POLLU_LOTJA
ID POLLU_LOTJA Reviewed; 917 AA.
AC Q5H8A5;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 70.
DE RecName: Full=Ion channel POLLUX;
GN Name=POLLUX;
OS Lotus japonicus (Lotus corniculatus var. japonicus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; robinioid clade; Loteae; Lotus.
OX NCBI_TaxID=34305;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, SUBCELLULAR
RP LOCATION, INDUCTION, AND MUTAGENESIS OF SER-145; GLY-303; SER-322; GLY-530;
RP GLU-830 AND GLY-882.
RC STRAIN=cv. Gifu / B-129;
RX PubMed=15616514; DOI=10.1038/nature03237;
RA Imaizumi-Anraku H., Takeda N., Charpentier M., Perry J., Miwa H.,
RA Umehara Y., Kouchi H., Murakami Y., Mulder L., Vickers K., Pike J.,
RA Downie J.A., Wang T., Sato S., Asamizu E., Tabata S., Yoshikawa M.,
RA Murooka Y., Wu G.-J., Kawaguchi M., Kawasaki S., Parniske M., Hayashi M.;
RT "Plastid proteins crucial for symbiotic fungal and bacterial entry into
RT plant roots.";
RL Nature 433:527-531(2005).
RN [2]
RP FUNCTION.
RX PubMed=16903357; DOI=10.1094/mpmi-19-0914;
RA Miwa H., Sun J., Oldroyd G.E., Downie J.A.;
RT "Analysis of Nod-factor-induced calcium signaling in root hairs of
RT symbiotically defective mutants of Lotus japonicus.";
RL Mol. Plant Microbe Interact. 19:914-923(2006).
RN [3]
RP FUNCTION, SUBUNIT, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=19106374; DOI=10.1105/tpc.108.063255;
RA Charpentier M., Bredemeier R., Wanner G., Takeda N., Schleiff E.,
RA Parniske M.;
RT "Lotus japonicus CASTOR and POLLUX are ion channels essential for
RT perinuclear calcium spiking in legume root endosymbiosis.";
RL Plant Cell 20:3467-3479(2008).
CC -!- FUNCTION: Ion channel with permeability for potassium. Involved in
CC perinuclear calcium spiking but not in cytosolic calcium influx.
CC Required for early signal transduction events leading to endosymbiosis.
CC Acts early in a signal transduction chain leading from the perception
CC of Nod factor to the activation of calcium spiking. Also involved in
CC fungal entry into root epidermal cells during the establishment of the
CC arbuscular mycorrhizal symbiosis. {ECO:0000269|PubMed:16903357,
CC ECO:0000269|PubMed:19106374}.
CC -!- SUBUNIT: Homooligomer. {ECO:0000269|PubMed:19106374}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000269|PubMed:15616514,
CC ECO:0000269|PubMed:19106374}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15616514, ECO:0000269|PubMed:19106374}. Note=The
CC chloroplastic localization proposed by PubMed:15616514 is probably an
CC overexpression artifact.
CC -!- TISSUE SPECIFICITY: Mainly expressed in nodules. Also detected in
CC infected and uninfected roots, leaves, seed pods, and flower buds.
CC {ECO:0000269|PubMed:15616514, ECO:0000269|PubMed:19106374}.
CC -!- INDUCTION: Slightly repressed during the first 2 days after bacterial
CC or Nod factor treatment. {ECO:0000269|PubMed:15616514}.
CC -!- SIMILARITY: Belongs to the castor/pollux (TC 1.A.1.23) family.
CC {ECO:0000305}.
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DR EMBL; AB162158; BAD89022.1; -; mRNA.
DR EMBL; AB162017; BAD89020.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5H8A5; -.
DR SMR; Q5H8A5; -.
DR TCDB; 1.A.1.23.3; the voltage-gated ion channel (vic) superfamily.
DR OMA; NHADTEG; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR044849; CASTOR/POLLUX/SYM8-like.
DR InterPro; IPR010420; CASTOR/POLLUX/SYM8_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR31563; PTHR31563; 1.
