ID   POLA_CHPVU              Reviewed;         622 AA.
AC   Q9YTU3;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Polyprotein p69;
DE   AltName: Full=ORFA polyprotein;
DE   Contains:
DE     RecName: Full=Papain-like protease p29 {ECO:0000255|PROSITE-ProRule:PRU01225};
DE              EC=3.4.22.- {ECO:0000255|PROSITE-ProRule:PRU01225};
DE   Contains:
DE     RecName: Full=p40 protein;
OS   Cryphonectria hypovirus 1 (strain Euro7) (CHV-1/Euro7) (Chestnut blight
OS   fungus hypovirulence-associated virus).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes;
OC   Durnavirales; Hypoviridae; Hypovirus.
OX   NCBI_TaxID=321609;
OH   NCBI_TaxID=5116; Cryphonectria parasitica (Chestnut blight fungus) (Endothia parasitica).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=9882299; DOI=10.1128/jvi.73.2.985-992.1999;
RA   Chen B., Nuss D.L.;
RT   "Infectious cDNA clone of hypovirus CHV1-Euro7: a comparative virology
RT   approach to investigate virus-mediated hypovirulence of the chestnut blight
RT   fungus Cryphonectria parasitica.";
RL   J. Virol. 73:985-992(1999).
CC   -!- FUNCTION: P40 protein is involved in reduction of conidiation of the
CC       host. Not necessary for replication. Also involved in reduction of
CC       orange pigmentation of the host (By similarity). {ECO:0000250}.
CC   -!- FUNCTION: [Papain-like protease p29]: Cysteine protease of the
CC       peptidase family C7 that contributes to hypovirulence-associated traits
CC       like the reduction in conidiation and laccase activity, but not to
CC       virulence attenuation. Acts as suppressor of RNA-mediated gene
CC       silencing, also known as post-transcriptional gene silencing (PTGS), a
CC       mechanism of viral defense that limits the accumulation of viral RNAs.
CC       Enhances viral dsRNA accumulation and virus transmission. Also involved
CC       in the reduction in orange pigmentation of the host, an effect
CC       independent of the intrinsic protease activity. {ECO:0000255|PROSITE-
CC       ProRule:PRU01225}.
CC   -!- PTM: [Papain-like protease p29]: Autocatalytically processed.
CC       {ECO:0000250|UniProtKB:P10941}.
CC   -!- MISCELLANEOUS: Hypoviruses induce hypovirulence in their fungal host
CC       Cryphonectria parasitica. The consequence is attenuation of the related
CC       fungal disease, chestnut blight, that causes cankers that enlarge and
CC       kill branches and trunks. The virus-like genetic elements consist of
CC       cytoplasmically replicating double-stranded RNA.
CC   -!- MISCELLANEOUS: CHV-1 strain Euro7 is a mild hypovirulent strain.
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DR   EMBL; AF082191; AAD13749.1; -; Genomic_RNA.
DR   Proteomes; UP000008451; Genome.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR002704; Peptidase_C7_dom.
DR   Pfam; PF01830; Peptidase_C7; 1.
DR   PROSITE; PS51877; HAV_P29_PRO; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Suppressor of RNA silencing; Thiol protease.
FT   CHAIN           1..248
FT                   /note="Papain-like protease p29"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000038879"
FT   CHAIN           249..622
FT                   /note="p40 protein"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000038880"
FT   DOMAIN          1..248
FT                   /note="Peptidase C7"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01225"
FT   ACT_SITE        162
FT                   /note="For papain-like protease p29 activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01225"
FT   ACT_SITE        215
FT                   /note="For papain-like protease p29 activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01225"
FT   SITE            248..249
FT                   /note="Cleavage; by papain-like protease p29"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01225"
SQ   SEQUENCE   622 AA;  69993 MW;  56500B4E5F6EB1DF CRC64;
     MSCLRKPSQS LVLSESVDPT TVDPFVDVRA EEVVPTGCMT LWEYRDSCGD VPGPLSHGDL
     RRLRTPDGVC KCQIHFEFPT VLKSGSTGTV PEHPAVVAAF MGRPRRCSLE QRTKELDFRF
     LQLVHEGLPV RPSYMIARPP RPVRGLCSSR DGSLAQFGQG YCYLSAIVDS ARWRVARTTG
     WCVRVADYLR LLQWVGRRSF GSFQIEESTV EHVYHVIVDT EHQSEQDGAL FYQAVSDLAA
     RDPLARIGNQ LNPLAAEFCP RSARRVEPVT PQVTRRKGLA RMPGRSPTVV SVGNVGMAIT
     SIQDALVATE LRNVNFGRRD TEAECRRLWA RYEVNDYFRR HKAELLKFDA RLRSHMAKKP
     VSVRTRSSDA KIQCIGWRDR HLLPQRLAGL TKQKRSLIWS RFATSNIRRK ASACTMTPSV
     DPMVHTWGDS AALAAKKISE ARRRQEICAA AYAERAKARG QTNVVASISE AIETTLRRNG
     TRFALDGLHV AASVIVTTRL RSWNQEEVLA GREFRKSTTS WIWRHMPSSI QDALNLTSVR
     DKLDPGRAFG YVQAAVAQGM SDFRRAKRSL AIVAKPVIRN VRDPYDHGFV KRDGKLRHSR
     DVSNKKLRTK AVAATKVHKI IF