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POLK_CAEEL
ID   POLK_CAEEL              Reviewed;         596 AA.
AC   P34409; A8WFE8; A8WFE9;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   25-JAN-2012, sequence version 3.
DT   03-AUG-2022, entry version 139.
DE   RecName: Full=DNA polymerase kappa;
DE            EC=2.7.7.7;
GN   Name=polk-1; ORFNames=F22B7.6;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=7906398; DOI=10.1038/368032a0;
RA   Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA   Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA   Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA   Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA   Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA   Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA   Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA   Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA   Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA   Wilkinson-Sproat J., Wohldman P.;
RT   "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT   elegans.";
RL   Nature 368:32-38(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: DNA polymerase specifically involved in DNA repair. Plays an
CC       important role in translesion synthesis, where the normal high-fidelity
CC       DNA polymerases cannot proceed and DNA synthesis stalls. Depending on
CC       the context, it inserts the correct base, but causes frequent base
CC       transitions, transversions and frameshifts. Lacks 3'-5' proofreading
CC       exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate
CC       at abasic sites, but does not have lyase activity (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Divalent metal cations. Prefers Mg(2+), but can also use Mn(2+).
CC       {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=a;
CC         IsoId=P34409-1; Sequence=Displayed;
CC       Name=b;
CC         IsoId=P34409-2; Sequence=VSP_032729;
CC   -!- DOMAIN: The catalytic core consists of fingers, palm and thumb
CC       subdomains, but the fingers and thumb subdomains are much smaller than
CC       in high-fidelity polymerases; residues from five sequence motifs of the
CC       Y-family cluster around an active site cleft that can accommodate DNA
CC       and nucleotide substrates with relaxed geometric constraints, with
CC       consequently higher rates of misincorporation and low processivity.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-Y family. {ECO:0000305}.
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DR   EMBL; FO080222; CCD62142.1; -; Genomic_DNA.
DR   EMBL; FO080222; CCD62143.1; -; Genomic_DNA.
DR   PIR; S44637; S44637.
DR   RefSeq; NP_001122692.1; NM_001129220.1. [P34409-1]
DR   RefSeq; NP_001122693.1; NM_001129221.3.
DR   AlphaFoldDB; P34409; -.
DR   SMR; P34409; -.
DR   STRING; 6239.F22B7.6a; -.
DR   EPD; P34409; -.
DR   PaxDb; P34409; -.
DR   EnsemblMetazoa; F22B7.6a.1; F22B7.6a.1; WBGene00017696. [P34409-1]
DR   EnsemblMetazoa; F22B7.6b.1; F22B7.6b.1; WBGene00017696. [P34409-2]
DR   EnsemblMetazoa; F22B7.6b.2; F22B7.6b.2; WBGene00017696. [P34409-2]
DR   GeneID; 176209; -.
DR   KEGG; cel:CELE_F22B7.6; -.
DR   UCSC; F22B7.6b.2; c. elegans. [P34409-1]
DR   CTD; 176209; -.
DR   WormBase; F22B7.6a; CE41638; WBGene00017696; polk-1. [P34409-1]
DR   WormBase; F22B7.6b; CE41639; WBGene00017696; polk-1. [P34409-2]
DR   eggNOG; KOG2094; Eukaryota.
DR   GeneTree; ENSGT00940000156667; -.
DR   HOGENOM; CLU_012348_11_4_1; -.
DR   InParanoid; P34409; -.
DR   OMA; RTQFKGR; -.
DR   OrthoDB; 878533at2759; -.
DR   PhylomeDB; P34409; -.
DR   Reactome; R-CEL-5655862; Translesion synthesis by POLK.
DR   Reactome; R-CEL-5696397; Gap-filling DNA repair synthesis and ligation in GG-NER.
DR   Reactome; R-CEL-5696400; Dual Incision in GG-NER.
DR   Reactome; R-CEL-6782135; Dual incision in TC-NER.
DR   Reactome; R-CEL-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR   PRO; PR:P34409; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00017696; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0009792; P:embryo development ending in birth or egg hatching; IGI:WormBase.
DR   GO; GO:0042276; P:error-prone translesion synthesis; IMP:WormBase.
DR   GO; GO:0000003; P:reproduction; IGI:WormBase.
DR   GO; GO:0019985; P:translesion synthesis; IMP:WormBase.
DR   CDD; cd03586; PolY_Pol_IV_kappa; 1.
DR   Gene3D; 3.30.1490.100; -; 1.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR036775; DNA_pol_Y-fam_lit_finger_sf.
DR   InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR   InterPro; IPR022880; DNApol_IV.
DR   InterPro; IPR024728; PolY_HhH_motif.
DR   InterPro; IPR006642; Rad18_UBZ4.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR001126; UmuC.
DR   Pfam; PF00817; IMS; 1.
DR   Pfam; PF11799; IMS_C; 1.
DR   Pfam; PF11798; IMS_HHH; 1.
DR   SUPFAM; SSF100879; SSF100879; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50173; UMUC; 1.
DR   PROSITE; PS51908; ZF_UBZ4; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; DNA damage; DNA repair; DNA replication;
KW   DNA synthesis; DNA-directed DNA polymerase; Magnesium; Metal-binding;
KW   Mutator protein; Nucleotidyltransferase; Nucleus; Reference proteome;
KW   Schiff base; Transferase; Zinc; Zinc-finger.
FT   CHAIN           1..596
FT                   /note="DNA polymerase kappa"
FT                   /id="PRO_0000173996"
FT   DOMAIN          85..320
FT                   /note="UmuC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00216"
FT   ZN_FING         516..545
FT                   /note="UBZ4-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01256"
FT   REGION          559..584
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        181
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00216"
FT   BINDING         89
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00216"
FT   BINDING         180
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00216"
FT   BINDING         519
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01256"
FT   BINDING         522
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01256"
FT   BINDING         536
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01256"
FT   BINDING         540
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01256"
FT   SITE            94
FT                   /note="Substrate discrimination"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00216"
FT   VAR_SEQ         1..133
FT                   /note="Missing (in isoform b)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_032729"
SQ   SEQUENCE   596 AA;  67942 MW;  48946E0994339523 CRC64;
     MLTFNDNKAG MNGLDKEKIT KVIEENTSAS YSSFSKKQQS RIEEKVLEIK NRLQTATREE
     RQKSEILMEN LEMKLESSRD LSRDCVCIDM DAYFAAVEMR DNPALRTVPM AVGSSAMLST
     SNYLARRFGV RAGMPGFISN KLCPSLTIVP GNYPKYTKVS RQFSQIFMEY DSDVGMMSLD
     EAFIDLTDYV ASNTEKKTFK RHRFGGDCPC WLPRFDENEN TLEDLKIEES ICPKCEKSRK
     IYYDHVEFGT GREEAVREIR FRVEQLTGLT CSAGIASNFM LAKICSDLNK PNGQYVLEND
     KNAIMEFLKD LPIRKVGGIG RVCEAQLKAM DIQTVGDMNL KKNLYPLCFT PLSQESFLRT
     ALGLPGRPSE SDPRRKSISV ERTFSPTSDF NILLEEHQEI CRMLEEDVRK SGIVGGKTVT
     LKLKLSSFDV LTRSLTPSDV VKSLEDIQKF SLELLEKEKG KEIRLLGVRL SQLIFEEDEK
     KRSKTITEFW NEKKLQIQNL QGSENVDDDD VIMMDTRPCP ICGTDVENRL DVMNCHVDEC
     ILKVQNDDGP ELICVSVENK STQKPERPST KKRKLQEKRP KAKKMVTIDS FWKKSG
 
 
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