POL2_RRVS
ID POL2_RRVS Reviewed; 1107 AA.
AC P36324;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 23-FEB-2022, entry version 83.
DE RecName: Full=RNA2 polyprotein;
DE AltName: Full=P2;
DE Contains:
DE RecName: Full=Protein 2A;
DE Short=P2A;
DE Contains:
DE RecName: Full=Movement protein;
DE AltName: Full=2B-MP;
DE Contains:
DE RecName: Full=Coat protein;
DE AltName: Full=2C-CP;
OS Raspberry ringspot virus (strain S) (RpRSV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Picornavirales; Secoviridae; Comovirinae; Nepovirus.
OX NCBI_TaxID=36399;
OH NCBI_TaxID=57918; Fragaria vesca (Woodland strawberry) (Potentilla vesca).
OH NCBI_TaxID=39639; Narcissus pseudonarcissus (Daffodil).
OH NCBI_TaxID=32247; Rubus idaeus (Raspberry).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA], PROTEIN SEQUENCE OF 594-608, AND
RP PROTEOLYTIC PROCESSING OF POLYPROTEIN.
RX PubMed=1402811; DOI=10.1099/0022-1317-73-9-2189;
RA Blok V.C., Wardell J., Jolly C.A., Manoukian A., Robinson D.J.,
RA Edwards M.L., Mayo M.A.;
RT "The nucleotide sequence of RNA-2 of raspberry ringspot nepovirus.";
RL J. Gen. Virol. 73:2189-2194(1992).
CC -!- FUNCTION: [Protein 2A]: Implicated in RNA2 replication. Could also be
CC required for nematode transmission of the virus (By similarity).
CC {ECO:0000250}.
CC -!- FUNCTION: [Movement protein]: Transports viral genome to neighboring
CC plant cells directly through plasmosdesmata, without any budding. The
CC movement protein allows efficient cell to cell propagation, by
CC bypassing the host cell wall barrier. Acts by forming a tubular
CC structure at the host plasmodesmata, enlarging it enough to allow free
CC passage of virion capsids (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host cell junction, host plasmodesma.
CC Note=Assembles in tubules that are embedded within modified
CC plasmodesmata (By similarity). Movement proteins are targeted
CC preferentially to calreticulin-labeled foci within the youngest cross
CC walls, where they assemble into tubules. During cell division, they
CC colocalize in the cell plate with KNOLLE, a cytokinesis-specific
CC syntaxin (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Protein 2A]: Host cytoplasm {ECO:0000250}. Host
CC nucleus {ECO:0000250}. Note=Cytoplasmic early in infection. Later in
CC infection, it becomes progressively concentrated around the nucleus,
CC where it forms large aggregates (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Coat protein]: Virion {ECO:0000305}.
CC -!- PTM: Specific enzymatic cleavages in vivo by the P1 encoded 3C-like
CC protease yield mature proteins. {ECO:0000250}.
CC -!- MISCELLANEOUS: Virions are comprised of 60 copies of the coat protein.
CC -!- SIMILARITY: Belongs to the nepoviruses RNA2 polyprotein family.
CC {ECO:0000305}.
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DR EMBL; S46011; AAB23551.1; -; Genomic_RNA.
DR PIR; JQ1658; JQ1658.
DR SMR; P36324; -.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.20; -; 2.
DR InterPro; IPR005054; Nepo_coat.
DR InterPro; IPR005305; Nepo_coat_C.
DR InterPro; IPR005306; Nepo_coat_N.
DR InterPro; IPR029053; Viral_coat.
DR Pfam; PF03391; Nepo_coat; 1.
DR Pfam; PF03688; Nepo_coat_C; 1.
DR Pfam; PF03689; Nepo_coat_N; 1.
PE 1: Evidence at protein level;
KW Capsid protein; Direct protein sequencing; Host cell junction;
KW Host cytoplasm; Host nucleus; Transport; Viral movement protein; Virion.
FT CHAIN 1..?
FT /note="Protein 2A"
FT /evidence="ECO:0000250"
FT /id="PRO_0000037109"
FT CHAIN ?..593
FT /note="Movement protein"
FT /id="PRO_0000037110"
FT CHAIN 594..1107
FT /note="Coat protein"
FT /id="PRO_0000037111"
FT REGION 553..584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 1032
FT /note="A -> T"
SQ SEQUENCE 1107 AA; 123509 MW; FF65E0FA20026E6B CRC64;
MSQFWGEFPE KVIQTFQHLQ VALIGDIKKC ALSSPLFPEL SKLDAHSQHH LLASFELPRF
GGVTPGVMEQ LRDAESELAE AKQRLLRERL HAVANRQNIP YLGDCMYYDA PGISQEELLQ
AAFLEAPTPA WEHERIRPLW PKDEWFRDAR QGPYLEDYGN IPLGDLDTLC LAFDALVEEH
WMPIYLLIST FSMFQQYGTQ PLLLECAQSA GSLIPACMMT DHHLEPTGDR QADKEARQDY
ADSQDSIQSM GDFWKEFYTK DSGKKIPDSH KSRLANDPNK VGFTKSALFH KQPLSHSLAQ
TWANFRGTQD KADLVKVTMD MNIEKYTVRL PDAVRTTAGP LYIEWINLPR MSENSARKLA
EVGWNNADIC GVDLAVKSHI AVGTPVRVII SLVDGACSDM PTATMCAFEV NLASQNNRSL
NLPLLSLPFS RLLADLHDFQ NRVKIACQFR DPEGFNVGTP MLSFSSLEFS ELKQTAFERN
SLLRDSWSEI EKRACHGGGR CVASQGIVQT WEKEVNPPLK EYAPLVLPPV PQPKRNFIDQ
QTGEVVQSFM QKSRSMRFKS PSDLWSRPSV DGGSTSTQPP SKGSLRCENV PGCAYEVDPL
HLLYYESVDV PKDTLAGTLL ARIDVRAKAA IFDSAVWRQW VRDGCLKPKI KMRITAATSC
FSGIVLGACF DAYRRIPAAT KTGITASLVT GLPNTVWATR DTSEVEWDID LAAVCGHTFF
ALEDTFGYMD FLIYVLRGNE ITAVADWSIY VSFHVDWTQE SMLATLIPTF VWPPKPTDIS
LLKEVWGPYR FTLDGTEAKE SFAIMPGTAI LHGQQIVRTF PRVVAAHFRS WTGKVRMSIQ
EVSSIFLTGT YMVGVSWNAT ADLADIVTRK HWIVKSNEIF EVDLYCPYGE NPTFTGQANG
KPFIIVHKLG GIVGPKDSVG TFGFMIHIHG LTGVYKNPTL HSGDRSVGSA WFRINNIADD
NLVFNIPGRI EDIIAAAGKY DVTNYVNPTS LLFSVTGLHG GIIRLHITWC PNTTLGESKG
TLKYMQYLYH TATENFFGDQ ATRGIIDQDG FTIDIACGDF FGATRVGLPG EVERLGIYSS
NAKSIAEIRV SFEVLSMNFY GSTIKVT
