POLAR_ARATH
ID POLAR_ARATH Reviewed; 344 AA.
AC Q6NQ99;
DT 11-JUN-2014, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Protein POLAR LOCALIZATION DURING ASYMMETRIC DIVISION AND REDISTRIBUTION {ECO:0000303|PubMed:21963668};
GN Name=POLAR {ECO:0000303|PubMed:21963668};
GN OrderedLocusNames=At4g31805 {ECO:0000312|Araport:AT4G31805};
GN ORFNames=F28M20 {ECO:0000312|EMBL:AL031004};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR
RP LOCATION, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia; TISSUE=Seedling;
RX PubMed=21963668; DOI=10.1105/tpc.111.088583;
RA Pillitteri L.J., Peterson K.M., Horst R.J., Torii K.U.;
RT "Molecular profiling of stomatal meristemoids reveals new component of
RT asymmetric cell division and commonalities among stem cell populations in
RT Arabidopsis.";
RL Plant Cell 23:3260-3275(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP REVIEW.
RX PubMed=23044038; DOI=10.1016/j.pbi.2012.09.013;
RA Facette M.R., Smith L.G.;
RT "Division polarity in developing stomata.";
RL Curr. Opin. Plant Biol. 15:585-592(2012).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, PHOSPHORYLATION AT THR-19; SER-79; THR-84;
RP THR-86; SER-91; SER-94; THR-193; THR-217; THR-233; SER-235; SER-308;
RP SER-309; SER-320; SER-321 AND SER-336, MUTAGENESIS OF THR-19; SER-29;
RP SER-33; SER-79; THR-84; THR-86; SER-91; SER-94; THR-141; SER-145; SER-149;
RP SER-159; SER-163; THR-193; SER-197; THR-217; THR-233; SER-235; SER-308;
RP SER-309; SER-320; SER-324; SER-327; SER-331; TYR-332; SER-336 AND THR-340,
RP INTERACTION WITH BASL; ASK7/BIN2 AND ASK3/SK12, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=30429609; DOI=10.1038/s41586-018-0714-x;
RA Houbaert A., Zhang C., Tiwari M., Wang K., de Marcos Serrano A.,
RA Savatin D.V., Urs M.J., Zhiponova M.K., Gudesblat G.E., Vanhoutte I.,
RA Eeckhout D., Boeren S., Karimi M., Betti C., Jacobs T., Fenoll C., Mena M.,
RA de Vries S., De Jaeger G., Russinova E.;
RT "POLAR-guided signalling complex assembly and localization drive asymmetric
RT cell division.";
RL Nature 563:574-578(2018).
CC -!- FUNCTION: Regulates asymmetric cell division (ACD), especially in
CC stomatal-lineage cells (PubMed:21963668). Acts as a stomatal lineage
CC scaffold which regulates subcellular localization and transient
CC polarization of kinases (e.g. ASK7/BIN2 and ASK3/SK12) involved in ACD
CC in a BASL-dependent manner (PubMed:30429609). Promotes the
CC differentiation of both pavement cells and stomata (PubMed:30429609).
CC {ECO:0000269|PubMed:21963668, ECO:0000269|PubMed:30429609}.
CC -!- SUBUNIT: Component of a complex made of POLAR, BASL, ASK7/BIN2 and
CC ASK3/SK12 (PubMed:30429609). Interacts with BASL, ASK7/BIN2 and
CC ASK3/SK12 (PubMed:30429609). {ECO:0000269|PubMed:30429609}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21963668,
CC ECO:0000269|PubMed:30429609}. Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:21963668, ECO:0000269|PubMed:30429609}.
CC Note=Localized throughout the plasma membrane in asymmetric cell
CC division (ACD) precursors (PubMed:30429609). Transient polar
CC localization and segregation unevenly (distal to the newly divided
CC meristemoid and parallel to the division plane) during meristemoid
CC asymmetric divisions in a BASL-dependent manner (PubMed:21963668,
CC PubMed:30429609). Present in the cytoplasm of meristemoids and cell
CC periphery in stomatal lineage ground cells (SLGCs), in a polarized
CC pattern (PubMed:21963668, PubMed:30429609).
CC {ECO:0000269|PubMed:21963668, ECO:0000269|PubMed:30429609}.
CC -!- TISSUE SPECIFICITY: Expressed in stomatal lineage cells with asymmetric
CC division potential. {ECO:0000269|PubMed:21963668}.
CC -!- DEVELOPMENTAL STAGE: During germination, first observed in a subset of
CC protodermal cells, likely meristemoid mother cells (MMCs), both in the
CC cytosol and at the cell periphery (PubMed:21963668, PubMed:30429609).
CC Two hours before each asymmetric division occurs, becomes dynamically
CC localized at the cell cortex distal to the division plane, especially
CC in cells of the stomatal lineage. After asymmetric cell division, up-
CC regulated in only one of the daughter cells that will continue to
CC divide asymmetrically (PubMed:21963668). {ECO:0000269|PubMed:21963668,
CC ECO:0000269|PubMed:30429609}.
CC -!- PTM: Phosphorylation by ASK7/BIN2 is increases turnover.
CC {ECO:0000269|PubMed:30429609}.
CC -!- DISRUPTION PHENOTYPE: No visible effect (PubMed:21963668). Decreased
CC accumulation of ASK7/BIN2 at the plasma membrane and impaired
CC polarization of ASK7/BIN2 in asymmetric cell division (ACD) precursors
CC thus leading to its nuclear localization in the meristemoids
CC (PubMed:30429609). {ECO:0000269|PubMed:21963668,
CC ECO:0000269|PubMed:30429609}.
