ID   POLG_CILVC              Reviewed;        2512 AA.
AC   Q1KZ59;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=RNA replication protein;
DE   Includes:
DE     RecName: Full=Methyltransferase;
DE              EC=2.1.1.-;
DE   Includes:
DE     RecName: Full=Helicase;
DE              EC=3.6.4.13;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
OS   Citrus leprosis virus C (isolate Citrus sinesis/Brazil/Cordeiropolis/2003)
OS   (CiLV-C).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Kitaviridae; Cilevirus.
OX   NCBI_TaxID=686950;
OH   NCBI_TaxID=2711; Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=16894213; DOI=10.1099/vir.0.82038-0;
RA   Locali-Fabris E.C., Freitas-Astua J., Souza A.A., Takita M.A.,
RA   Astua-Monge G., Antonioli-Luizon R., Rodrigues V., Targon M.L.,
RA   Machado M.A.;
RT   "Complete nucleotide sequence, genomic organization and phylogenetic
RT   analysis of Citrus leprosis virus cytoplasmic type.";
RL   J. Gen. Virol. 87:2721-2729(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Locali E.C., Freitas-Astua J., Souza A.A., Takita M.A., Astua-Monge G.,
RA   Antonioli-Luizon R., Rodrigues V., Targon M.L.P.N., Machado M.A.;
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA replication. The central part of this protein possibly
CC       functions as an ATP-binding helicase (Potential). {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
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DR   EMBL; DQ352194; ABC75821.1; -; Genomic_RNA.
DR   RefSeq; YP_654538.1; NC_008169.1.
DR   PRIDE; Q1KZ59; -.
DR   GeneID; 4155849; -.
DR   KEGG; vg:4155849; -.
DR   Proteomes; UP000001101; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR002588; Alphavirus-like_MT_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR003323; OTU_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   Pfam; PF01660; Vmethyltransf; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR   PROSITE; PS50802; OTU; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   4: Predicted;
KW   ATP-binding; Helicase; Hydrolase; Methyltransferase;
KW   Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase;
KW   Reference proteome; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication.
FT   CHAIN           1..2512
FT                   /note="RNA replication protein"
FT                   /id="PRO_5000141183"
FT   DOMAIN          156..367
FT                   /note="Alphavirus-like MT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT   DOMAIN          683..803
FT                   /note="OTU"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00139"
FT   DOMAIN          1520..1696
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          1697..1874
FT                   /note="(+)RNA virus helicase C-terminal"
FT   DOMAIN          2265..2378
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   2512 AA;  286515 MW;  10C7CEA268A61227 CRC64;
