ID   POLC1_BRACM             Reviewed;          79 AA.
AC   P69197; Q42470;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Polcalcin Bra r 1;
DE   AltName: Full=Calcium-binding pollen allergen Bra r 1;
DE   AltName: Allergen=Bra r 1;
OS   Brassica campestris (Field mustard).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3711;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. S9; TISSUE=Pollen;
RX   PubMed=8616215; DOI=10.1007/bf00020459;
RA   Toriyama K., Okada T., Watanabe M., Ide T., Ashida T., Xu H., Singh M.;
RT   "A cDNA clone encoding an IgE-binding protein from Brassica anther has
RT   significant sequence similarity to Ca(2+)-binding proteins.";
RL   Plant Mol. Biol. 29:1157-1165(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10945346; DOI=10.1093/pcp/41.6.757;
RA   Okada T., Sasaki Y., Ohta R., Onozuka N., Toriyama K.;
RT   "Expression of Bra r 1 gene in transgenic tobacco and Bra r 1 promoter
RT   activity in pollen of various plant species.";
RL   Plant Cell Physiol. 41:757-766(2000).
CC   -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE.
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DR   EMBL; D63153; BAA09634.1; -; mRNA.
DR   EMBL; AB032260; BAA93509.1; -; Genomic_DNA.
DR   PIR; S65151; S65151.
DR   RefSeq; XP_009126190.1; XM_009127942.2.
DR   AlphaFoldDB; P69197; -.
DR   SMR; P69197; -.
DR   STRING; 3711.Bra023621.1-P; -.
DR   Allergome; 10125; Bra r 5.0101.
DR   Allergome; 171; Bra r 5.
DR   EnsemblPlants; Bra023621.1; Bra023621.1-P; Bra023621.
DR   GeneID; 103851111; -.
DR   Gramene; Bra023621.1; Bra023621.1-P; Bra023621.
DR   KEGG; brp:103851111; -.
DR   OMA; INFARAN; -.
DR   OrthoDB; 1559869at2759; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:EnsemblPlants.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   CDD; cd00051; EFh; 1.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF13499; EF-hand_7; 1.
DR   SMART; SM00054; EFh; 2.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS00018; EF_HAND_1; 2.
DR   PROSITE; PS50222; EF_HAND_2; 2.
PE   1: Evidence at protein level;
KW   Allergen; Calcium; Metal-binding; Repeat.
FT   CHAIN           1..79
FT                   /note="Polcalcin Bra r 1"
FT                   /id="PRO_0000073668"
FT   DOMAIN          1..36
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          39..71
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         14
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         16
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         18
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         20
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         25
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         49
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         51
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         53
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         55
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         60
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ   SEQUENCE   79 AA;  8644 MW;  313D4F954521D6B6 CRC64;
     MADAEHERIF KKFDTDGDGK ISAAELEEAL KKLGSVTPDD VTRMMAKIDT DGDGNISFQE
     FTEFASANPG LMKDVAKVF