ID   POL2_BAYMJ              Reviewed;         890 AA.
AC   Q01207;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Genome polyprotein 2;
DE   Contains:
DE     RecName: Full=Helper component proteinase;
DE              Short=HC-pro;
DE              EC=3.4.22.45;
DE   Contains:
DE     RecName: Full=70 kDa protein;
GN   Name=RNA2;
OS   Barley yellow mosaic virus (strain Japanese II-1) (BaYMV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Stelpaviricetes;
OC   Patatavirales; Potyviridae; Bymovirus.
OX   NCBI_TaxID=31729;
OH   NCBI_TaxID=4513; Hordeum vulgare (Barley).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2016599; DOI=10.1099/0022-1317-72-4-995;
RA   Kashiwazaki S., Minobe Y., Hibino H.;
RT   "Nucleotide sequence of barley yellow mosaic virus RNA 2.";
RL   J. Gen. Virol. 72:995-999(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in
CC         the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the
CC         potyviral polyprotein.; EC=3.4.22.45;
CC   -!- PTM: The viral RNA2 of bymoviruses is expressed as a single polyprotein
CC       which undergoes post-translational proteolytic processing resulting in
CC       the production of at least two individual proteins. The HC-pro cleaves
CC       its C-terminus autocatalytically (Potential). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the bymoviruses polyprotein 2 family.
CC       {ECO:0000305}.
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DR   EMBL; D01092; BAA00876.1; -; Genomic_RNA.
DR   SMR; Q01207; -.
DR   MEROPS; C06.002; -.
DR   Proteomes; UP000007447; Genome.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.120.70; -; 1.
DR   Gene3D; 3.90.70.150; -; 1.
DR   InterPro; IPR001456; HC-pro.
DR   InterPro; IPR031159; HC_PRO_CPD_dom.
DR   InterPro; IPR042308; HC_PRO_CPD_sf.
DR   InterPro; IPR036417; TMV-like_coat_sf.
DR   Pfam; PF00851; Peptidase_C6; 1.
DR   SUPFAM; SSF47195; SSF47195; 1.
DR   PROSITE; PS51744; HC_PRO_CPD; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Thiol protease.
FT   CHAIN           1..255
FT                   /note="Helper component proteinase"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000040556"
FT   CHAIN           256..890
FT                   /note="70 kDa protein"
FT                   /id="PRO_0000040557"
FT   DOMAIN          135..255
FT                   /note="Peptidase C6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
FT   REGION          507..533
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        515..529
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        143
FT                   /note="For helper component proteinase activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
FT   ACT_SITE        215
FT                   /note="For helper component proteinase activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
FT   SITE            255..256
FT                   /note="Cleavage; by autolysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01080"
SQ   SEQUENCE   890 AA;  98464 MW;  5D0C70CE2AEFD5D0 CRC64;
     MSASSSRQLF DCGSLDWPNK SLFGDPTTRD VMHEHISSTW NAVIRRHMLA PNADAETILG
     RDGLPSAQFD AYGAMLPSFI QALNAPTTRL RITHRCPTAE SILCADASHA PWLYMANNVC
     AYEATHLKPV QTFIAFDFAH GYCYLSLFIP LSFRITFENA RSFSRFLEQL PDILGAYPTL
     AAIYKTMLFA IRLFPEVLQA PIPIIAKRPG VLQFHVSDAR GLPPSWFPMK CGSVRSFVAL
     ITNNLNSDLL DGIVGSNGDG EHYTNWNSGH DHWIVNRFIT VRDLHSSLKS ALDVDLDTEG
     GRNAVLDLLL DLGVTNLVRR EKRFPAHFQG AESVYLLLSC ERVGNELVAV QDALQEPLAN
     HSGLDLRALI INLGGLPSRH SDICYTRNIF ENDNHLVWNF EFYRIASITR NAQIDRDMLS
     SSMANLFSNF VSESSNGQYR VKEPRPIAQY RVEHDEPVAS GAPSAWWQVL IGITTAILGA
     IIFFLWRCFL RAKRVKFQAK DSFPWFTTSG DDDSPPPPGD SPSHPPGRSP DRVLPRTVVR
     DLSFNDDDDL HSVDLDEAGS RFGEVVSLIA RGNLRELAGA IPESLSNLTL LQTSASGSGF
     YTMVALYLAT LGDAITAFHE HNDASPTTTQ SLRTVELQLE ARGLRFNEAG TPANLIQRGV
     NSSVGRALVR LTQSALLATG EKFRTRMATT LERIAAERLN TLTAYDQRVI EMTTELLAAI
     KTALEVERSE LTPHLANAEA LLQVYNNLFS TDYASASLLA LRREMILRSA EGRVGEQPTS
     ASDAANEELV QRSMTKLDKE IELFQAQIDS QRRAVTITEA SNLRENILQP INTVANIAMA
     GAFLRGGARH RMPGMPDVAA PMPNPFRAFS GRGHSLTTTR SGGLFRRPRV