     MSTHSTVETL DVNRVRELLR SHREASSHCD EPRFTATHTK GRVVASSEKP PVSFTIANGQ
     IVSDSEVITS TEAKAAAILS VISSYPKEIR EQLNGRIERG FTDNPDVDEA ARCIHSARLH
     NITTTALRKK PLLVHENVSN DMERFLNEKF LGYKIRLTFS KNVAHNNAAA LRRVLRFYMR
     DKVGYRKDDD IPDGYHVKNK DVGASGMDVI ADELTDVHCC TPDLDFRDHI RLERLKKYIY
     SHVCPTSKDH GIICEGFREG STKYRCENIG QQCYIEAPTL TFVHSAYDIT AAGIVDCMIA
     ANAHHAIMCL HFPSAILSGS TSGKDDLLQY KWDIVVEDGI KYYKQKFLND TQASYVHRLD
     VYLDKFLTKV VLGSDKRFYM FEITEMFGSV AIIEVFRREK DFIAGSKLTF NIPRTEHPNT
     VTIHTWEYVT GYESLFKGRN GLKSGVMRPV SIEVPEEFFL SVFKYGMTVD SNKFVYDGLL
     KSGVGIAARR NIGGTTMVDP SFPIPVRKLQ TFIVTMYMLL YQEKWEATQG LVTMKMLADQ
     YRTRSSKNGI VRFLTNVFSP DKVGETQHPS STYDLGKLGF SHSEDNKFVC DRNLNTEERS
     RLSQFERLVQ WFREFSRVHR KCPIVVHDST HMLEYVLDIP DMVVQNIKSL RDDLTLSNLC
     DHDFDAYLPS HIEVNDAKCT KDLTVIQVPG DGNCLYYCFV KACLYRGISV CDLKSRLRDS
     PYFLEVAKLA RDAGEDEFLD SLERDGVYGN KFTLILISKT FNVNICVHLK GGRELITHFI
     SNKGSRFIHL QLEHSHYSLL VPCIKAGLID EHVLCHGALS MVVPTTHDYR RLVDLYKIYT
     RDGTIASYFN IFNNSYKGPF LNVYELGFME IASSLEIPSS SGNKFLITDV WLHHCIKALR
     VLDPHSNVIV LRSSNTSRIP DRYGVCDFTM DSEFHEESFC LSTTLSDVLN LGIYSQCMVV
     FSDLSRVPLK PFAPGFRTRT SVAERSNKIL LAWSALSSGG TAVFRVFRPE EVPESLNMLT
     TLFEDIRFFK PKAISTSIVD GYLLCSGKRS HPGSEFSITS EVRNHFYTVN VENFYKQTLE
     ESEVSNYVKD LCGLYAGGGF VKPTRKHSYN RSFVLDRSLI LSKLMEFSSS VSFGLFGRKF
     STYKLHVGCD TKLKFCNRSI EEILDSQCLH CKYSKYDFNS LRSVSDLASF ATRERAVVVE
     TFDDCGDYVS IISFFHKLYS MCFKDLRVVS DCFLNNVVLK SFLAYARCYS DVEISLCQLK
     GRLLVDITCR SSWYNGCEFG DFELVSCNRH DYDTAFVEVL IQHAMHNQRY NNETIVINSR
     ADAIRAGLSV RKFKPCGDVL DDLNKSVKYK PKFVTYNTES LVNSIKAIVG VGDETTKEPT
     DNSVVEKPVV VFEKVDSPNL DDRIKAVYEY RSYMGRELSH SNDVLEKTVS NLLRFTETRD
     PKRLNEMYFP NSSFLSDEMK LKDSVGIITC NGKILKNSEP IVQFDDISAV YDIVNGSVVD
     KSDYFKMHRG KTTKQVGGFA IYTSLVAHNQ VESILKAVDC VYASEKINDL SAISIDWVQA
     VAGAGKTTLL VETFLITDLV VCPTVENRDS IRLRIKRRYP DLDPKEVDCR VRTINGYLVD
     FSTKLAKVTL NENTRLLVDE AIMYHAGCLF VLCMIYNIRR MFCVGDKKQI PFVSRIDFKL
     NYEKLCDFVN TEARPLARTF RSPPDVTYRM QQIYGKSLKG LTIQCLSKNQ DTSPSVSKLV
     ITKNYRFGQN FIREVFEKDK IDFDGKNLRI LFFLREDMLS FYGNGGMIFT DCCSTIHQFQ
     GSDAEYVVVM RLTYAEKSIF MDERQCLVAL TRHTKRMVYV SVNEGTDVLT RWINMPVVES
     MLVPHLSLSG GGTTTPSRYV TYRSIPSVDL MKGDKCVRVG YHPRSDIILD KRDTLTAVLN
     KIADARPKGN LVVSSAVLDK FNQQRLKPLL KSIVGHSNIF CAGVNSNINS TVFEVMQLNA
     VDHVPNHFID PIFDDDVVRS ADIGYKPYRQ HDNHDPVLSD YGFDDKFAVI QNFLCTTFPN
     SCYVPNYMDA WITYNLDLDL AIDDIVINVI KFATIDRTYD CMIPRLSFCS PVVRKACLVE
     SLIAVQKRNR NVPQLSSEVS PYVMADQLFD SLRSLLDERY YQEVHYGPAE LAAWLNDQKG
     SVVDEVIGEY CIYSTAVERY QLITKNSPKP TLSDEAYMEF AAPQVVLHQT KDINAVFCVI
     WRGIKTVVQS MLRHHNNIFM FADMDPDSFA DLLTEKVSTK VQETFDSLEI DIKKYDKSQD
     LKVLLLECKL LRYFGVSEEL VIIWFKSHVE SIVKDRRSGL KFKVQVQRRS GDGGTFIGNT
     LFLIALCARN FDLRKLKLAV FSGDDSLLVG EKRDLQCDSQ NFSDLFNLDV KFFPNFKYYH
     FCSKFLIAVE DRWYFIPDPV KLCIRLARLD LVNWGHIEEY RISLKDTTKY YCDDSIVREL
     SKAVCDRYPV AVDPAEVFRV VCSIVSSKDE FRLLFEEPLA CLPEGNLLPV IN