POL2_GCMV
ID POL2_GCMV Reviewed; 1324 AA.
AC P13026;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 23-FEB-2022, entry version 94.
DE RecName: Full=RNA2 polyprotein;
DE AltName: Full=P2;
DE Contains:
DE RecName: Full=Protein 2A;
DE Short=P2A;
DE Contains:
DE RecName: Full=Movement protein;
DE AltName: Full=2B-MP;
DE Contains:
DE RecName: Full=Coat protein;
DE AltName: Full=2C-CP;
OS Grapevine chrome mosaic virus (GCMV) (Hungarian grapevine chrome mosaic
OS virus).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Picornavirales; Secoviridae; Comovirinae; Nepovirus.
OX NCBI_TaxID=12273;
OH NCBI_TaxID=4045; Apium graveolens (Celery).
OH NCBI_TaxID=29760; Vitis vinifera (Grape).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA], AND PROTEIN SEQUENCE OF 814-822.
RX PubMed=2798129; DOI=10.1093/nar/17.19.7809;
RA Brault V., Hibrand L., Candresse T., le Gall O., Dunez J.;
RT "Nucleotide sequence and genetic organization of Hungarian grapevine chrome
RT mosaic nepovirus RNA2.";
RL Nucleic Acids Res. 17:7809-7819(1989).
CC -!- FUNCTION: [Protein 2A]: Implicated in RNA2 replication. Could also be
CC required for nematode transmission of the virus (By similarity).
CC {ECO:0000250}.
CC -!- FUNCTION: [Movement protein]: Transports viral genome to neighboring
CC plant cells directly through plasmosdesmata, without any budding. The
CC movement protein allows efficient cell to cell propagation, by
CC bypassing the host cell wall barrier. Acts by forming a tubular
CC structure at the host plasmodesmata, enlarging it enough to allow free
CC passage of virion capsids (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host cell junction, host plasmodesma.
CC Note=Assembles in tubules that are embedded within modified
CC plasmodesmata (By similarity). Movement proteins are targeted
CC preferentially to calreticulin-labeled foci within the youngest cross
CC walls, where they assemble into tubules. During cell division, they
CC colocalize in the cell plate with KNOLLE, a cytokinesis-specific
CC syntaxin (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Protein 2A]: Host cytoplasm {ECO:0000250}. Host
CC nucleus {ECO:0000250}. Note=Cytoplasmic early in infection. Later in
CC infection, it becomes progressively concentrated around the nucleus,
CC where it forms large aggregates (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Coat protein]: Virion {ECO:0000305}.
CC -!- PTM: Specific enzymatic cleavages in vivo by the P1 encoded 3C-like
CC protease yield mature proteins. {ECO:0000250}.
CC -!- PTM: The N-terminus of the coat protein is blocked.
CC -!- MISCELLANEOUS: Virions are comprised of 60 copies of the coat protein.
CC -!- SIMILARITY: Belongs to the nepoviruses RNA2 polyprotein family.
CC {ECO:0000305}.
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DR EMBL; X15163; CAA33255.1; -; Genomic_RNA.
DR PIR; S06187; S06187.
DR RefSeq; NP_619704.1; NC_003621.1.
DR SMR; P13026; -.
DR GeneID; 956640; -.
DR KEGG; vg:956640; -.
DR Proteomes; UP000008624; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.20; -; 2.
DR InterPro; IPR005054; Nepo_coat.
DR InterPro; IPR005305; Nepo_coat_C.
DR InterPro; IPR005306; Nepo_coat_N.
DR InterPro; IPR029053; Viral_coat.
DR Pfam; PF03391; Nepo_coat; 1.
DR Pfam; PF03688; Nepo_coat_C; 1.
DR Pfam; PF03689; Nepo_coat_N; 1.
PE 1: Evidence at protein level;
KW Capsid protein; Direct protein sequencing; Host cell junction;
KW Host cytoplasm; Host nucleus; Transport; Viral movement protein; Virion.
