ID   POL3_DROME              Reviewed;        1058 AA.
AC   P04323;
DT   20-MAR-1987, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-1987, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Retrovirus-related Pol polyprotein from transposon 17.6;
DE   Includes:
DE     RecName: Full=Protease;
DE              EC=3.4.23.-;
DE   Includes:
DE     RecName: Full=Reverse transcriptase;
DE              EC=2.7.7.49;
DE   Includes:
DE     RecName: Full=Endonuclease;
GN   Name=pol;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6209583; DOI=10.1038/312659a0;
RA   Saigo K., Kugimiya W., Matsuo Y., Inouye S., Yoshioka K., Yuki S.;
RT   "Identification of the coding sequence for a reverse transcriptase-like
RT   enzyme in a transposable genetic element in Drosophila melanogaster.";
RL   Nature 312:659-661(1984).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU00405};
CC   -!- MISCELLANEOUS: The open reading frame is located in a copia-like
CC       transposable element called 17.6.
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DR   EMBL; X01472; CAA25702.1; -; Genomic_DNA.
DR   PIR; A03971; GNFF17.
DR   AlphaFoldDB; P04323; -.
DR   SMR; P04323; -.
DR   MEROPS; A02.052; -.
DR   PRIDE; P04323; -.
DR   FlyBase; FBgn0014453; 17.6\pol.
DR   PRO; PR:P04323; -.
DR   GO; GO:0042575; C:DNA polymerase complex; IEA:UniProt.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProt.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0003964; F:RNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.70.10; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.30.70.270; -; 2.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR041588; Integrase_H2C2.
DR   InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR000477; RT_dom.
DR   InterPro; IPR041373; RT_RNaseH.
DR   Pfam; PF17921; Integrase_H2C2; 1.
DR   Pfam; PF17917; RT_RNaseH; 1.
DR   Pfam; PF00078; RVT_1; 1.
DR   SUPFAM; SSF50630; SSF50630; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00141; ASP_PROTEASE; 1.
DR   PROSITE; PS50994; INTEGRASE; 1.
DR   PROSITE; PS50878; RT_POL; 1.
PE   4: Predicted;
KW   Aspartyl protease; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotidyltransferase; Protease; RNA-directed DNA polymerase; Transferase;
KW   Transposable element.
FT   CHAIN           1..1058
FT                   /note="Retrovirus-related Pol polyprotein from transposon
FT                   17.6"
FT                   /id="PRO_0000199557"
FT   DOMAIN          231..415
FT                   /note="Reverse transcriptase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT   DOMAIN          810..967
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
FT   ACT_SITE        30
FT                   /note="For protease activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
SQ   SEQUENCE   1058 AA;  122698 MW;  C893F5C4A7E1F091 CRC64;
     TGRKFSATSL GKPQYITIKY KENNLKCLID TGSTVNMTSK NIFDLPIQNT STFIHTSNGP
     LIVNKSIIIP SKILFPTTNE FLLHPFSENY DLLLGRKLLA EAKATISYRD QEVTLYNNKY
     KLIEGIATHE QSHFQNVNMI PDTMLRQPNK ISPILESDLY RLEHLNNEEK QRLCALLQKY
     HDIQYHEGDK LTFTNQTKHT INTKHNLPLY SKYSYPQAYE QEVESQIQDM LNQGIIRTSN
     SPYNSPIWVV PKKQDASGKQ KFRIVIDYRK LNEITVGDRH PIPNMDEILG KLGRCNYFTT
     IDLAKGFHQI EMDPESVSKT AFSTKHGHYE YLRMPFGLKN APATFQRCMN DILRPLLNKH
     CLVYLDDIIV FSTSLDEHLQ SLGLVFEKLA KANLKLQLDK CEFLKQETTF LGHVLTPDGI
     KPNPEKIEAI QKYPIPTKPK EIKAFLGLTG YYRKFIPNFA DIAKPMTKCL KKNMKIDTTN
     PEYDSAFKKL KYLISEDPIL KVPDFTKKFT LTTDASDVAL GAVLSQDGHP LSYISRTLNE
     HEINYSTIEK ELLAIVWATK TFRHYLLGRH FEISSDHQPL SWLYRMKDPN SKLTRWRVKL
     SEFDFDIKYI KGKENCVADA LSRIKLEETY LSEQTQHSAE EDNSDLIFIT ERPLNTFNRQ
     VIFSKGPPDI KVTKYFKKHI TQIFYDIMTR EKAEQYLIDH FCGKKSALYI ESDADFEVIQ
     AAHKLAINTK YTKILRSTIL LKNITTYAEF KELILTAHEK LLHPGIQKTT KLFGETYYFP
     NSQLLIQNII NECSICNLAK TEHRNTDMPT KTTPKPEHCR EKFMIDIYSS EGKHYVSCID
     IYSKFATLEE IKTKDWIECK NALMRIFNQL GKPKLLKADR DGAFSSLALK RWLESEEVEL
     QLNTTKTGVA DIERLHKTIN EKIRIIKTSD DEETKLSKME TVLNIYNHKT KHDTTGQTPA
     HIFLYAGQPI LDTQQNKENK INKINNDRVE YEVDTRYRKG PLQKGKLENP FKPTKNVEQT
     DSDHYKITNR NRITHYYKTQ FKKRKKNNQL SISQAPGT