POL2_BBWVS
ID POL2_BBWVS Reviewed; 1018 AA.
AC Q76L39;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 23-FEB-2022, entry version 50.
DE RecName: Full=RNA2 polyprotein;
DE AltName: Full=119kDa protein;
DE AltName: Full=Genome polyprotein M;
DE Contains:
DE RecName: Full=VP53;
DE AltName: Full=53 kDa protein;
DE Contains:
DE RecName: Full=Movement protein;
DE Short=MP;
DE AltName: Full=37 kDa protein;
DE AltName: Full=VP37;
DE Contains:
DE RecName: Full=Large capsid protein;
DE Short=LCP;
DE AltName: Full=44 kDa protein;
DE Contains:
DE RecName: Full=Small capsid protein;
DE Short=SCP;
DE AltName: Full=22 kDa protein;
OS Broad bean wilt virus 1 (strain Spinach/United States/ATCC PV-132/1963)
OS (BBWV-1).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Picornavirales; Secoviridae; Comovirinae; Fabavirus.
OX NCBI_TaxID=649895;
OH NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH NCBI_TaxID=13163; Myzus.
OH NCBI_TaxID=4101; Petunia.
OH NCBI_TaxID=3562; Spinacia oleracea (Spinach).
OH NCBI_TaxID=3906; Vicia faba (Broad bean) (Faba vulgaris).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA Kobayashi Y.O., Kobayashi A., Nakano M., Hagiwara K., Honda Y., Omura T.;
RT "Analysis of genetic relations between Broad bean wilt virus 1 and Broad
RT bean wilt virus 2.";
RL J. Gen. Plant Pathol. 69:320-326(2003).
CC -!- FUNCTION: [VP53]: Acts as suppressor of post-transcriptional gene
CC silencing (PTGS), a mechanism of plant viral defense that limits the
CC accumulation of viral RNAs. Binds ssRNA.
CC {ECO:0000250|UniProtKB:Q9Q2Q3}.
CC -!- FUNCTION: [Movement protein]: Transports the viral genome to
CC neighboring plant cells directly through plasmosdesmata, without any
CC budding. The movement protein allows efficient cell to cell
CC propagation, by bypassing the host cell wall barrier. Acts by forming a
CC tubular structure at the host plasmodesmata, enlarging it enough to
CC allow free passage of virion capsids. Binds to GTP and to single-
CC stranded RNA and single-stranded DNA in a non-sequence-specific manner.
CC Also acts as suppressor of post-transcriptional gene silencing (PTGS),
CC a mechanism of plant viral defense that limits the accumulation of
CC viral RNAs. {ECO:0000250|UniProtKB:Q9Q2Q3}.
CC -!- FUNCTION: [Large capsid protein]: Together with the small capsid
CC protein, forms an icosahedral capsid (T=3) enclosing the viral positive
CC strand RNA genome, with a diameter of approximately 300 Angstroms. The
CC large capsid protein interacts with the viral RNA (By similarity). Also
CC acts as suppressor of post-transcriptional gene silencing (PTGS), a
CC mechanism of plant viral defense that limits the accumulation of viral
CC RNAs. Binds ssRNA (By similarity). {ECO:0000250|UniProtKB:P03599,
CC ECO:0000250|UniProtKB:Q9Q2Q3}.
CC -!- FUNCTION: [Small capsid protein]: Together with the large capsid
CC protein, forms an icosahedral capsid (T=3) enclosing the viral positive
CC strand RNA genome, with a diameter of approximately 300 Angstroms. The
CC capsid is formed from 60 copies each of the large and the small capsid
CC protein. The small capsid protein forms the turrets at the fivefold
CC axes of the viral particle. {ECO:0000250|UniProtKB:P03599}.
CC -!- SUBUNIT: [Small capsid protein]: Interacts with the large capsid
CC protein. {ECO:0000250|UniProtKB:P03599}.
CC -!- SUBUNIT: [Large capsid protein]: Interacts with the small capsid
CC protein. Homomultimer; assembles as pentons. Interacts with the
CC movement protein (via C-terminus). {ECO:0000250|UniProtKB:P03599}.
CC -!- SUBUNIT: [Movement protein]: Interacts (via C-terminus) with the large
CC capsid protein. {ECO:0000250|UniProtKB:P03599}.
CC -!- SUBCELLULAR LOCATION: [Movement protein]: Host endoplasmic reticulum
CC {ECO:0000250|UniProtKB:Q9Q2Q3}. Host cell junction, host plasmodesma
CC {ECO:0000250|UniProtKB:Q9Q2Q3}. Note=Assembles in tubules that are
CC embedded within modified plasmodesmata. {ECO:0000250|UniProtKB:P03599}.
CC -!- SUBCELLULAR LOCATION: [Large capsid protein]: Virion
CC {ECO:0000250|UniProtKB:P03599}.
CC -!- SUBCELLULAR LOCATION: [Small capsid protein]: Virion
CC {ECO:0000250|UniProtKB:P03599}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=RNA2 polyprotein; Synonyms=119kDa protein;
CC IsoId=Q76L39-1; Sequence=Displayed;
CC Name=RNA2 polyprotein 104kDa; Synonyms=104kDa protein;
CC IsoId=Q76L39-2; Sequence=VSP_059982;
CC -!- DOMAIN: [Movement protein]: The C-terminus is important for targeting
CC the movement protein to the plasmodesmata.
