POLC4_HESAR
ID POLC4_HESAR Reviewed; 165 AA.
AC A0T2M3;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 57.
DE RecName: Full=Polcalcin Cup a 4 {ECO:0000305};
DE AltName: Full=Calcium-binding pollen allergen Cup a 4 {ECO:0000305};
DE AltName: Allergen=Cup a 4 {ECO:0000303|PubMed:20869950};
OS Hesperocyparis arizonica (Arizona cypress) (Cupressus arizonica).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Pinopsida; Pinidae; Conifers II; Cupressales; Cupressaceae;
OC Hesperocyparis.
OX NCBI_TaxID=49011 {ECO:0000312|EMBL:ABK78767.1};
RN [1] {ECO:0000312|EMBL:ACY01951.1}
RP NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, TISSUE SPECIFICITY, ALLERGEN, AND
RP CIRCULAR DICHROISM ANALYSIS.
RC TISSUE=Pollen {ECO:0000303|PubMed:20869950};
RX PubMed=20869950; DOI=10.1016/j.bbrc.2010.09.079;
RA Pico de Coana Y., Parody N., Fuertes M.A., Carnes J., Roncarolo D.,
RA Ariano R., Sastre J., Mistrello G., Alonso C.;
RT "Molecular cloning and characterization of Cup a 4, a new allergen from
RT Cupressus arizonica.";
RL Biochem. Biophys. Res. Commun. 401:451-457(2010).
CC -!- SUBUNIT: May exist as monomer and dimer. {ECO:0000269|PubMed:20869950}.
CC -!- TISSUE SPECIFICITY: Expressed in mature pollen grains.
CC {ECO:0000269|PubMed:20869950}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE in 9.6% of
CC 177 patients allergic to Arizona cypress pollen.
CC {ECO:0000269|PubMed:20869950}.
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DR EMBL; EF079864; ABK78767.1; -; mRNA.
DR EMBL; GU015025; ACY01951.1; -; mRNA.
DR AlphaFoldDB; A0T2M3; -.
DR SMR; A0T2M3; -.
DR Allergome; 3552; Cup a 4.
DR Allergome; 8443; Cup a 4.0101.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR CDD; cd00051; EFh; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 2.
DR SMART; SM00054; EFh; 4.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 4.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW Allergen; Calcium; Metal-binding; Repeat.
FT CHAIN 1..165
FT /note="Polcalcin Cup a 4"
FT /id="PRO_0000447004"
FT DOMAIN 22..57
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 58..86
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 91..126
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 127..162
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 35
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 37
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 39
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 41
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 46
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 71
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 73
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 75
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 77
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 82
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 104
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 106
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 108
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 110
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 115
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 140
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 142
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 144
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 151
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 165 AA; 18038 MW; E94801B88797EF34 CRC64;
MDEVPSSDES KSASSGKRVL EQSVHELEEV FKKFDANGDG KISGSELADI LRSMGSEVDE
AEVKAMMEEA DTDGDGYVSL QEFVDLNIKG ATVKDLKNAF KVFDRDCNGT ISPAELCETL
KSVGEPCTIE ESKNIIHNVD KNGDGLINVE EFQTMMTSEM TDKSK
