POL1_SDVS5
ID POL1_SDVS5 Reviewed; 2081 AA.
AC Q9WAL8;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=RNA1 polyprotein;
DE AltName: Full=P1;
DE Contains:
DE RecName: Full=Putative helicase;
DE EC=3.6.4.-;
DE AltName: Full=Putative NTP-binding protein;
DE Contains:
DE RecName: Full=Probable picornain 3C-like protease;
DE Short=3C-like protease;
DE EC=3.4.22.-;
DE Contains:
DE RecName: Full=Probable RNA-directed RNA polymerase;
DE EC=2.7.7.48;
OS Satsuma dwarf virus (isolate Satsuma mandarin/Japan/S-58/1977) (SDV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Picornavirales; Secoviridae; Sadwavirus; Satsumavirus.
OX NCBI_TaxID=650481;
OH NCBI_TaxID=55188; Citrus unshiu (Satsuma mandarin) (Citrus nobilis var. unshiu).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=10092021; DOI=10.1099/0022-1317-80-3-793;
RA Iwanami T., Kondo Y., Karasev A.V.;
RT "Nucleotide sequences and taxonomy of satsuma dwarf virus.";
RL J. Gen. Virol. 80:793-797(1999).
CC -!- FUNCTION: Picornain 3C-like protease is a thiol protease that probably
CC cleaves the polyprotein. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
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DR EMBL; AB009958; BAA76746.1; -; Genomic_RNA.
DR RefSeq; NP_620566.1; NC_003785.2.
DR SMR; Q9WAL8; -.
DR GeneID; 993327; -.
DR KEGG; vg:993327; -.
DR Proteomes; UP000000675; Genome.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR Gene3D; 3.30.70.270; -; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir.
DR InterPro; IPR014759; Helicase_SF3_ssRNA_vir.
DR InterPro; IPR044067; PCV_3C_PRO.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR Pfam; PF00680; RdRP_1; 1.
DR Pfam; PF00910; RNA_helicase; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS51874; PCV_3C_PRO; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
DR PROSITE; PS51218; SF3_HELICASE_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA replication; Host membrane; Hydrolase; Membrane;
KW Nucleotide-binding; Nucleotidyltransferase; Protease; Reference proteome;
KW RNA-directed RNA polymerase; Thiol protease; Transferase; Transmembrane;
KW Transmembrane helix; Viral RNA replication.
FT CHAIN 1..2081
FT /note="RNA1 polyprotein"
FT /id="PRO_0000402772"
FT CHAIN ?1..?1174
FT /note="Putative helicase"
FT /evidence="ECO:0000255"
FT /id="PRO_0000402773"
FT CHAIN ?1175..?1400
FT /note="Probable picornain 3C-like protease"
FT /evidence="ECO:0000250"
FT /id="PRO_0000402774"
FT CHAIN ?1401..2081
FT /note="Probable RNA-directed RNA polymerase"
FT /evidence="ECO:0000250"
FT /id="PRO_0000402775"
FT TRANSMEM 481..501
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 515..535
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 714..881
FT /note="SF3 helicase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
FT DOMAIN 1166..1388
FT /note="Peptidase C3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT DOMAIN 1679..1810
