ID   POL2_BRAV               Reviewed;        1626 AA.
AC   Q9YK98;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 2.
DT   23-FEB-2022, entry version 79.
DE   RecName: Full=RNA2 polyprotein;
DE   AltName: Full=P2;
DE   Contains:
DE     RecName: Full=Protein 2A;
DE   Contains:
DE     RecName: Full=Movement protein;
DE              Short=MP;
DE   Contains:
DE     RecName: Full=Coat protein;
DE              Short=CP;
OS   Blackcurrant reversion association virus (BRAV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Picornavirales; Secoviridae; Comovirinae; Nepovirus.
OX   NCBI_TaxID=65743;
OH   NCBI_TaxID=78511; Ribes nigrum (European black currant).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10564755; DOI=10.1016/s0168-1702(99)00105-7;
RA   Latvala-Kilby S., Lehto K.;
RT   "The complete nucleotide sequence of RNA2 of blackcurrant reversion
RT   nepovirus.";
RL   Virus Res. 65:87-92(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1045-1626, AND PROTEIN SEQUENCE OF 1094-1103.
RX   PubMed=9617765; DOI=10.1016/s0168-1702(97)00116-0;
RA   Latvala S., Susi P., Kalkkinen N., Lehto K.;
RT   "Characterization of the coat protein gene of mite-transmitted blackcurrant
RT   reversion associated nepovirus.";
RL   Virus Res. 53:1-11(1998).
CC   -!- FUNCTION: [Protein 2A]: Implicated in RNA2 replication. Could also be
CC       required for nematode transmission of the virus (By similarity).
CC       {ECO:0000250}.
CC   -!- FUNCTION: [Movement protein]: Transports viral genome to neighboring
CC       plant cells directly through plasmosdesmata, without any budding. The
CC       movement protein allows efficient cell to cell propagation, by
CC       bypassing the host cell wall barrier. Acts by forming a tubular
CC       structure at the host plasmodesmata, enlarging it enough to allow free
CC       passage of virion capsids (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [Movement protein]: Host cell junction, host
CC       plasmodesma {ECO:0000250}. Note=Assembles in tubules that are embedded
CC       within modified plasmodesmata. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [Coat protein]: Virion {ECO:0000305}.
CC   -!- PTM: Specific enzymatic cleavages in vivo by the P1 encoded 3C-like
CC       protease yield mature proteins. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Virions are comprised of 60 copies of the coat protein.
CC   -!- SIMILARITY: Belongs to the nepoviruses RNA2 polyprotein family.
CC       {ECO:0000305}.
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DR   EMBL; AF020051; AAD09329.2; -; mRNA.
DR   RefSeq; NP_612586.1; NC_003502.1.
DR   SMR; Q9YK98; -.
DR   PRIDE; Q9YK98; -.
DR   GeneID; 993415; -.
DR   KEGG; vg:993415; -.
DR   Proteomes; UP000007614; Genome.
DR   GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.20; -; 2.
DR   InterPro; IPR005054; Nepo_coat.
DR   InterPro; IPR005305; Nepo_coat_C.
DR   InterPro; IPR005306; Nepo_coat_N.
DR   InterPro; IPR029053; Viral_coat.
DR   Pfam; PF03391; Nepo_coat; 1.
DR   Pfam; PF03688; Nepo_coat_C; 1.
DR   Pfam; PF03689; Nepo_coat_N; 1.
PE   1: Evidence at protein level;
KW   Capsid protein; Direct protein sequencing; Host cell junction; Transport;
KW   Viral movement protein; Virion.
FT   CHAIN           1..?
