ID   POL2_BAMMN              Reviewed;         891 AA.
AC   P89684;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Genome polyprotein 2;
DE   Contains:
DE     RecName: Full=Helper component proteinase;
DE              Short=HC-pro;
DE              EC=3.4.22.45;
DE   Contains:
DE     RecName: Full=70 kDa protein;
GN   Name=RNA2;
OS   Barley mild mosaic virus (strain Na1) (BaMMV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Stelpaviricetes;
OC   Patatavirales; Potyviridae; Bymovirus.
OX   NCBI_TaxID=103900;
OH   NCBI_TaxID=4513; Hordeum vulgare (Barley).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8973524; DOI=10.1007/bf01718216;
RA   Kashiwazaki S.;
RT   "The complete nucleotide sequence and genome organization of barley mild
RT   mosaic virus (Na1 strain).";
RL   Arch. Virol. 141:2077-2089(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in
CC         the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the
CC         potyviral polyprotein.; EC=3.4.22.45;
CC   -!- PTM: The viral RNA2 of bymoviruses is expressed as a single polyprotein
CC       which undergoes post-translational proteolytic processing resulting in
CC       the production of at least two individual proteins. The HC-pro cleaves
CC       its C-terminus autocatalytically (Potential). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the bymoviruses polyprotein 2 family.
CC       {ECO:0000305}.
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DR   EMBL; D83409; BAA18954.1; -; Genomic_RNA.
DR   SMR; P89684; -.
DR   MEROPS; C06.002; -.
DR   Proteomes; UP000007444; Genome.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.120.70; -; 1.
DR   Gene3D; 3.90.70.150; -; 1.
DR   InterPro; IPR001456; HC-pro.
DR   InterPro; IPR031159; HC_PRO_CPD_dom.
DR   InterPro; IPR042308; HC_PRO_CPD_sf.
DR   InterPro; IPR036417; TMV-like_coat_sf.
DR   Pfam; PF00851; Peptidase_C6; 1.
DR   SUPFAM; SSF47195; SSF47195; 1.
DR   PROSITE; PS51744; HC_PRO_CPD; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Thiol protease.
FT   CHAIN           1..229
FT                   /note="Helper component proteinase"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000040560"
FT   CHAIN           230..891
FT                   /note="70 kDa protein"
FT                   /id="PRO_0000040561"
FT   DOMAIN          109..229
FT                   /note="Peptidase C6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
FT   REGION          502..540
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        117
FT                   /note="For helper component proteinase activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
FT   ACT_SITE        189
FT                   /note="For helper component proteinase activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
FT   SITE            229..230
FT                   /note="Cleavage; by autolysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
SQ   SEQUENCE   891 AA;  97943 MW;  A290247196822BE2 CRC64;
     MMMNSTIRQG WQQVLKRFSI PASGDRLIIS NPTDQPIGLF GAFDTSLQTL SQVGDDPEVL
     KQKIHIPTHL DIASALEASP RSFPWIFLTN SFCTFGGSIH AQNLQAFATA EFKSGFCYMN
     LLVPLSFDII DAHADSFRVF VEQLPDMLGA YPSLSMVLNV MLHAATRFPE IVSSPVPTIA
     FDAESLQFHV TDKRGVPGMW NILKAGRVYE LLSLAADGVG CEYMLYPVGA APQYSFWKKS
     MDHFTSDRFV EFLAMQNLLA SALEQDYTTH DALDALLAAL QNAGYTNVVA RERRFPNGHD
     PSTVWLNLSE APISEKLTDL KRYLLVGHRS DDTADITHNV HQYVFEVLKT MSVQFSKRTN
     AYNRARFEVN HKVIWNAEYG RGPQQNAELE ALVLFLNRQS LEIENILHRT TSPVVVTNWQ
     PDVPTAAPEV SEGEPTHAVA TPMTEAPAHA TPVEVVNLPS TRSYWAETLV GVLTAVLGTI
     FALLTRALIR AKRLRRKPTF PWVTLDSGDE DDDHSGGGGG GPQTPGGQPP ASPAHRTHQS
     RLSVQDIASD TSLLSVDLDE DTLSQYDETF QRIRRALFET SFTDILQNSA RWISALEAMA
     LADGNAPYTL LAQYLNGIEE AYTSFRNTGH VSRATLSSFF ALEDSLRAAG IAFGATTPTQ
     TIQNQFADSP ARRWKTRFEQ IACELGDASI KSLADLADII DTERERSDLT QFDVLAASSI
     SSLCRAVRII SDTTDPDAQL ALVENATAMQ NNINAILGTN VSIPFLSATR RLLVTRRIQQ
     AGAENRSGAT PETIQQLADA ELIVSEANMF NEMATSQRDI ANATQEATIR EHVLSPVNAL
     ANVGMAAAFF RSGGMRSRAL HPAMPTMPGV SAATGRPIFQ AFRGRGHRLN R