ID   POL2_CNSV               Reviewed;        1240 AA.
AC   Q8QVU9;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   23-FEB-2022, entry version 74.
DE   RecName: Full=RNA2 polyprotein;
DE   AltName: Full=P2;
DE   Contains:
DE     RecName: Full=Protein 2A;
DE              Short=P2A;
DE   Contains:
DE     RecName: Full=Movement protein;
DE     AltName: Full=2B-MP;
DE   Contains:
DE     RecName: Full=Coat protein;
DE     AltName: Full=2C-CP;
OS   Cycas necrotic stunt virus (CNSV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Picornavirales; Secoviridae; Comovirinae; Nepovirus.
OX   NCBI_TaxID=173976;
OH   NCBI_TaxID=16901; Aucuba japonica (Japanese laurel) (Spotted laurel).
OH   NCBI_TaxID=3396; Cycas revoluta (Sago palm).
OH   NCBI_TaxID=49747; Gladiolus.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA], AND PROTEIN SEQUENCE OF 684-695.
RX   PubMed=12417954; DOI=10.1007/s00705-002-0876-5;
RA   Han S.S., Karasev A.V., Ieki H., Iwanami T.;
RT   "Nucleotide sequence and taxonomy of Cycas necrotic stunt virus.";
RL   Arch. Virol. 147:2207-2214(2002).
CC   -!- FUNCTION: [Protein 2A]: Implicated in RNA2 replication. Could also be
CC       required for nematode transmission of the virus (By similarity).
CC       {ECO:0000250}.
CC   -!- FUNCTION: [Movement protein]: Transports viral genome to neighboring
CC       plant cells directly through plasmosdesmata, without any budding. The
CC       movement protein allows efficient cell to cell propagation, by
CC       bypassing the host cell wall barrier. Acts by forming a tubular
CC       structure at the host plasmodesmata, enlarging it enough to allow free
CC       passage of virion capsids (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [Movement protein]: Host cell junction, host
CC       plasmodesma {ECO:0000250}. Note=Assembles in tubules that are embedded
CC       within modified plasmodesmata. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [Coat protein]: Virion {ECO:0000305}.
CC   -!- PTM: Specific enzymatic cleavages in vivo by the P1 encoded 3C-like
CC       protease yield mature proteins. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Virions are comprised of 60 copies of the coat protein.
CC   -!- SIMILARITY: Belongs to the nepoviruses RNA2 polyprotein family.
CC       {ECO:0000305}.
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DR   EMBL; AB073148; BAB89370.2; -; Genomic_RNA.
DR   RefSeq; NP_620620.2; NC_003792.2.
DR   SMR; Q8QVU9; -.
DR   GeneID; 988024; -.
DR   KEGG; vg:988024; -.
DR   Proteomes; UP000008564; Genome.
DR   GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.20; -; 2.
DR   InterPro; IPR005054; Nepo_coat.
DR   InterPro; IPR005305; Nepo_coat_C.
DR   InterPro; IPR005306; Nepo_coat_N.
DR   InterPro; IPR029053; Viral_coat.
DR   Pfam; PF03391; Nepo_coat; 1.
DR   Pfam; PF03688; Nepo_coat_C; 1.
DR   Pfam; PF03689; Nepo_coat_N; 1.
PE   1: Evidence at protein level;
KW   Capsid protein; Direct protein sequencing; Host cell junction; Transport;
KW   Viral movement protein; Virion.
FT   CHAIN           1..?314
FT                   /note="Protein 2A"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000037118"
FT   CHAIN           ?315..683
FT                   /note="Movement protein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000037119"
FT   CHAIN           684..1240
FT                   /note="Coat protein"
FT                   /id="PRO_0000037120"
FT   REGION          1193..1240
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1193..1214
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1240 AA;  138745 MW;  334E35EE5B67A0D8 CRC64;
     MFAPIGAPGM GERASQNFRS ASGFRDLLVH AVKVLVLSAY QRRPCTSMLR PREQKRIKMR
     LKWMCLMKFC FMQNNYWDTQ RRFTGGMHNV PELATFDAWL QHWRVVHWRL NIISEILIDF
     QALVRMMKNL AGQCHFDFYP VCTHCVQLVN NWYIAEFKPH LEEETEWWKD LVKPRMGNPV
     HEIEPNRKSQ YVESRRIPPP LNGDEFANFL HVCQCAKLAF DVERAATEEN FEDALDTLEE
     DFYDIDSTIP KRDLLAADAR VVKRAFTLRR KRRPNRTSVY SMKGQPPVTF SASDLVSCLG
     QVLSTLPTVK MDREAIEDQQ DHLEDKQGGE ILTTPQFIEV LRKKKREVRE KEFDDSTQGK
     LLPAEDFTLS KHDVFLANSV LDGLRKSKLI QRFAGKCATS TKITVDLTNK EEVVRYGPKE
     LASEGFRQTF NVLNRPEYNA LNKLAEAGWK EAKSVVLNLH IRSYLPQQMN AYAFCVIMWG
     HSSDAQEAAL SGSYVYLGDG EATMLQLPLL CEYVGHNLQD FEAYKRSLVL STVFPEFSGI
     ADGKAMFGIT SIEFTEYLPT SHAGITHERD SWDAMLRNHT EEKRRFLAGF NVVDTIEKGN
     RKGFSFPDFD LKAVPRHQAV VRTFEDQDVA PILSKAKSMR VKTFGSFRAG NIPVNFLGTP
     SNGQVASKHS VSENAGYSVG DMKSAENFVF TQLITVPAAS TKGNVLAGVD ILANARTTMS
     GFYMRWLQKG YIDTNLKLIC HLPRAPFAGM SFFVLIDGTG YLAKDAPTSL NEEEILSYPL
     HLVTTSDVSS YEFVLDWHRY IGQVPFAEEN AFLRPTLFLV ACVSSTLALS AKVEFYLEAQ
     SVGEELPRTL APSPVLSYPF QNSFLEDLDL FLPPKRLTLG ERETTIIPLS FAKSKKSGDA
     VLYSHAAARL AHFQGIGGVL HGVVYLVGSQ LVASQSRISM WSKEQHIQHQ AVNVHVDTDT
     GVAFDLPIKD AFYASSVYGD SGAVIQVTCL CSPMSPNAIK APFDMIFKIR GFTPDAPMCR
     TINFTQRFGW FAVEPTTSTG AIKLKIWPVS NHLESEDMKV TGYTNAFLQM CQTSTMHFGS
     VIIHFSWTLF GGTTNAATAG GVVTIAEGFG PEEENFRGHC RNLSIYEGRA TVPLELGTFA
     GPTPLKKLDF KYRNWIRFTT PKGRNISSIF CAIEVLPGFS FYGRTGSPRL SVVGTTVPPT
     ADASTSNSQG GDEDIGDQYS AALGRGRGRG SRPGPSPIRG