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT REGION 877..900
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1210
FT /note="For picornain 3C-like protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT ACT_SITE 1255
FT /note="For picornain 3C-like protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT ACT_SITE 1348
FT /note="For picornain 3C-like protease activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT BINDING 741..748
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
SQ SEQUENCE 2081 AA; 230447 MW; D1B0955C25FAF0A3 CRC64;
MEIYGFSPLS FQDTESWKNS AFAACRGCKT YPIEEVGTDG SVRIRELPVG LVLKGVMAQY
RSILWTQFVA DPATTTVDRK SFDFWLFCRR AEAAQERAFR ARQSKRAAKA AALDAEGLID
RSYKGRTYKV STRGLSWKSV RAAQKAARNA AKNFGFAISA NPFSALSALP SEGKGLSAGE
TPATVAEVAP QCFWAALRSL KREFSETPVE DLPFLGLVLP ALPQRNGELA RTLRAQLRAH
QAARVVASGV SLREIPRRTT TTVKVVVPRV TIPVVTFNGA VLHHPKHPVI VALLKRVTIR
AKPICERRTY VRLVDGPGLF PSPRTPTLDL GFMEKVSELA TMPGDSIEKC IAIKCILSND
YNFKEDADMG DVLLSLQSKY TGGGDTAEHR GFISGALYGA GVCVVTSAWK FLDATLHASQ
IGTQISQILD YIQSGLAWLS SSYASIANFF TKGKVYIMEC LELVKAKLPS YLFSAEVGRY
ILLLLGVFLC LGLVNSLIYS VAPQYSLSFG TVCKISLGAL ALVGLGDLVA YLFNSPGAKL
RAFVNIVCNM CGCKNFFAPS SDGEERSGFS VLSMMGAICA LQSLLPANVS RFSWDCGKWA
QTFKTGFDCH EKFATVCESL SVWLLSKVGL FKSSESQAMQ TILLSSGINT CGWLEKVAAF
HSDVHSSQIC IPDLLLRARQ LIETGDTISD LLSNSTVSLS FLLRERIKLA LRELKEDHTQ
LQLAVDVSVS SECPFVLFFA GDSGVGKSTA MKKFREEVLD KLGYPKTARF YPRNPGEKFW
SGYLRHTAVI YDDFAQIPQG DMTYDEAELI RIVTNAVVTV PMAIAEEKGR TFRSKFVFAC
TNRYCESEDA PLADEMAFRR RRHLYIHVAR KPGVEPGPRG VDNLEFSEMD NRDSNGEPAY
QLNDQGKRVL INQKLTYDQL LQLYFERYQA FKVLEGQLEG SVARAPSSSN YEGCDNWWTL
VGSEAKFEAV YFNGEPVSED DQLTQLGNYK TRYDILRARV ILESIDYADC DFIIKNFSAM
GEGIYHADPE VNATGQRHFS AMSPVTRRLI CSCLKTRRED EIKSHSLLSK FKSLLTVPVD
AWKAAPAWFK CISLLLVAGG VGYALCRAIS GIIGIFRKGP AAASLALFSG GIGAVLRGDS
PDDPREERDN PDVIVTKGRG KAIWAGAEIP EALEALRKSQ VVLMGTGRKG EPVMCSALPI
TSHSVLCTTH EIEAFDPNQN MSLIVNNSIY SFVLIPGAIM YKRYDQPNLV DKVHELVRVD
LPKNKGFSLN ARAQFSEDFY DNGTAMKCWV VPNKNPAVAD IMRKVDCEKS SQIIDIFASE
LSRSSGCEPV RQTQRFIYAD GPALNGHCGR LLCANLAGHW RVIGMCAGEG KNRAGVTKAL
YADIPHEFLR ADNLNAVQRG AELDAAILDR FSIPISECRK ELTPMTTRLG YVVGQYPRAL
RKTSIVPSII HDNLWRKPET EPTILGKIDD RSPFPYDPYA TIGEKFVQEV GPIDLSVGSD
ASLVVANIGS SWKAVGKPQC PTVLTWEVAI NGDAAIPYCE RLPLSTSEGY PDSIQRNFGE
KGKKRFFDLK GENVRVPTPA LMEELEVLER ELQKEEVCLT CINTACAKDE KTAPKKVRVQ
PKTRIFEILP FQINIIIRRY LMFWMQLLMV AHDELPSKVG INVYSESWDR LLGRHTRLAN
HFTGDYSGFD TSTPRVLVYA IIDKINELAD DGEVNQRTRR NIIRFVLNRY LISDGVLYEI
HGGTPSGFAP TVMINSVVNE FYLKWSWIGL LKEAGYANQA TLYAFHEATE ISLYGDDNFV
SVATPVASVY NLTTISNFLG RIGVKLGDGA KTGTIKPFIP LEEVDFLKRQ FVADSGSTAI
LCPLKKISIE ERLFYVRGGQ DEIAALELNI ATALCEAFFH GKEYFSFLEG KIIEAMRKSG
VALSRPLPTM ESVRAWYMSQ RGNTKIRSPS FEGLGTMSGI LNIGLAEARS VGGVACFSGI
EFRGRSDDHL MVIPTYIPGG WRTKQQQTYI SFVRDSEKMA QVIKRVAHFS TVVATDKSMA
YLVAICIAYS RGSISRMEVR CHVQNLKVAE MLLCNQICNF L
