ID   POL2_TORVR              Reviewed;        1882 AA.
AC   P25247;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   23-FEB-2022, entry version 94.
DE   RecName: Full=RNA2 polyprotein;
DE   AltName: Full=P2;
DE   Contains:
DE     RecName: Full=Protein X3;
DE   Contains:
DE     RecName: Full=Protein X4;
DE   Contains:
DE     RecName: Full=Movement protein;
DE              Short=MP;
DE   Contains:
DE     RecName: Full=Coat protein;
DE              Short=CP;
OS   Tomato ringspot virus (isolate raspberry) (ToRSV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Picornavirales; Secoviridae; Comovirinae; Nepovirus.
OX   NCBI_TaxID=12281;
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH   NCBI_TaxID=4030; Pelargonium.
OH   NCBI_TaxID=3754; Prunus.
OH   NCBI_TaxID=23216; Rubus (bramble).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1856689; DOI=10.1099/0022-1317-72-7-1505;
RA   Rott M.E., Tremaine J.H., Rochon D.M.;
RT   "Nucleotide sequence of tomato ringspot virus RNA-2.";
RL   J. Gen. Virol. 72:1505-1514(1991).
RN   [2]
RP   PROTEOLYTIC PROCESSING OF POLYPROTEIN.
RX   PubMed=9049338; DOI=10.1099/0022-1317-76-4-917;
RA   Hans F., Sanfacon H.;
RT   "Tomato ringspot nepovirus protease: characterization and cleavage site
RT   specificity.";
RL   J. Gen. Virol. 76:917-927(1995).
RN   [3]
RP   PROTEOLYTIC PROCESSING OF POLYPROTEIN, AND MUTAGENESIS OF GLN-301 AND
RP   GLN-319.
RX   PubMed=11413391; DOI=10.1099/0022-1317-82-7-1785;
RA   Carrier K., Xiang Y., Sanfacon H.;
RT   "Genomic organization of RNA2 of Tomato ringspot virus: processing at a
RT   third cleavage site in the N-terminal region of the polyprotein in vitro.";
RL   J. Gen. Virol. 82:1785-1790(2001).
CC   -!- FUNCTION: Protein 2A: implicated in RNA2 replication. Could also be
CC       required for nematode transmission of the virus (By similarity).
CC       {ECO:0000250}.
CC   -!- FUNCTION: [Movement protein]: Transports viral genome to neighboring
CC       plant cells directly through plasmosdesmata, without any budding. The
CC       movement protein allows efficient cell to cell propagation, by
CC       bypassing the host cell wall barrier. Acts by forming a tubular
CC       structure at the host plasmodesmata, enlarging it enough to allow free
CC       passage of virion capsids (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cell junction, host plasmodesma.
CC       Note=Assembles in tubules that are embedded within modified
CC       plasmodesmata (By similarity). Movement proteins are targeted
CC       preferentially to calreticulin-labeled foci within the youngest cross
CC       walls, where they assemble into tubules. During cell division, they
CC       colocalize in the cell plate with KNOLLE, a cytokinesis-specific
CC       syntaxin (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [Coat protein]: Virion {ECO:0000305}.
CC   -!- PTM: Specific enzymatic cleavages in vivo by the P1 encoded 3C-like
CC       protease yield mature proteins. {ECO:0000269|PubMed:11413391,
CC       ECO:0000269|PubMed:9049338}.
CC   -!- MISCELLANEOUS: Virions are comprised of 60 copies of the coat protein.
CC   -!- SIMILARITY: Belongs to the nepoviruses RNA2 polyprotein family.
CC       {ECO:0000305}.
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DR   EMBL; D12477; BAA02043.1; -; Genomic_RNA.
DR   PIR; JQ1093; GNVVTR.
DR   RefSeq; NP_620762.1; NC_003839.2.
DR   SMR; P25247; -.
DR   PRIDE; P25247; -.
DR   GeneID; 956646; -.
DR   KEGG; vg:956646; -.
DR   Proteomes; UP000000410; Genome.
DR   GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.20; -; 2.
DR   InterPro; IPR005054; Nepo_coat.
DR   InterPro; IPR005305; Nepo_coat_C.
DR   InterPro; IPR005306; Nepo_coat_N.
DR   InterPro; IPR029053; Viral_coat.
DR   Pfam; PF03391; Nepo_coat; 1.
DR   Pfam; PF03688; Nepo_coat_C; 1.
DR   Pfam; PF03689; Nepo_coat_N; 1.
PE   1: Evidence at protein level;
KW   Capsid protein; Host cell junction; Reference proteome; Repeat; Transport;
KW   Viral movement protein; Virion.
FT   CHAIN           1..301
FT                   /note="Protein X3"
FT                   /id="PRO_0000037127"
FT   CHAIN           302..934
FT                   /note="Protein X4"
FT                   /id="PRO_0000037128"
FT   CHAIN           935..1320
FT                   /note="Movement protein"
FT                   /id="PRO_0000037129"
FT   CHAIN           1321..1882
FT                   /note="Coat protein"
FT                   /id="PRO_0000037130"
FT   REPEAT          554..606
FT                   /note="1"
FT   REPEAT          607..659
FT                   /note="2"
FT   REPEAT          660..698
FT                   /note="3; truncated and approximate"
FT   REGION          554..698
FT                   /note="2.5 X tandem repeats, Pro-rich"
FT   REGION          1289..1320
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1838..1863
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         301
FT                   /note="Q->A: No cleavage between X3 and X4."
