ID   POL5_DROME              Reviewed;        1003 AA.
AC   Q8I7P9;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Retrovirus-related Pol polyprotein from transposon opus;
DE   Includes:
DE     RecName: Full=Protease;
DE              EC=3.4.23.-;
DE   Includes:
DE     RecName: Full=Reverse transcriptase;
DE              EC=2.7.7.49;
DE   Includes:
DE     RecName: Full=Endonuclease;
GN   Name=pol;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227 {ECO:0000312|EMBL:AAN87271.1};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Berkeley {ECO:0000269|PubMed:12537573};
RX   PubMed=12537573; DOI=10.1186/gb-2002-3-12-research0084;
RA   Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J.W., Svirskas R.,
RA   Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M.,
RA   Celniker S.E.;
RT   "The transposable elements of the Drosophila melanogaster euchromatin: a
RT   genomics perspective.";
RL   Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU00405};
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DR   EMBL; AY180918; AAN87271.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8I7P9; -.
DR   SMR; Q8I7P9; -.
DR   FlyBase; FBgn0025875; opus\pol.
DR   PRO; PR:Q8I7P9; -.
DR   GO; GO:0042575; C:DNA polymerase complex; IEA:UniProt.
DR   GO; GO:0005634; C:nucleus; NAS:UniProtKB.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProt.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003964; F:RNA-directed DNA polymerase activity; NAS:UniProtKB.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0006313; P:transposition, DNA-mediated; NAS:UniProtKB.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.30.70.270; -; 2.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR041588; Integrase_H2C2.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR000477; RT_dom.
DR   InterPro; IPR041373; RT_RNaseH.
DR   Pfam; PF17921; Integrase_H2C2; 1.
DR   Pfam; PF17917; RT_RNaseH; 1.
DR   Pfam; PF00078; RVT_1; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50994; INTEGRASE; 1.
DR   PROSITE; PS50878; RT_POL; 1.
PE   4: Predicted;
KW   Endonuclease; Hydrolase; Nuclease; Nucleotidyltransferase; Protease;
KW   RNA-directed DNA polymerase; Transferase; Transposable element.
FT   CHAIN           1..1003
FT                   /note="Retrovirus-related Pol polyprotein from transposon
FT                   opus"
FT                   /id="PRO_0000199559"
FT   DOMAIN          147..331
FT                   /note="Reverse transcriptase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT   DOMAIN          752..910
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
SQ   SEQUENCE   1003 AA;  114635 MW;  C28AA23D6CEB8655 CRC64;
     MITHRLVGKF FKPLGNDSDI TFFVLPNLHS FDGIIGDDTL KDLKAIVDRK NNCLIITPGI
     KIPLLARASI NVNPLLAAEH PDGTQEILNS LLGEFPRIFE PPLSGMSVET AVKAEIRTNT
     QDPIYAKSYP YPVNMRGEVE RQIDELLQDG IIRPSNSPYN SPIWIVPKKP KPNGEKQYRM
     VVDFKRLNTV TIPDTYPIPD INATLASLGN AKYFTTLDLT SGFHQIHMKE SDIPKTAFST
     LNGKYEFLRL PFGLKNAPAI FQRMIDDILR EHIGKVCYVY IDDIIVFSED YDTHWKNLRL
     VLASLSKANL QVNLEKSHFL DTQVEFLGYI VTADGIKADP KKVRAISEMP PPTSVKELKR
     FLGMTSYYRK FIQDYAKVAK PLTNLTRGLY ANIKSSQSSK VPITLDETAL QSFNDLKSIL
     CSSEILAFPC FTKPFHLTTD ASNWAIGAVL SQDDQGRDRP IAYISRSLNK TEENYATIEK
     EMLAIIWSLD NLRAYLYGAG TIKVYTDHQP LTFALGNRNF NAKLKRWKAR IEEYNCELIY
     KPGKSNVVAD ALSRIPPQLN QLSTDLDANP EDDMQSLATA HSALHDSSRL IPHVESPINV
     FKNQLIFDTT RSKYLCEHPF PGYTRHLIPL KDGSLADLTN SLQSCLRPVI INGVKIPEAH
     LQRFQSICLA NFLLYKIRIT QRLVADVSGA EEICEIIEKE HRRAHRGPTE IRLQLLEKYY
     FPRMSSTIRL QTSSCQCCKL YKYERHPNKP NLQPTPIPNY PCEILHIDIF ALEKRLYLSC
     IDKFSKFAKL FHLQSKASVH LRETLVEALH YFTAPKVLVS DNERGLLCPT VLNYLRSLDI
     DLYYAPTQKS EVNGQVERFH STFLEIYRCL KDELPTFKPV ELVHIAVDRY NTSVHSVTNR
     KPADVFFDRS SRVNYQGLTD FRRQTLEDIK GLIEYKQIRG NMARNKNRDE PKSYGPGDEV
     FVANKQIKTK EKARFRCEKV QEDNKITVKT RSGKIFHKSD LRN