ID   POL2_BRSV               Reviewed;        1357 AA.
AC   P14547;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   23-FEB-2022, entry version 93.
DE   RecName: Full=RNA2 polyprotein;
DE   AltName: Full=P2;
DE   Contains:
DE     RecName: Full=Protein 2A;
DE              Short=P2A;
DE   Contains:
DE     RecName: Full=Movement protein;
DE     AltName: Full=2B-MP;
DE   Contains:
DE     RecName: Full=Coat protein;
DE     AltName: Full=2C-CP;
OS   Beet ringspot virus (BRSV) (Tomato black ring virus (strain S)).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Picornavirales; Secoviridae; Comovirinae; Nepovirus.
OX   NCBI_TaxID=191547;
OH   NCBI_TaxID=4681; Allium porrum (Leek) (Allium ampeloprasum var. porrum).
OH   NCBI_TaxID=4045; Apium graveolens (Celery).
OH   NCBI_TaxID=161934; Beta vulgaris (Sugar beet).
OH   NCBI_TaxID=38871; Fraxinus (ash trees).
OH   NCBI_TaxID=4236; Lactuca sativa (Garden lettuce).
OH   NCBI_TaxID=39639; Narcissus pseudonarcissus (Daffodil).
OH   NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH   NCBI_TaxID=35938; Robinia pseudoacacia (Black locust).
OH   NCBI_TaxID=23216; Rubus (bramble).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
OH   NCBI_TaxID=13305; Tulipa.
OH   NCBI_TaxID=3603; Vitis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Meyer M., Hemmer O., Mayo M.A., Fritsch C.;
RT   "The nucleotide sequence of tomato black ring virus RNA-2.";
RL   J. Gen. Virol. 67:1257-1271(1986).
CC   -!- FUNCTION: [Protein 2A]: Implicated in RNA2 replication. Could also be
CC       required for nematode transmission of the virus (By similarity).
CC       {ECO:0000250}.
CC   -!- FUNCTION: [Movement protein]: Transports viral genome to neighboring
CC       plant cells directly through plasmosdesmata, without any budding. The
CC       movement protein allows efficient cell to cell propagation, by
CC       bypassing the host cell wall barrier. Acts by forming a tubular
CC       structure at the host plasmodesmata, enlarging it enough to allow free
CC       passage of virion capsids (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cell junction, host plasmodesma.
CC       Note=Assembles in tubules that are embedded within modified
CC       plasmodesmata (By similarity). Movement proteins are targeted
CC       preferentially to calreticulin-labeled foci within the youngest cross
CC       walls, where they assemble into tubules. During cell division, they
CC       colocalize in the cell plate with KNOLLE, a cytokinesis-specific
CC       syntaxin (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [Protein 2A]: Host cytoplasm {ECO:0000250}. Host
CC       nucleus {ECO:0000250}. Note=Cytoplasmic early in infection. Later in
CC       infection, it becomes progressively concentrated around the nucleus,
CC       where it forms large aggregates (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [Coat protein]: Virion {ECO:0000305}.
CC   -!- PTM: Specific enzymatic cleavages in vivo by the P1 encoded 3C-like
CC       protease yield mature proteins. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Virions are comprised of 60 copies of the coat protein.
CC   -!- SIMILARITY: Belongs to the nepoviruses RNA2 polyprotein family.
CC       {ECO:0000305}.
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DR   EMBL; X04062; CAA27694.1; -; Genomic_RNA.
DR   RefSeq; NP_620113.1; NC_003694.1.
DR   SMR; P14547; -.
DR   GeneID; 988050; -.
DR   KEGG; vg:988050; -.
DR   Proteomes; UP000007615; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.20; -; 2.
DR   InterPro; IPR005054; Nepo_coat.
DR   InterPro; IPR005305; Nepo_coat_C.
DR   InterPro; IPR005306; Nepo_coat_N.
DR   InterPro; IPR029053; Viral_coat.
DR   Pfam; PF03391; Nepo_coat; 1.