CC {ECO:0000250|UniProtKB:Q9Q2Q3}.
CC -!- DOMAIN: [VP53]: The N-terminus is involved in ssRNA-binding.
CC {ECO:0000250|UniProtKB:Q9Q2Q3}.
CC -!- PTM: [RNA2 polyprotein]: Specific enzymatic cleavages by picornain 3C-
CC like protease in vivo yield mature proteins.
CC {ECO:0000250|UniProtKB:P03599}.
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DR EMBL; AB084451; BAD00184.1; -; Genomic_RNA.
DR RefSeq; NP_945135.1; NC_005290.1.
DR SMR; Q76L39; -.
DR GeneID; 2658943; -.
DR KEGG; vg:2658943; -.
DR Proteomes; UP000000409; Genome.
DR GO; GO:0044165; C:host cell endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR GO; GO:0039617; C:T=3 icosahedral viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.20; -; 2.
DR InterPro; IPR003181; Como_LCP.
DR InterPro; IPR003182; RNA2_polyprotein.
DR InterPro; IPR029053; Viral_coat.
DR Pfam; PF02247; Como_LCP; 1.
DR Pfam; PF02248; Como_SCP; 1.
PE 3: Inferred from homology;
KW Alternative initiation; Capsid protein; Host cell junction;
KW Host endoplasmic reticulum; Reference proteome;
KW T=3 icosahedral capsid protein; Transport; Viral movement protein; Virion.
FT CHAIN 1..1018
FT /note="RNA2 polyprotein"
FT /id="PRO_0000402787"
FT CHAIN 1..419
FT /note="VP53"
FT /id="PRO_0000402788"
FT CHAIN 91..419
FT /note="Movement protein"
FT /id="PRO_0000445856"
FT CHAIN 420..821
FT /note="Large capsid protein"
FT /id="PRO_0000402789"
FT CHAIN 822..1018
FT /note="Small capsid protein"
FT /id="PRO_0000402790"
FT SITE 419..420
FT /note="Cleavage; by viral protease"
FT /evidence="ECO:0000250|UniProtKB:P03599"
FT SITE 821..822
FT /note="Cleavage; by viral protease"
FT /evidence="ECO:0000250|UniProtKB:P03599"
FT VAR_SEQ 1..90
FT /note="Missing (in isoform RNA2 polyprotein 104kDa)"
FT /id="VSP_059982"
SQ SEQUENCE 1018 AA; 114253 MW; 99D1B5179D30DEB2 CRC64;
MPLILLWFCF LCMIYVILKL EYTYVVKPFL KTAFLNTTSH YSDEYLREYS GIRWLHTFKE
YREHTIFFNF PSFYCSPGSG IKHTLSELIK MEAERVNLTK TETIPKDILL ERAKNYRVAQ
ESNKSLLPQV EDLYEVSKWK RAISSLQKGE PSFVRTSEVA IGTMSGAGKM RIKVPVVKSY
EEEVADMRLS QKVRAKADQI VVAAIEIVND GFASVNSDVT LAASLYDKRH KTIASSFKGA
YASRASGTPS HVVFYPTHRV APGDNPNDTL ELSAVSRDSD FDENFTLANF SVRTVYAKAK
GPEVIRETQH LLNCKLEDLV KAQQFASDEQ VVLALPRVYP KVNLDNYVMP GPDNVTKQEG
EYSAKGIHFR KPIFNGSEIV LNATSKLPSS KSRGISKSEK IDDLGCMSDE EGIDYKYGQA
LMEEDVLEAQ VDMFPLHNVA ETMRLLFSGV STIPMNVVEG TKISVAYLNE LATHPGVHVP
ILNMLGRIPG SILARVHCEV APTCGIGLAA TYVEGNESAA LGTDLGRLLG IQHVKWNPAI
EPVKEFRFKP FSCVDWWNMH YLGSSKFSPV LAFVCLSKWI NPPKGECKMS YALYFEPDII
LPRQIASLNS VPSFMLRKEL GTLSFKQGER RAYAFEVNFG KPQVEGKSVT LNFASAYCGL
SQYMESDIVI DLTLMSSPMM GGTFTLAYVA GSYLKSIKNM QFLDALPHIT FNFEKGGKST
RSLRFPSRLF PTYQSLDRWD LNASREDDVS GHFVLYQRDT VSSALEGDLV FRVSARVSGE
PMMHGVSVGY PTTLTRATTG KMSSRSLGEK VRKPIGLAKG QAHMNLADYK RVFYPMAEWI
YSSEKYEGRR EDRDILKLLL KMRLDGTKAT EDFRIVHSPL VRVLQNCAWL RGTIHFKIVV
RANSEMMSYQ RTSQVHVTAH ENSLSSNEFF SGMLTATSGE LEFSKEVVGP VEGFSSMGWN
VQGNKKFYKL CIALGNVHEY EAVKVMASFG DDVEFAGQQK AGHYALERGV SVFKEFKY
