POLC4_BETPN
ID POLC4_BETPN Reviewed; 85 AA.
AC Q39419; O04131;
DT 20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Polcalcin Bet v 4;
DE AltName: Full=Calcium-binding pollen allergen Bet v 4;
DE AltName: Allergen=Bet v 4;
GN Name=BETV4;
OS Betula pendula (European white birch) (Betula verrucosa).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fagales; Betulaceae; Betula.
OX NCBI_TaxID=3505;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Pollen;
RX PubMed=9353329; DOI=10.1074/jbc.272.45.28630;
RA Engel E., Richter K., Obermeyer G., Briza P., Kungl A.J., Simon B.,
RA Auer M., Ebner C., Rheinberger H.J., Breitenbach M., Ferreira F.;
RT "Immunological and biological properties of Bet v 4, a novel birch pollen
RT allergen with two EF-hand calcium-binding domains.";
RL J. Biol. Chem. 272:28630-28637(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Pollen;
RX PubMed=9345295; DOI=10.1006/bbrc.1997.6860;
RA Twardosz A., Hayek B., Seiberler S., Pastore A., Vangelista L.,
RA Groenlund H., Kraft D., Valenta R.;
RT "Molecular characterization, expression in Escherichia coli, and epitope
RT analysis of a two EF-hand calcium-binding birch pollen allergen, Bet v 4.";
RL Biochem. Biophys. Res. Commun. 239:197-204(1997).
RN [3]
RP STRUCTURE BY NMR OF 2-85, AND SUBUNIT.
RX PubMed=15037075; DOI=10.1016/j.jmb.2003.12.070;
RA Neudecker P., Nerkamp J., Eisenmann A., Nourse A., Lauber T., Schweimer K.,
RA Lehmann K., Schwarzinger S., Ferreira F., Rosch P.;
RT "Solution structure, dynamics, and hydrodynamics of the calcium-bound
RT cross-reactive birch pollen allergen Bet v 4 reveal a canonical monomeric
RT two EF-hand assembly with a regulatory function.";
RL J. Mol. Biol. 336:1141-1157(2004).
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:15037075}.
CC -!- ALLERGEN: Causes an allergic reaction in human.
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DR EMBL; X87153; CAA60628.1; -; mRNA.
DR EMBL; Y12560; CAA73147.1; -; mRNA.
DR PIR; JC5711; JC5711.
DR PIR; S54819; S54819.
DR PDB; 1H4B; NMR; -; A=2-85.
DR PDBsum; 1H4B; -.
DR AlphaFoldDB; Q39419; -.
DR SMR; Q39419; -.
DR MINT; Q39419; -.
DR Allergome; 129; Bet v 4.
DR Allergome; 3138; Bet v 4.0101.
DR EvolutionaryTrace; Q39419; -.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Calcium; Metal-binding; Repeat.
FT CHAIN 1..85
FT /note="Polcalcin Bet v 4"
FT /id="PRO_0000073666"
FT DOMAIN 7..42
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 42..77
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 20
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 22
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 24
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 26
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 31
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 55
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 57
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 59
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 66
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT CONFLICT 71
FT /note="G -> A (in Ref. 2; CAA73147)"
FT /evidence="ECO:0000305"
FT HELIX 6..19
FT /evidence="ECO:0007829|PDB:1H4B"
FT STRAND 24..27
FT /evidence="ECO:0007829|PDB:1H4B"
FT HELIX 29..36
FT /evidence="ECO:0007829|PDB:1H4B"
FT HELIX 37..39
FT /evidence="ECO:0007829|PDB:1H4B"
FT HELIX 44..54
FT /evidence="ECO:0007829|PDB:1H4B"
FT HELIX 64..73
FT /evidence="ECO:0007829|PDB:1H4B"
FT HELIX 75..84
FT /evidence="ECO:0007829|PDB:1H4B"
SQ SEQUENCE 85 AA; 9448 MW; E2FC882E5BB8ADFF CRC64;
MADDHPQDKA ERERIFKRFD ANGDGKISAA ELGEALKTLG SITPDEVKHM MAEIDTDGDG
FISFQEFTDF GRANRGLLKD VAKIF
