ID   POL2_BAYMG              Reviewed;         890 AA.
AC   Q01365;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Genome polyprotein 2;
DE   Contains:
DE     RecName: Full=Helper component proteinase;
DE              Short=HC-pro;
DE              EC=3.4.22.45;
DE   Contains:
DE     RecName: Full=70 kDa protein;
GN   Name=RNA2;
OS   Barley yellow mosaic virus (isolate Germany) (BaYMV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Stelpaviricetes;
OC   Patatavirales; Potyviridae; Bymovirus.
OX   NCBI_TaxID=31728;
OH   NCBI_TaxID=4513; Hordeum vulgare (Barley).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2016598; DOI=10.1099/0022-1317-72-4-989;
RA   Davidson A.D., Proels M., Schell J., Steinbiss H.H.;
RT   "The nucleotide sequence of RNA 2 of barley yellow mosaic virus.";
RL   J. Gen. Virol. 72:989-993(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in
CC         the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the
CC         potyviral polyprotein.; EC=3.4.22.45;
CC   -!- PTM: The viral RNA2 of bymoviruses is expressed as a single polyprotein
CC       which undergoes post-translational proteolytic processing resulting in
CC       the production of at least two individual proteins. The HC-pro cleaves
CC       its C-terminus autocatalytically (Potential). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the bymoviruses polyprotein 2 family.
CC       {ECO:0000305}.
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DR   EMBL; D01099; BAA00884.1; -; Genomic_RNA.
DR   PIR; JQ1947; JQ1947.
DR   SMR; Q01365; -.
DR   MEROPS; C06.002; -.
DR   Proteomes; UP000007446; Genome.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.120.70; -; 1.
DR   Gene3D; 3.90.70.150; -; 1.
DR   InterPro; IPR001456; HC-pro.
DR   InterPro; IPR031159; HC_PRO_CPD_dom.
DR   InterPro; IPR042308; HC_PRO_CPD_sf.
DR   InterPro; IPR036417; TMV-like_coat_sf.
DR   Pfam; PF00851; Peptidase_C6; 1.
DR   SUPFAM; SSF47195; SSF47195; 1.
DR   PROSITE; PS51744; HC_PRO_CPD; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Thiol protease.
FT   CHAIN           1..255
FT                   /note="Helper component proteinase"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000040554"
FT   CHAIN           256..890
FT                   /note="70 kDa protein"
FT                   /id="PRO_0000040555"
FT   DOMAIN          135..255
FT                   /note="Peptidase C6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
FT   REGION          508..533
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        515..529
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        143
FT                   /note="For helper component proteinase activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
FT   ACT_SITE        215
FT                   /note="For helper component proteinase activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
FT   SITE            255..256
FT                   /note="Cleavage; by autolysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
SQ   SEQUENCE   890 AA;  98204 MW;  753AB79EABAD3640 CRC64;
     MSTSSSRLFF DCGSLDWPNK SLFGDPTTRD VMDEHISSTW NAVIRRHMLA PNADAETILG
     RDGLPSAQFD AYGAMLPSFI QALNAPTTRL RISAPLSTAE SILCADASHA PWLYMANSVC
     AYEATHLQPV QTFIAFNFAH GYCYLSLFIP LSFRITPENA RSFSRFLEQL PDILGAYPTL
     ASLYKTMLFA VRLFPEVLQA PIPIIAKRPG VLQFHVSDAR GLPPSWFPMK CGSVASFIAL
     ITNNLNSDLL NGIVGSNGDG EHYTNWNSGH NHWIVNRFIT VKDLHSSLKS ALEVDLDTEG
     GRNAVLDLLL DLGVTNLVRR EKRFPAYFQG AESVYLLLSC ERVGNELVAV QDALQEPLAN
     YTGKDLRALI INLGGLPSRH PEICYTRNIF ENDNHLVWNF EFYRIASITK NAQIDRDVLS
     SSMANLFSDF VSESSNGEYR VKEPRPVTQY RVEHDEPVAS GAPSAWWQVL VGITTAILGA
     IIFFLWRCFL RAKRVKFQAK DSFPWFTTSG DDDLPPPPGD SPSRPPGRSP DRVLPRTVVR
     DLSFNDDDDL HSVDLNEAGS RFGEVVSLIA RGNLRELAGA IPESLSNLTL LQTSASGSGF
     YTMVALYLAT LGDAITAFHE HNDASPATIQ SLRTLELQLE ARGLRFNEAG TPANLIQRGV
     KSSVGRALVR LTQSALLATG ENFRTRMAAT LERIAAERLN TLTAYDQRVI EMTTELLAAI
     KTALEVERSE LTPHLANAEA LLQVYNNLFS TDYASASLLA LRREMILRSA EGRVGEQPTS
     ASDAANEELV QRSMTKLDKE IELFQAQIDS QRRAVTITEA SNLRENILQP INTVANIAMA
     GAFLRGGARH RMPGIPDVAA PMSNPFRAFS GRGHSLTTTR GAGLFRRPRV