POLLU_ORYSJ
ID POLLU_ORYSJ Reviewed; 965 AA.
AC Q5N941; Q0JHD3; Q5N940;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Probable ion channel POLLUX;
DE Short=OsPOLLUX;
DE AltName: Full=Probable ion channel DMI1;
GN OrderedLocusNames=Os01g0870100, LOC_Os01g64980;
GN ORFNames=P0039A07.9, P0677H08.41;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18852152; DOI=10.1093/pcp/pcn153;
RA Banba M., Gutjahr C., Miyao A., Hirochika H., Paszkowski U., Kouchi H.,
RA Imaizumi-Anraku H.;
RT "Divergence of evolutionary ways among common sym genes: CASTOR and CCaMK
RT show functional conservation between two symbiosis systems and constitute
RT the root of a common signaling pathway.";
RL Plant Cell Physiol. 49:1659-1671(2008).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18978069; DOI=10.1104/pp.108.131540;
RA Chen C., Fan C., Gao M., Zhu H.;
RT "Antiquity and function of CASTOR and POLLUX, the twin ion channel-encoding
RT genes key to the evolution of root symbioses in plants.";
RL Plant Physiol. 149:306-317(2009).
CC -!- FUNCTION: Required for mycorrhizal symbiosis.
CC {ECO:0000269|PubMed:18852152, ECO:0000269|PubMed:18978069}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5N941-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5N941-2; Sequence=VSP_013298, VSP_013299;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems and panicles.
CC {ECO:0000269|PubMed:18978069}.
CC -!- INDUCTION: Not induced by mycorrhizal colonization.
CC {ECO:0000269|PubMed:18978069}.
CC -!- DISRUPTION PHENOTYPE: Defective in mycorrhizal symbiosis.
CC {ECO:0000269|PubMed:18852152, ECO:0000269|PubMed:18978069}.
CC -!- MISCELLANEOUS: Can restore nodulation but not rhizobial infection to a
CC Medicago truncatula dmi1 mutant, but cannot restore mycorrhizal or
CC rhizobial symbiosis in a Ljpollux mutant of Lotus japonicus.
CC -!- SIMILARITY: Belongs to the castor/pollux (TC 1.A.1.23) family.
CC {ECO:0000305}.
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DR EMBL; AP003235; BAD81710.1; -; Genomic_DNA.
DR EMBL; AP003235; BAD81711.1; -; Genomic_DNA.
DR EMBL; AP003286; BAD82015.1; -; Genomic_DNA.
DR EMBL; AP003286; BAD82016.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF06845.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS75431.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS75432.1; -; Genomic_DNA.
DR EMBL; AK067564; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK072312; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK103428; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015613349.1; XM_015757863.1. [Q5N941-1]
DR AlphaFoldDB; Q5N941; -.
DR SMR; Q5N941; -.
DR STRING; 4530.OS01T0870100-01; -.
DR PaxDb; Q5N941; -.
DR PRIDE; Q5N941; -.
DR EnsemblPlants; Os01t0870100-01; Os01t0870100-01; Os01g0870100. [Q5N941-1]
DR EnsemblPlants; Os01t0870100-02; Os01t0870100-02; Os01g0870100. [Q5N941-2]
DR GeneID; 4324936; -.
DR Gramene; Os01t0870100-01; Os01t0870100-01; Os01g0870100. [Q5N941-1]
DR Gramene; Os01t0870100-02; Os01t0870100-02; Os01g0870100. [Q5N941-2]
DR KEGG; osa:4324936; -.
DR eggNOG; ENOG502QU6W; Eukaryota.
DR InParanoid; Q5N941; -.
DR OMA; NHADTEG; -.
DR OrthoDB; 181406at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR ExpressionAtlas; Q5N941; baseline and differential.
DR Genevisible; Q5N941; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR044849; CASTOR/POLLUX/SYM8-like.
DR InterPro; IPR010420; CASTOR/POLLUX/SYM8_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR31563; PTHR31563; 1.
DR Pfam; PF06241; Castor_Poll_mid; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Ion channel; Ion transport; Membrane; Nucleus;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..965
FT /note="Probable ion channel POLLUX"
FT /id="PRO_0000004684"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 369..389
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 108..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..63
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 115..154
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 942..943
FT /note="AG -> GD (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_013298"
FT VAR_SEQ 944..965
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_013299"
SQ SEQUENCE 965 AA; 106215 MW; 533AF7592B4ECC08 CRC64;
MAESDGGEAS PSGGGGGEGS PDPRRPPARP QLTKSRTISG SAASAFDRWG TSNSSSSILV
RRSSTAPLPP GAAPRGLLTV AVDEPSYAAP NGGAAMLDRD WCYPSFLGPH ASRPRPPRSQ
QQTPTTTAAA AADSRSPTPA APPQTASVSQ REEEKSLASV VKRPMLLDER RSLSPPPPQQ
RAPRFDLSPY LVLMLVVTVI SFSLAIWQWM KATVLQEKIR SCCSVSTVDC KTTTEAFKIN
GQHGSDFINS ADWNLASCSR MLVFAIPVFL VKYIDQLRRR NTDSIRLRST EEEVPLKKRI
AYKVDVFFSG HPYAKLLALL LATIILIASG GIALYVVSGS GFLEALWLSW TFVADSGNHA
DQVGLGPRIV SVSISSGGML VFATMLGLVS DAISEKVDSW RKGKSEVIEV NHILILGWSD
KLGSLLKQLA IANKSIGGGV VVVLAERDKE EMEMDIGKLE FDFMGTSVIC RSGSPLILAD
LKKVSVSKAR AIIVLASDEN ADQSDARALR VVLSLTGVKE GLRGHVVVEM SDLDNEPLVK
LVGGELIETV VAHDVIGRLM IQCALQPGLA QIWEDILGFE NAEFYIKRWP ELDGMRFGDV
LISFPDAVPC GVKIASKAGK ILMNPDNDYV LQEGDEVLVI AEDDDTYVPA SLPQVRKGFL
PNIPTPPKYP EKILFCGWRR DIHDMIMVLE AFLAPGSELW MFNEVPEKER ERKLTDGGMD
IYGLTNIKLV HKEGNAVIRR HLESLPLETF DSILILADES VEDSIVHSDS RSLATLLLIR
DIQSKRLPSK ELKSPLRYNG FCHSSWIREM QHASDKSIII SEILDSRTRN LVSVSKISDY
VLSNELVSMA LAMVAEDKQI NRVLEELFAE EGNEMCIRSA EFYLYEQEEL SFFDIMVRAR
ERDEVVIGYR LANDDQAIIN PEQKSEIRKW SLDDVFVVIS KAGNATYFVK TTVMRSNPVV
YSSTF