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DR EMBL; JN663804; AEQ76834.1; -; mRNA.
DR EMBL; AL031004; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002687; AEE85962.1; -; Genomic_DNA.
DR EMBL; BT010559; AAQ65182.1; -; mRNA.
DR EMBL; AK227333; BAE99346.1; -; mRNA.
DR RefSeq; NP_567883.1; NM_119330.3.
DR AlphaFoldDB; Q6NQ99; -.
DR SMR; Q6NQ99; -.
DR STRING; 3702.AT4G31805.1; -.
DR iPTMnet; Q6NQ99; -.
DR PaxDb; Q6NQ99; -.
DR PRIDE; Q6NQ99; -.
DR ProteomicsDB; 236578; -.
DR EnsemblPlants; AT4G31805.1; AT4G31805.1; AT4G31805.
DR GeneID; 829309; -.
DR Gramene; AT4G31805.1; AT4G31805.1; AT4G31805.
DR KEGG; ath:AT4G31805; -.
DR Araport; AT4G31805; -.
DR TAIR; locus:505006539; AT4G31805.
DR eggNOG; ENOG502RZ5T; Eukaryota.
DR HOGENOM; CLU_063347_1_0_1; -.
DR InParanoid; Q6NQ99; -.
DR OMA; VSWWKET; -.
DR OrthoDB; 1373738at2759; -.
DR PhylomeDB; Q6NQ99; -.
DR PRO; PR:Q6NQ99; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q6NQ99; baseline and differential.
DR Genevisible; Q6NQ99; AT.
DR GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR GO; GO:0071944; C:cell periphery; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0008356; P:asymmetric cell division; IGI:UniProtKB.
DR InterPro; IPR040348; POLAR-like.
DR PANTHER; PTHR33476; PTHR33476; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..344
FT /note="Protein POLAR LOCALIZATION DURING ASYMMETRIC
FT DIVISION AND REDISTRIBUTION"
FT /id="PRO_0000429316"
FT COILED 262..297
FT /evidence="ECO:0000255"
FT MOD_RES 19
FT /note="Phosphothreonine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 79
FT /note="Phosphoserine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 84
FT /note="Phosphothreonine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 86
FT /note="Phosphothreonine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 91
FT /note="Phosphoserine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 94
FT /note="Phosphoserine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 193
FT /note="Phosphothreonine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 217
FT /note="Phosphothreonine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 233
FT /note="Phosphothreonine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 235
FT /note="Phosphoserine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 308
FT /note="Phosphoserine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 309
FT /note="Phosphoserine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 320
FT /note="Phosphoserine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 321
FT /note="Phosphoserine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MOD_RES 336
FT /note="Phosphoserine; by ASK7"
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 19
FT /note="T->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-29, A-33, A-79, A-84,
FT A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 29
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-33, A-79, A-84,
FT A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 33
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-79, A-84,
FT A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 79
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-84,
FT A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 84
FT /note="T->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 86
FT /note="T->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-91, A-94, A-141, A-145, A-149, A-159, A-163, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 91
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-94, A-141, A-145, A-149, A-159, A-163, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 94
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-141, A-145, A-149, A-159, A-163, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 141
FT /note="T->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-145, A-149, A-159, A-163, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 145
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-149, A-159, A-163, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 149
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-159, A-163, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 159
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-163, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 163
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-193,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 193
FT /note="T->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 197
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-217, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 217
FT /note="T->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-197, A-233, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 233
FT /note="T->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-197, A-217, A-235, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 235
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-197, A-217, A-233, A-308, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 308
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-197, A-217, A-233, A-235, A-309, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 309
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-197, A-217, A-233, A-235, A-308, A-320, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 320
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-197, A-217, A-233, A-235, A-308, A-309, A-324, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 324
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-
FT 327, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 327
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-
FT 324, A-331, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 331
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-
FT 324, A-327, A-332, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 332
FT /note="Y->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-
FT 324, A-327, A-331, A-336 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 336
FT /note="S->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-
FT 324, A-327, A-331, A-332 and A-340."
FT /evidence="ECO:0000269|PubMed:30429609"
FT MUTAGEN 340
FT /note="T->A: Reduced ASK7-mediated phosphorylation,
FT enhanced stabilization at the plasma membrane and increased
FT cell division; when associated with A-19, A-29, A-33, A-79,
FT A-84, A-86, A-91, A-94, A-141, A-145, A-149, A-159, A-163,
FT A-193, A-197, A-217, A-233, A-235, A-308, A-309, A-320, A-
FT 324, A-327, A-331, A-332 and A-336."
FT /evidence="ECO:0000269|PubMed:30429609"
SQ SEQUENCE 344 AA; 39765 MW; B1302BDD737465B1 CRC64;
MEGSRRRRGD GCTIVQCYTP RRFVGRWLSG LRSSKGKRDA GEEQEDDTRR YQLAPIRCST
SLNQLVMQDN KQNCRLNKSK EPETNTFESQ SRESPLEVGI GSFLLYLVVA SKTELDKMTN
LRMQMEMFLL NAKEQLQKKD TPMSSNEASG FQFSPQEFSN LASSIFQESS SSVLQEEYTE
FEVSEPEDYR RGTDCNSKLQ AEVGRLPLGE KAEDRQTKHQ IQRQCKLKDN EVTKSHIPEM
VVSDERYGVC PYELDKKLHE LLETRQQEEL VKLETALNRV ERRLQEKETE VSWWKDAARL
LAQRVPESSR SGLEWCNPDS STCSERSVPR SYEACSIHRT SFSR