FT                   /evidence="ECO:0000269|PubMed:11413391"
FT   MUTAGEN         319
FT                   /note="Q->A: No effect."
FT                   /evidence="ECO:0000269|PubMed:11413391"
SQ   SEQUENCE   1882 AA;  206803 MW;  0F8958B63AE8DD9D CRC64;
     MSSICFAGGN HARLPSKAAY YRAISDRELD REGRFPCGCL AQYTVQAPPP AKTQEKAVGR
     SADLQKGNVA PLKKQRCDVV VAVSGPPPLE LVYPARVGQH RLDQPSKGPL AVPSAKQTST
     AMEVVLSAEE AAITAPWLLR PCKGEAPPPP PLTQRQQFAA LKKRLAVKGQ QIIREHIRAR
     KAAKYAAIAK AKKAAALAAV KAAQEAPRLA AQKAAISKIL RDRDVAALPP PPPPSAARLA
     AEAELASKAE SLRRLKAFKT FSRVRPALNT SFPPPPPPPP ARSSELLAAF EAAMNRSQPV
     QGGFSLPTRK GVYVAPTVQG VVRAGLRAQK GFLNAVSTGI VAGARILKSK SQNWFRRSMG
     IAHDYVEGCM ASTVLGCAGP VVQRQEACSV VAAPPIVEPV LWVPPLSEYA NDFPKLTCST
     FTEWQRPRKQ SIAISNLFRK LIDRALLVSG VSLIASVLLF EIAENFAVRQ AVCPVEMPSC
     ATSVSEKSLV SLDEGNFYLR KYLSPPPYPF GRESFYFQAR PRFIGPMPSM VRAVPQIVQQ
     PTMTEELEFE VPSSWSSPLP LFANFKVNRG ACFLQVLPQR VVLPDECMDL LSLFEDQLPE
     GPLPSFSWSS PLPLFANFKV NRGACFLQVL PQRVVLPDEC MDLLSLFEDQ LPEGPLPSFS
     WSSPLPLFAS FKVNRGACFL QVLPARKVVS DEFMDVLPFL FSPLVSHQEE EPEMVPAVLE
     AADSVGDITE AFFDDLECES FYDSYSDEEE AEWAEVPRCK TMSELCASLT LAGDAEGLRK
     SHGVFLKRLV TYLQSFEEPL YSSRAFYSVK VKPVYRPKKF EGHIDCTCLD GNMGEWEWRE
     SVDAMWRCPG RLLNTKRTFT RDDWERVQYL RIGFNEGRYR RNWRVLNLEE MDLSLHEYPE
     ISSAPVQSSL FSRVVDRGAT LASSIPFVTR SNCQSSLGTP GLNVHTIHQE APTTLRAPPF
     TGARNVMGSS DAGANAAPYR SEARKRWLSR KQEDSQEDNI KRYADKHGIS FEEARAVYKA
     PKEGVPTQRS ILPDVRDAYS ARSAGARVRS LFGGSPTTRA QRTEDFVLTS PSAGDASSFS
     FYFNPVSEQE MAEQERGGNT MLSLDAVEVV IDPVGMPGDD TDLTVMVLWC QNSDDQRALI
     GAMSTFVGNG LARAVFYPGL KLLYANCRVR DGRVLKVIVS STNSTLTHGL PQAQVSIGTL
     RQHLGPGHDR TISGALYASQ QQGFNIRATE QGGAVTFAPQ GGHVEGIPSA NVQMGAGEHL
     IQAGPMQWRL QRSQSSRFVV SGHSRTRGSS LFTGSVDRTQ QGTGAFEDPG FLPPRNSSVQ
     GGSWQEGTEA AYLGKVTCAK DAKGGTLLHT LDIIKECKSQ NLLRYKEWQR QGFLHGKLRL
     RCFIPTNIFC GHSMMCSLDA FGRYDSNVLG ASFPVKLASL LPTEVISLAD GPVVTWTFDI
     GRLCGHGLYY SEGAYARPKI YFLVLSDNDV PAEADWQFTY QLLFEDHTFS NSFGAVPFIT
     LPHIFNRLDI GYWRGPTEID LTSTPAPNAY RLLFGLSTVI SGNMSTLNAN QALLRFFQGS
     NGTLHGRIKK IGTALTTCSL LLSLRHKDAS LTLETAYQRP HYILADGQGA FSLPISTPHA
     ATSFLEDMLR LEIFAIAGPF SPKDNKAKYQ FMCYFDHIEL VEGVPRTIAG EQQFNWCSFR
     NFKIDDWKFE WPARLPDILD DKSEVLLRQH PLSLLISSTG FFTGRAIFVF QWGLNTTAGN
     MKGSFSARLA FGKGVEEIEQ TSTVQPLVGA CEARIPVEFK TYTGYTTSGP PGSMEPYIYV
     RLTQAKLVDR LSVNVILQEG FSFYGPSVKH FKKEVGTPSA TLGTNNPVGR PPENVDTGGP
     GGQYAAALQA AQQAGKNPFG RG