ID   POLC4_ALNGL             Reviewed;          85 AA.
AC   O81701;
DT   20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Polcalcin Aln g 4;
DE   AltName: Full=Calcium-binding pollen allergen Aln g 4;
DE   AltName: Allergen=Aln g 4;
OS   Alnus glutinosa (European alder) (Betula alnus var. glutinosa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fagales; Betulaceae; Alnus.
OX   NCBI_TaxID=3517;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, AND MASS SPECTROMETRY.
RC   TISSUE=Pollen;
RX   PubMed=9862740;
RA   Hayek B., Vangelista L., Pastore A., Sperr W.R., Valent P., Vrtala S.,
RA   Niederberger V., Twardosz A., Kraft D., Valenta R.;
RT   "Molecular and immunologic characterization of a highly cross-reactive two
RT   EF-hand calcium-binding alder pollen allergen, Aln g 4: structural basis
RT   for calcium-modulated IgE recognition.";
RL   J. Immunol. 161:7031-7039(1998).
CC   -!- MASS SPECTROMETRY: Mass=9318.6; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:9862740};
CC   -!- ALLERGEN: Causes an allergic reaction in human.
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DR   EMBL; Y17713; CAA76831.1; -; mRNA.
DR   AlphaFoldDB; O81701; -.
DR   SMR; O81701; -.
DR   Allergome; 3056; Aln g 4.0101.
DR   Allergome; 9; Aln g 4.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   CDD; cd00051; EFh; 1.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF13499; EF-hand_7; 1.
DR   SMART; SM00054; EFh; 2.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS00018; EF_HAND_1; 2.
DR   PROSITE; PS50222; EF_HAND_2; 2.
PE   1: Evidence at protein level;
KW   Allergen; Calcium; Metal-binding; Repeat.
FT   CHAIN           1..85
FT                   /note="Polcalcin Aln g 4"
FT                   /id="PRO_0000073663"
FT   DOMAIN          7..42
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          45..77
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         20
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         22
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         24
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         26
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         31
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         55
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         57
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         59
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         66
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ   SEQUENCE   85 AA;  9362 MW;  903774CC1F714FAD CRC64;
     MADDHPQDQA EHERIFKCFD ANGDGKISAS ELGDALKTLG SVTPDEVKHM MAEIDTDGDG
     FISFQEFTNF ARANRGLVKD VAKIF