188..1216
FT /note="Viral genome-linked protein"
FT /evidence="ECO:0000250"
FT /id="PRO_0000037072"
FT CHAIN 1217..1426
FT /note="Picornain 3C-like protease"
FT /evidence="ECO:0000255"
FT /id="PRO_0000037073"
FT CHAIN 1427..2252
FT /note="RNA-directed RNA polymerase"
FT /evidence="ECO:0000255"
FT /id="PRO_0000037074"
FT TOPO_DOM 565..1140
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1141..1161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1162..1187
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT DOMAIN 733..899
FT /note="SF3 helicase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
FT DOMAIN 1213..1422
FT /note="Peptidase C3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT DOMAIN 1697..1825
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT ACT_SITE 1256
FT /note="For picornain 3C-like protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT ACT_SITE 1294
FT /note="For picornain 3C-like protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT ACT_SITE 1386
FT /note="For picornain 3C-like protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT BINDING 763..770
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
SQ SEQUENCE 2252 AA; 249867 MW; DDFB0DCF7E94171B CRC64;
MMDSTIILPY AKYNYSLNKY LFYNSNLDID LDSFNFYKKY NNTLSLLKRK FLDLFCIPVS
SSSPPAIQKN GLLSASNPKH LFAFANFVLD CGVNPFLQIW EETLANWPLF QGQSLLACCR
TTAEVRREAA EASAEILRLK QVEREHAFQA EVKSLIAAGA LPACAEGLAR KIWSAGKDQR
KVRVAIRTKL TKANALGAAH RSAVATAQAK AEVLREFEPS PAHIQIAVKA HIFAEKLSRK
YADLTAQVRA RRAAARDLRA KEIYLEIVDL LGAPLLSIPQ QIKIKGKYLR RSVAAEVEVP
HTRNPMAELV PYKGLGAVSA DPRCWVVAQC GKFSATHANL CSEIYSMVIG PWVQLTDFSS
IRQFVMNISF LKDFYPEDAL IASLKEVNTE AQLAVAAIVT QEEVSKMEAN ARSANCRANI
FKRIASRITS AACSVKNAFL DGCELTGKRL SEGVFSVVIG HFREALTTIK FELGVAMELV
EVLIARVKSW FDTLLAKIDH ALASLGKWAC YALGILLGIG LCNLIETIIG GHGMLVSLFC
TGVFATMAIK CAGGWDAAQR EMVAMITTLA QSIFGRRKGL DSTDLNTRSI PLLTNVITAM
TTFGTGLCKF QSSSIIEIGK LAGACHQMRM GKEALKEFAA MIMHYLGRIA DKITGRETIF
FDELSTLVSV DVRGWIRCAQ GAILESYHTD PGCETFQDVI GRLVQEGQKL QVGVNGIPRK
ISADYASLIG QIMKDLLELQ KRMMRCGTVT GRRKEPVWIY LWGPSHCGKS NFMDVLGMAL
CKHFDLPYTV CGRNVKDSFF SGYMGQTIME IDDLSSIKTD PPMEGELINL VSCKDYPLNM
ADVADKPIYF KSQFVISSSN QEDVPAGAGV RDISSYRSRK AALIEVRRKP GVIFDPDNAM
AASQARFKDP MTHMLLNGQN DENSWMEMDD VITECINISA RHRAAQEKLQ NASLRAKASL
DPVTLASQEF LRKEVNSVYL EIPTLEIEKA GISGVRGGRC LYADGILYTL GTEFQLIPHP
VENEGYQKLW AQRMKNMYLP AVTTGRYLNA SSMIVTGFLR SLVNGDCAVL SVDALSTTAT
FTQKRIFESL NLAERVYLRA LQCQIDAYTL DIPENPYSNV CWVKMLKALG QGREFIVSNG
GGILMIAAAI ILVLVCGWGF WKAFVGLFTG SMSLGAALAG CQEAEVKAHS VYSADGGDRG
YRSRNIPINH RYSYARSQAG NGLLPASRLC VAIYGPRGVF ISGMQYKNKC VMMTRHQAQS
LNEGDELSVV FASTGESMMI RFHAYHIREN VGSEVVCWLA PSLPQLPCDL KGLFLEDAEV
ELPSNFKSMG YVLRQDSNAF HYDTLDTYAA VDKTPLVLKG VNGDDLYIHE IPEKIVFHYE
SRNNDCGMLL TCQLSGKMKV VGMLVAGKDK TSWACILPNP HLAELKSQIE YIPEFGEAEE
GYFKVGHVSP KEAPTMPKKT NSVQVPQALR VPCDLPIKEP SIISKNDPRC PANVDPPKAA
MKKKFQQPML DLDQKCLDEI AGDMLETWYD CEDSILSDIP LATAINGIPA GCEDAELENF
VMKTSPGYPY FKNKGLGKGK HPYFEECEDG SLKLKEGSQA AELYENMAQF AKEEVPELVV
IECPKDELLP ARKIKVGPCR LFEIMPLHYN LLLRVKTCAF TAFLQHNRHR LPCQVGTNPY
SREWGHLLNR LRRVKTNEAI NCDYSGFDGL LTPQLVEMMA KMINRLYLRS GESEVMQAQR
LNMIMALCGR YALVGTQVYK VNCGLPSGFA LTVVMNSIFN EILIRYAYKT LAPTPEKNSF
GINVCLLVYG DDNLISVSPA VASWFTGEAI RVTLAEKRIK ITDGSDKDAP TIEAKPFSEL
DFLKRKFYVH PEHGQVWAPL DKSAIFSCLH WLTPQKSKFA LQEKACDYLG EVDVVEELII
NVNVSLVELY LHNDKEEFNR VRSFYIARLP MQVDQFRTWA FCEAFHSAQQ TGMLRHDPAK
ILDSLAGPEF PRFMRCSGEG DKAHFYTPIL GVCGPHYKPK EQDFLVSTLP IKQGEGVHIP
IKIGGGVGGL PTHQWVKNFG RPSQLKNNDG YACYKLLCEQ IEQGKRLVFM SAAPYVAGNA
ALISFGSSRK ILKEQMPLCH YRNSIPESVD GLTGYFDAPL PAATIGKSYF ANGETYAALC
EFKNGEVLDI VGPTNVQILN GAVRQGKVPC LAAHSVGTKF KVSLVCNKTM CPHHHHTGPT
FEQAFRTCWL SKCKTKETQV SPWFGTKFLG IS
