ID   POL2_BAMMA              Reviewed;         893 AA.
AC   Q65329;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Genome polyprotein 2;
DE   Contains:
DE     RecName: Full=Helper component proteinase;
DE              Short=HC-pro;
DE              EC=3.4.22.45;
DE   Contains:
DE     RecName: Full=70 kDa protein;
GN   Name=RNA2;
OS   Barley mild mosaic virus (strain ASL) (BaMMV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Stelpaviricetes;
OC   Patatavirales; Potyviridae; Bymovirus.
OX   NCBI_TaxID=103899;
OH   NCBI_TaxID=4513; Hordeum vulgare (Barley).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   AGRICOLA=IND20480706; DOI=10.1007/BF01876238;
RA   Timpe U., Kuehne T.;
RT   "The complete nucleotide sequence of RNA2 of barley mild mosaic virus
RT   (BaMMV).";
RL   Eur. J. Plant Pathol. 100:233-241(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in
CC         the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the
CC         potyviral polyprotein.; EC=3.4.22.45;
CC   -!- PTM: The viral RNA2 of bymoviruses is expressed as a single polyprotein
CC       which undergoes post-translational proteolytic processing resulting in
CC       the production of at least two individual proteins. The HC-pro cleaves
CC       its C-terminus autocatalytically (Potential). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the bymoviruses polyprotein 2 family.
CC       {ECO:0000305}.
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DR   EMBL; X75933; CAA53537.1; -; Genomic_RNA.
DR   SMR; Q65329; -.
DR   MEROPS; C06.002; -.
DR   PRIDE; Q65329; -.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.120.70; -; 1.
DR   Gene3D; 3.90.70.150; -; 1.
DR   InterPro; IPR001456; HC-pro.
DR   InterPro; IPR031159; HC_PRO_CPD_dom.
DR   InterPro; IPR042308; HC_PRO_CPD_sf.
DR   InterPro; IPR036417; TMV-like_coat_sf.
DR   Pfam; PF00851; Peptidase_C6; 1.
DR   SUPFAM; SSF47195; SSF47195; 1.
DR   PROSITE; PS51744; HC_PRO_CPD; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Thiol protease.
FT   CHAIN           1..229
FT                   /note="Helper component proteinase"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000040558"
FT   CHAIN           230..893
FT                   /note="70 kDa protein"
FT                   /id="PRO_0000040559"
FT   DOMAIN          109..229
FT                   /note="Peptidase C6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
FT   REGION          502..539
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        117
FT                   /note="For helper component proteinase activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
FT   ACT_SITE        189
FT                   /note="For helper component proteinase activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
FT   SITE            229..230
FT                   /note="Cleavage; by autolysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
SQ   SEQUENCE   893 AA;  98329 MW;  D6EB74D9D6CF6407 CRC64;
     MMMNSMIRQG WQQVLRRFSI PTSGDRLIVS NSTDQPIGLF GAFDTSLQTL SQVTNDPEVL
     KQKSNIPTHL DVASVLEASP RSFPWVFLTN SFCTFGGSIH AQNLQAFATA EFKSGFCYMN
     LLIPLSFDII DAHADSFRGF VEQLPDTLGA YPSLSMVLNV MLHAATRFPE IVASPIPTIA
     FDAESLQFHV TDKRGVPGMW NILKACRVYE LLSLAADGIG CEYMLYPVGA APQYSFWKKS
     MDHFTSDRFV EFLAMQDLLA SALEQDYATH DARDALLSAL QNAGYTNVVA RERRFPNGHD
     PSIVWLNLSE APISEKLTEL KRYLLVGHRS DDTADITHNV HQHVFEVLKT MSVQFSKTTN
     AYNRARFEVN HKVIWNAEYG RGPQQNAELE ALVLFLNRQS LEIENILHRT TSPVVVTSWK
     PDVPPAAPEI KEEEPTHAIA TPITEAPSHV TPVEVVNLPP TRSYWAETLV GILTAILGTV
     FAFLTRALIR AKRLRRKSTF PWVTLNSGDD DDDQSGGGGG GPQTPGGQPP VPHTRGTHQS
     RFSVQDIASD TSLLSVDLDE DTLSQYDETF QKIRRALFET SFADILQNSA RWISTLEAMA
     LADGNAPYTL LAQYLNGIEE AYTNFRNTGH ISRATLSGFF ALEDNLRAAG IAFGTTTPTQ
     TIQNQFADSP ARRWKTRFEQ IACELGDASI KSLADLADII DTERERGDLT QFDVLAASSI
     SSLCRAVRII SDTTDPNAQL ALVENATAMQ NNINAILGTN VSIPFLSATR RLLITRRVQE
     AGAESRSGAT PETVQQLADA ELAKIVSEAN MYNEMAASQR DIANATREAT IREHVLSPVN
     ALANVGMAAA FFRSGGLRSR AFNPAMPTMP GGPAAAGRPM FQAFRGRGHR LNR