ID   POLG_TAMV               Reviewed;         279 AA.
AC   P22556;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Genome polyprotein;
DE   Contains:
DE     RecName: Full=Nuclear inclusion protein B;
DE              Short=NI-B;
DE              Short=NIB;
DE     AltName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Contains:
DE     RecName: Full=Capsid protein;
DE              Short=CP;
DE     AltName: Full=Coat protein;
DE   Flags: Fragment;
OS   Tamarillo mosaic virus.
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Stelpaviricetes;
OC   Patatavirales; Potyviridae; Potyvirus.
OX   NCBI_TaxID=12226;
OH   NCBI_TaxID=45843; Solanum betaceum (Tamarillo) (Cyphomandra betacea).
OH   NCBI_TaxID=4112; Solanum nigrum (Black nightshade).
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA], AND PROTEIN SEQUENCE OF 11-20.
RX   PubMed=2263491; DOI=10.1093/nar/18.23.7166;
RA   Eagles R.M., Gardner R.C., Forster R.L.S.;
RT   "Nucleotide sequence of the tamarillo mosaic virus coat protein gene.";
RL   Nucleic Acids Res. 18:7166-7166(1990).
RN   [2]
RP   REVIEW.
RX   PubMed=11226583; DOI=10.1016/s0168-1702(01)00220-9;
RA   Urcuqui-Inchima S., Haenni A.L., Bernardi F.;
RT   "Potyvirus proteins: a wealth of functions.";
RL   Virus Res. 74:157-175(2001).
CC   -!- FUNCTION: [Nuclear inclusion protein B]: An RNA-dependent RNA
CC       polymerase that plays an essential role in the virus replication.
CC   -!- FUNCTION: [Capsid protein]: Involved in aphid transmission, cell-to-
CC       cell and systemis movement, encapsidation of the viral RNA and in the
CC       regulation of viral RNA amplification. {ECO:0000250|UniProtKB:P04517}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBCELLULAR LOCATION: [Capsid protein]: Virion {ECO:0000305}.
CC   -!- PTM: Genome polyprotein of potyviruses undergoes post-translational
CC       proteolytic processing by the main proteinase NIa-pro resulting in the
CC       production of at least ten individual proteins. The P1 proteinase and
CC       the HC-pro cleave only their respective C-termini autocatalytically.
CC       6K1 is essential for proper proteolytic separation of P3 from CI (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the potyviridae genome polyprotein family.
CC       {ECO:0000305}.
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DR   EMBL; X54804; CAA38575.1; -; Genomic_RNA.
DR   PIR; S13093; S13093.
DR   SMR; P22556; -.
DR   GO; GO:0030430; C:host cell cytoplasm; ISS:UniProtKB.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR001592; Poty_coat.
DR   Pfam; PF00767; Poty_coat; 1.
PE   1: Evidence at protein level;
KW   Capsid protein; Direct protein sequencing; Nucleotidyltransferase;
KW   RNA-directed RNA polymerase; Transferase; Virion.
FT   CHAIN           <1..11
FT                   /note="Nuclear inclusion protein B"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000040461"
FT   CHAIN           1..279
FT                   /note="Genome polyprotein"
FT                   /id="PRO_0000420028"
FT   CHAIN           12..279
FT                   /note="Capsid protein"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000040462"
FT   SITE            11..12
FT                   /note="Cleavage; by NIa-pro"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   279 AA;  31403 MW;  70C117175A063A78 CRC64;
     VDEEDDIVYF QAGTLDAGEA TAQKAEGKKK EGEVSSGKAV VVKDKDVDLG TAGTHSVPRL
     KSMTSKLTLP MLKGKSRCNL DHLLSYKQTV DLSNARATHE QFQNWYDGVM ASYELEESSM
     EIILNGFMVW CIENGTSPDI NGVWTMMDDE EQISYPLKPM LDHAKPSLRQ IMRHFSALAE
     AYIEMRSREK PYMPRYGLQR NLRDQSLARY AFDFYEITAT TPVRAKEAHL QMKAAALKNS
     NTNMFGLDGN VTTSEEDTER HTATDVNRNM HHLLGVKGV