FT                   /note="Protein 2A"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000037124"
FT   CHAIN           ?..1093
FT                   /note="Movement protein"
FT                   /id="PRO_0000037125"
FT   CHAIN           1094..1626
FT                   /note="Coat protein"
FT                   /id="PRO_0000037126"
FT   REGION          84..107
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1042..1066
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        90..104
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1626 AA;  178806 MW;  350C5BB30938263B CRC64;
     MSESGNTTSM PGCGRMCALR STWSKRAFLV ACKDGALTSD GRCPQYGCGA LVSITKGVQQ
     PKKTASAKVV KCLCWVQPAR WCEKHSKGPA SPNGSVTTKR SNSARAAPAP LPYKKQTCDV
     VVTVGPLELV YPALVSEELP TPVAATPTKV EEVPIPELPL WLAPAWMVEQ PYAATPEVLC
     LTQREEFALL KKRLTRKGKL LQRRATHARF EARAALARVR AATQRKVEEV TALVIKGRRI
     LAAHQLLREL EEVAPLSQAQ EQLVASSCAA AAARQEECAS FLRRAKAWRK SISATPPVAF
     ATAVASKVVS ATMPWAHLGL SLGGLLAVPT LDGTLGAKQW NAKTIATWVL KPVVSCVQSV
     HAKVRDWLHS QPEVGVTNTK VPLVLPEVCL GVLSPPSLSE EIVDNPQETS QSGIWHPEMG
     VRNIYVFHDD SWETSPEEDE NYTYTFSRQC GIPYLLVEGR GAEERKNTIL GWDFSLHNDG
     EFEFLPSPEE GYTKELVTPV ALEEEDKYST ASSCGFFSLD DVSSAITIQC PGLLSADADV
     HFFDGPGYRC SSRPRDFRPP VVRGCDYESR VKASIQRKIE NPLQERFITV LREKRKKNKK
     KEFHSFSACF AFKRKQIQWP PTPNEMVNEW EEYCIAQAWL PFEVVVTDEI EDVTPLYPGG
     RDYNCNSQLL FPLAPLSTVY CDDSCFHPND GWTTDGNGKH FRLSPQFVLP DVPIPIVHRV
     TRQLPQFLYD LGIGDLTCNS GYQAENLQEE IQERMEDRSE EKPVPSLDTL ISKLSKRSTK
     VKGAGENRYA DRHSLTEKAI FHQPGALSRM RSGKEKTIVA ANHNSDQISV RMAECGKPVF
     TPLPRMSDEM LRKFLEKGLG STSTVALDIG IQSHIPQGMP TVAFVNVMDT RIEDPLYSSL
     CGSYIDLGRD RAKTLCLPLV NFPMSKLAED VDDVLNGLML CTHFQDSTKF GVGKPAFQYG
     TLEFQEFKPS AYSDFSRVRD NWDAIAKQQN TPNDRILAGF SVLGAVSQAY NQALPVFKSV
     ELVAPPKRKP VVATFQNPTT LGRSNTTRSF RMPTMDLPRS TGRDAPIPIV HRRNNNDVHG
     FDEATPARFS TCDSGLVADT TLAFAKMYQC KKDAKAGHVL ATIDIQECVF EDNRRVALDW
     LAHGLASFKY DLQLTVDSNP FVGVTLGITV DAFDRLLPQI SDEVIAVPLA FQLPTYLFPI
     SKKGTFTQTI DFAAIAGYNF FPHVAAFGRP KIIVYIVSDN DLPASDTWMC LVELHMTRLE
     SSTLACSPTL VLPQAFGGDL PLDLWRGPYT FPLGGGTKRL STSLDIGTST TTVSGWRTVS
     FPAAYALFLQ GHGGSLVGEV VHTGSAAVSC ALHLCISFGG APPTLEEALV FPGFRLPSGE
     GKFHIKVQTP YGRLSTLTPD CALYVYLAGG PIAVAPMSVP YQFCIHLERL VDDGAPPRTI
     GLIREFNWAT INNFKSDDIT FAIPARLSDL VLTCGDVTMS TNPLALLIGS CGFFRGNLTV
     VLEWATFLKA GDKEGTVQLT TCRGMINNVK GVRNAIQKKV VNLSLVGSVS RYLNVGDFTG
     FAQSGGQVGY DEIFLEFSTN KAKQIRYLNI NVELDENFEL YGRTIIPLKN TAPAFASTSA
     SAPNES