ID   POLG_PVYCH              Reviewed;         327 AA.
AC   P21294;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Genome polyprotein;
DE   Contains:
DE     RecName: Full=Nuclear inclusion protein B;
DE              Short=NI-B;
DE              Short=NIB;
DE     AltName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Contains:
DE     RecName: Full=Capsid protein;
DE              Short=CP;
DE     AltName: Full=Coat protein;
DE   Flags: Fragment;
OS   Potato virus Y (strain Chinese) (PVY).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Stelpaviricetes;
OC   Patatavirales; Potyviridae; Potyvirus.
OX   NCBI_TaxID=12218;
OH   NCBI_TaxID=4071; Capsicum (peppers).
OH   NCBI_TaxID=4085; Nicotiana.
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2216735; DOI=10.1093/nar/18.18.5554;
RA   Zhou X.R., Fang R.X., Wang C.Q., Mang K.Q.;
RT   "cDNA sequence of the 3'-coding region of PVY genome (the Chinese
RT   isolate).";
RL   Nucleic Acids Res. 18:5554-5554(1990).
RN   [2]
RP   REVIEW.
RX   PubMed=11226583; DOI=10.1016/s0168-1702(01)00220-9;
RA   Urcuqui-Inchima S., Haenni A.L., Bernardi F.;
RT   "Potyvirus proteins: a wealth of functions.";
RL   Virus Res. 74:157-175(2001).
CC   -!- FUNCTION: [Nuclear inclusion protein B]: An RNA-dependent RNA
CC       polymerase that plays an essential role in the virus replication.
CC   -!- FUNCTION: [Capsid protein]: Involved in aphid transmission, cell-to-
CC       cell and systemis movement, encapsidation of the viral RNA and in the
CC       regulation of viral RNA amplification. {ECO:0000250|UniProtKB:P04517}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBCELLULAR LOCATION: [Capsid protein]: Virion {ECO:0000305}.
CC   -!- PTM: Genome polyprotein of potyviruses undergoes post-translational
CC       proteolytic processing by the main proteinase NIa-pro resulting in the
CC       production of at least ten individual proteins. The P1 proteinase and
CC       the HC-pro cleave only their respective C-termini autocatalytically.
CC       6K1 is essential for proper proteolytic separation of P3 from CI (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the potyviridae genome polyprotein family.
CC       {ECO:0000305}.
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DR   EMBL; X54058; CAA37993.1; -; Genomic_RNA.
DR   PIR; S11435; S11435.
DR   SMR; P21294; -.
DR   GO; GO:0030430; C:host cell cytoplasm; ISS:UniProtKB.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR001592; Poty_coat.
DR   Pfam; PF00767; Poty_coat; 1.
PE   3: Inferred from homology;
KW   Capsid protein; Nucleotidyltransferase; RNA-directed RNA polymerase;
KW   Transferase; Virion.
FT   CHAIN           <1..60
FT                   /note="Nuclear inclusion protein B"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000040395"
FT   CHAIN           1..327
FT                   /note="Genome polyprotein"
FT                   /id="PRO_0000420015"
FT   CHAIN           61..327
FT                   /note="Capsid protein"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000040396"
FT   REGION          65..102
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        78..102
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            60..61
FT                   /note="Cleavage; by NIa-pro"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   327 AA;  36868 MW;  8F8355E2DE6F2F18 CRC64;
     QQQPFATIAQ EGKAPYIASM ALRKLYMDRA VDEEELRAFT EMMVALDDEF EFDSYEVHHQ
     ANDTIDAVGD NKKDAKPEQG SIQSNPNKGK EKDVNAGTSG THTVPRIKAI TPKMRMPKSK
     GATVLNLEHL LEYAPQQIDI SNTRATQSQF DTWYEAVRMA YDIGETEMPT VMNGLMVWCI
     ENGTSPNVNG VWVMMDGNEQ VGYPLKPIVE NAKPTLRQIM AHFSDVAEAY IEMRNKKEPY
     MPRYGLIRNL RDVGLARYAF DFYEVTSRTP VRAREAHIQM KAAALKSAQP RLFGLDGGIS
     TQEENTERHT TEDVSPSMHT LLGVKNM