FT CHAIN 1..?
FT /note="Protein 2A"
FT /evidence="ECO:0000250"
FT /id="PRO_0000037121"
FT CHAIN ?..810
FT /note="Movement protein"
FT /evidence="ECO:0000250"
FT /id="PRO_0000037122"
FT CHAIN 811..1324
FT /note="Coat protein"
FT /evidence="ECO:0000250"
FT /id="PRO_0000037123"
FT VARIANT 400
FT /note="N -> D"
FT VARIANT 1006
FT /note="I -> T"
SQ SEQUENCE 1324 AA; 147805 MW; 302C286B2EA1756A CRC64;
MGFSEFFASA LGTVARAKAT LQGGFARFLS ETVVTLQAAS PEMRKFAYSK LWEEVDSVKE
LKPLTAQELV ATLRKELWCA QVRAQKCTLA STSRFCTCGG IPGEATPTVI KETVHVDECP
NGRNLCRHGT RCLRHGGPGS FQQEREVQVD APKCPHCAGT GIVPASASWR EIRRCWREQR
KVHSLPSLPL HPDVLFEGTN AWQTRLRWLK TWRHVLGDVK PCTPEKWMQA AQIMRTCAVP
SFENPIPGQF GYERLYNGEG KEEYWLQIPA TDKYTDLIIN WWHAKNTPGW EEPSSSLMDF
KRNRMGPCLH IVEKRVRNSY VAPPWKPWGE DIDILSVMDS LSSQLEDFLD VFYDCAAQFD
GELEFSLSND RLSSVTGELG GVPISIGAPS KISNTPPKVN FAELYGNLVR HNHRKISALR
PILMAHPDQD EIEDQLDHLE NKQGGEIVST PSFIKMLKEK RKEVRGKEFE EGSEGRLVRS
KDLELSKKDI FLAHTLMDKF HGMSIVKKFG KSDPKLTKVC VDLTNQEEVI KYPVKELQTT
SEGVLSAQTF TVLNRPQFKE LNRLAEVGWK EAKSVCLNLH IRSYLPVHLP VYAFCVIMWG
HSSNAEQASL SGAYVYLGDQ EASVLQLPLL CGYIGNALED MEAYKRSLVL STCFFGTSGL
SPGQNMFGIT AVEFTEYLPT SYGGITHERD SWNQMLRNHQ GVDKQRFISG FNVVDFVEAG
KEKQLHFPDF DLQPVPKHQP IVRTFGKEKQ PLLNKSRSMR VKTFTSFRAG NIPIGRQIDN
TAEAINFELG RASTSNAINP RLDTSETNLR AGGEFAFIHT IDLPTAVTEG QVLAKIDIFK
KIQDAKSMVC VQWMQAGYVN KNLTFISHLA PSQFCGVAIW YIFDAYGKIP SDVTTSLELE
IARSLCPHVH VLRDSKTSVW TIDFHKICGQ SLNFSGRGFS KPTLWVIAAS TAQLPWSAQV
TYRLEALAQG DEIAHGLATR SIVTYPISLE HLKDIEIMLP PRQMAIGNAG SINFPLSFAV
QQKSSSGRIA YSYAAGLLSH FLGIGGTIHF KIQCTSSAFV TARLRVALWG DTITLEQLSQ
MPHVDCDVDV VSSLKIQSPF YATANFGDSG ARFWVTPMSS PMAPETMESK LEYYIQILGI
DADPPMCRQI NYDQRFAWFT LLRPPDPKLS KILKLTLPSR VCNIAYKEAT VTNYVNAFAI
MCATTGMHAG KCILHFSWTL NKGTSFKDLQ GHISFYSGMG DSTIGEHHGE FHLGGPLSSS
LAVPFEFGSF AGPVTSGGTP FTSENWLRVE TAHWDWLTSL TVDIQVLPGF RFYGRSAGPL
TIPS