DR   Pfam; PF03688; Nepo_coat_C; 1.
DR   Pfam; PF03689; Nepo_coat_N; 1.
PE   3: Inferred from homology;
KW   Capsid protein; Host cell junction; Host cytoplasm; Host nucleus;
KW   Transport; Viral movement protein; Virion.
FT   CHAIN           1..?440
FT                   /note="Protein 2A"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000037115"
FT   CHAIN           ?441..837
FT                   /note="Movement protein"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000037116"
FT   CHAIN           838..1357
FT                   /note="Coat protein"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000037117"
SQ   SEQUENCE   1357 AA;  150010 MW;  916E821C4B8826E4 CRC64;
     MGFRELFASS LGDAARAKAS LVRGMSGWLN TTLATVQAAG PEIRACAYSA LWAEVDSVKE
     LVPLSATMLL NQLRADLKCA QVRAQKCTPA STSRFCSCGG IPLPGENKWE DELVPIPDCP
     VGRNFCRDGV FCKRHHGPGE TLERVQVLVE APKCPHCQGT GIIPRSEPMA YIRSEYERQT
     KTFARPSNPL HEWILEEGRE SNHARRCSNW YHMSMSQIDK RDNDPCDAST WMAAAQILFD
     NADAQVYYPG SNYRKLVGGS KGYDDWCQLP PSKEMCNRLF DWWHRQNTPD YYVSEDTLAD
     FVKPRMGSCH LPIEHDVHLL PREWHGRVSA GNTSHFMACL DGLSSSMEEF LDVFYDCAAQ
     FNGDIEIFLD TNEKPSRVVG NLGGVRVLLT TPAVCSPAKL LPEIEESDFD QLEDESISCD
     SMIPPLFRDN GLSALYANLV LKQATVQSIL MAHPDQDEIE DQVDHLENKQ GGEIVTTPAF
     IKMLKEKRKE VRGKEFAEGS EGRLVRASDL TLSREDVFLS GGLFEKFRKS GIVQTFKGKD
     PKLTKVCVDL TNSQEIIKYP SKEMCSDSSG VHTGQVFTVL NRPLYNELNK LAESGWKEAK
     SVCLNLHIRS YVPVHTPLYA FCVIMWGHSS DAETASLCGA GVYLGDQEAA VLELPLVCSY
     LGNSLEDFDA YKRSLVLSTV FFGKSGLFAG QNVFGITAVE FTEYMPTSYG GITHERDSWQ
     AMLRNHQGKD KGRFIAGFNV VDALERDKEE PIKMPNLDLE PVPRTQPIVR TFTGEGKQPL
     LNKSRSMRIQ SFVSFRGSNI PVGRRIDNTA EAINYELGRA STSSVSSRLD ESKCNLKAGG
     SYAFGETIEL PATVTPGTVL AVFNIFDKIQ ETNTKVCSKW LEQGYVSQNL TAISHLAPNA
     FSGIAIWYIF DAYGKIPGDV TTTFELEMAR SFDPHVQVLR DVSTSTWVID FHKICGQTLN
     FSGQGYCVPK IWVIAASTFQ LARSTATKFR LEFYTRGEKL VRGLAEQPLS YPIEARHLTD
     LNLMLAPKQI AVGTYAMITF PVSLAAKLQS TSGRTAYSYA AGLLSHFLGV GGTIHFVVRT
     TSSAFVTSKL RIALWGTVPE TDQLAQMPHV DVEVNVDASL QIQSPFFSTA NFGNSGSAFY
     VSTLCAPMAP ETVETGSEYY IQIKGIEANP GLCREINYKQ RFAWCLLECL DNSKASPIKV
     KIPSRIGNLS SKHVKVTNFV NALAILCATT GMHHGNCTIH FSWLWHPAEL GKQLGRLKFV
     QGMGINNEHI GDTMCYNSLS NTHSVPFQFG SFAGPITSGG KADEAENWIE IQSPDFSWVA
     SLHVSIEVHE GFKFYGRSAG PLTIPATVAD VSAVSGS