DR Pfam; PF06241; Castor_Poll_mid; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Ion channel; Ion transport; Membrane; Nucleus; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..917
FT /note="Ion channel POLLUX"
FT /id="PRO_0000004683"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 413..435
FT /evidence="ECO:0000255"
FT COMPBIAS 7..33
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 41..72
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 81..136
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 145
FT /note="S->F: In pollux-4; no nodules formation or
FT arbuscular mycorrhizal symbiosis."
FT /evidence="ECO:0000269|PubMed:15616514"
FT MUTAGEN 303
FT /note="G->S: In pollux-1 /Ljsym23-1 and pollux-2 /Ljsym23-
FT 2; no nodules formation or arbuscular mycorrhizal
FT symbiosis."
FT /evidence="ECO:0000269|PubMed:15616514"
FT MUTAGEN 322
FT /note="S->F: In pollux-5; no nodules formation or
FT arbuscular mycorrhizal symbiosis."
FT /evidence="ECO:0000269|PubMed:15616514"
FT MUTAGEN 530
FT /note="G->E: In pollux-7; no nodules formation or
FT arbuscular mycorrhizal symbiosis."
FT /evidence="ECO:0000269|PubMed:15616514"
FT MUTAGEN 830
FT /note="E->K: In pollux-9; no nodules formation or
FT arbuscular mycorrhizal symbiosis."
FT /evidence="ECO:0000269|PubMed:15616514"
FT MUTAGEN 841..917
FT /note="Missing: In pollux-3 /Ljsym86-1; no nodules
FT formation or arbuscular mycorrhizal symbiosis."
FT MUTAGEN 882
FT /note="G->S: In pollux-10; no nodules formation or
FT arbuscular mycorrhizal symbiosis."
FT /evidence="ECO:0000269|PubMed:15616514"
SQ SEQUENCE 917 AA; 102062 MW; AFDA8379F25D09D4 CRC64;
MIPLPVAAAN SNSNSNSNSN DEESPNLSTV IKPPLKKTKT LLPPPSSSSS NRPLHLRVSI
DNNNNNNAPP PPADFSDHQW NYPSFLGTTT RKRRPSSVKP PSTSNLRFDT IPKTKTKTKT
NTNTNTNTNT NTNTNTDLPP PPVPSSSPVA RPQHHNHRSP PIFYLLIITC IIFVPYSSYL
QYKLAKLEDH KLHLCRQSQI HFSSGHGNGK ISIPIHDASF SYILSRKAAL YIVLFTLILP
FLLYKYLDYL PQIINFLRRT HNNKEDVPLK KRIAYMLDVF FSIYPYAKLL ALLFATLFLI
GFGGLALYAV TGGSLAEALW HSWTYVADSG NHAETQGTGQ RVVSVSISSG GMLIFAMMLG
LVSDAISEKV DSLRKGKCEV IERNHILILG WSDKLGSLLK QLAIANKSVG GGVIVVLAEK
EKEEMEMDIT KLEFDFMGTS VICRSGSPLI LADLKKVSVS KARAIIVLAS DENADQSDAR
ALRVVLSLTG VKEGLRGHVV VEMSDLDNEP LVKLVGGELI ETVVAHDVIG RLMIQCALQP
GLAQIWEDIL GFENAEFYIK RWPELDGLSF KDILISFPDA IPCGVKVAAD GGKIVINPDD
SYVMRDGDEV LVIAEDDDTY SPGSLPEVLK GFFPRIPDAP KYPEKILFCG WRRDIDDMIM
VLEAFLAPGS ELWMFNEVPE KEREKKLAAG GLDVFGLENI KLVHREGNAV IRRHLESLPL
ETFDSILILA DESVEDSVAH SDSRSLATLL LIRDIQSRRL PYKDTKSTSL RLSGFSHNSW
IREMQQASDK SIIISEILDS RTRNLVSVSR ISDYVLSNEL VSMALAMVAE DKQINRVLEE
LFAEQGNEMC IKPAEFYLFD QEELCFYDIM IRGRARQEII IGYRLANQER AIINPSEKLV
ARKWSLGDVF VVIASGD
