ID   POLR_NORAV              Reviewed;        2104 AA.
AC   Q27YG9;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 2.
DT   23-FEB-2022, entry version 64.
DE   RecName: Full=Replication polyprotein;
DE   Includes:
DE     RecName: Full=Membrane protein;
DE   Includes:
DE     RecName: Full=Helicase;
DE              EC=3.6.4.-;
DE   Includes:
DE     RecName: Full=Protease;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
GN   ORFNames=ORF2;
OS   Nora virus.
OC   Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses;
OC   unclassified ssRNA positive-strand viruses.
OX   NCBI_TaxID=363716;
OH   NCBI_TaxID=7227; Drosophila melanogaster (Fruit fly).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=16963764; DOI=10.1099/vir.0.81997-0;
RA   Habayeb M.S., Ekengren S.K., Hultmark D.;
RT   "Nora virus, a persistent virus in Drosophila, defines a new picorna-like
RT   virus family.";
RL   J. Gen. Virol. 87:3045-3051(2006).
RN   [2]
RP   SEQUENCE REVISION TO 1708-1721.
RA   Ekstrom J.-O., Habayeb M., Hultmark D.;
RL   Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The peptidase activity is involved in polyprotein maturation,
CC       possibly along with hosts proteases. Transmembrane protein may be
CC       surface viral glycoprotein. RNA-directed RNA polymerase replicates the
CC       viral genome (Probable). {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- PTM: Specific enzymatic cleavages in vivo yield mature proteins.
CC       {ECO:0000305}.
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DR   EMBL; DQ321720; ABC55268.2; -; Genomic_RNA.
DR   SMR; Q27YG9; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir.
DR   InterPro; IPR014759; Helicase_SF3_ssRNA_vir.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR001205; RNA-dir_pol_C.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   Pfam; PF00680; RdRP_1; 1.
DR   Pfam; PF00910; RNA_helicase; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
DR   PROSITE; PS51218; SF3_HELICASE_2; 1.
PE   4: Predicted;
KW   ATP-binding; Coiled coil; Helicase; Hydrolase; Membrane;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA-directed RNA polymerase;
KW   Transferase; Transmembrane; Transmembrane helix; Viral RNA replication.
FT   CHAIN           1..2104
FT                   /note="Replication polyprotein"
FT                   /id="PRO_0000283695"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          329..501
FT                   /note="SF3 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
FT   DOMAIN          1838..1965
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          1499..1520
FT                   /note="Viral peptidase"
FT                   /evidence="ECO:0000255"
FT   COILED          289..313
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   2104 AA;  239183 MW;  8C286C774AD588C3 CRC64;
     MLIEAFINSL LQNLGIMMSF RDLVASPWIL LVIAIPLCAF ASSASMVREM LFRHKITENI
     LKGTGVEELF NPFGIIIKYF LYFAILYAFI KYIRNNINVI TEKVNFIRRV VSNPTGTTGR
     RGVLGRCVEQ IVEYPTFFIT MVYELQQIKN KKDLISKITM ISSILKLPLG IWESTVGRML
     DRPAIEGTEE MLEDVLPIVA MGLTITKTQI GDVPVESFLV NLDRNQKACE NIIKRMQPLM
     IKMGMMKDSS YDTILQVAKE VNELSEAETW MKTTLKLNPN EFLQTQGAVR VGEIREKVAT
     LRNKLNTLQT KELRSDKVVT ECQKHLASLE VLLIEVKVLE NSNQTRVKPV GVTIQGEKQI
     GKTNLVAILS RKICEYVQEH GDISFRNATK WTTWSRQCRD EFDTGYTGQE ITYVDDAFQQ
     KDNKDHLMWF TFISNTAVGT NQADLKQKGL PYRSKLVFTT CNKSPDKSVT IEDIEALHAR
     FPHTICLRRN KNKMPKRGAI DESYDWVDFY YGPMSKAVSA IGSNTTSTLK TMSLSEIVKI
     IGDDLIIQNN FYNSTIKDVG ITGQEQMDGA QLERRQRMRE LRDHLLRIRS GDENMPFLDE
     TFELNSRPIQ TDEKFIPLKD NLDEEVMYGG ISDQLLTRFD NIIERSLEGY DVESRELGVE
     PLTTLNHVRS NMLSYRAWNL INSMCINKTE TFSAWLGRYI TECVEGVAEN LVTTKVKIRI
     NPFTGLQLIA AKRMLQENKL IDMDEIPSTS ANSYETVYDQ IKNFVNDELS LMETDVVDLA
     LAKISLSQIR SNIKRSTWLD VNDWILALKH KISGKSFAKH MDLYPSSLDS FLLTLKDWEV
     EDRIKFNSIY KQKVLFVQSR FSLYCWSPFI SRGTRFVKVT SRFRELVDKL ETGILFHEIK
     SVTNGIRWLG GAGNNGHVGE RVRVIAHTAQ FPKKSYPQNG FPINEELHRE WIQLVINSDY
     KYHSLIGEEK VNILWNLIRL QPQREVENFK VYLEDLQASP PKTGTICAKV VNDIKAEVTS
     SYRQFNNYYT RLTKDGMHTL LSMLSRIGVP ISDYWNDLLV DKAPAITAVT VGAITSLAII
     TIVKTFQYAI AGEEQSKGEK RAKQKNIATT KLQKLKFTLG KEQAEGDSIE HVKEFDGDVK
     FETIEKLFDH IDEHPNLNIV GLNLVAPENP IAIYAAREES YDFSFSEPRP PQWKKVVTFK
     EDSKRIISLQ LRGEDTEDNI LDEIEHAIKV SHGMPYAEWI FNGWFKKESN DNILYCVELD
     LVTAKTQSGP VGWTRAQTKN LKDLEIQLNK GKPIDVKSVV LGAPQASTQA TDTMDVLVNK
     HLVKVHCLSY ENLNNLALNG TQVFALASDN ILIVPAHAAR QNKWIRFSRA TQTGHYGVAK
     VDERRIDFTR DIAIAIILTR AEAEQKLCEL GYSIQLTNIS KEKFHFPLIT KYLLTADQSE
     VEWMNCTTLH YFAKNKTVGL GRTTSFQVSE FLCGNEYISK KLVACAQGLQ SSVELSRLGD
     CGSPIVLASG KKAGKLIGFH GYHSPNLQTW YGAMLTVEDL GIINGVEEHF DDPWAKLITQ
     GLPVDLPIGP EVEYVGNLIR PSLPVTNDSL DHWHKSPFAD QFEEQLAPGR LNPYDSYIEG
     DLPTNLEGRK SLILGPNSEM AKTLPELDQG ILDWIVDQLV VEQVATFKAE NLLTKVSDDI
     DEMLDYALNG NVDNTYVRGM EVNKASGLPW SLSGSPKKSD FIDVDEATGV RSFKVNANGD
     ALKNRVILKL QQAKMGNRIL SFSSSKLKDQ PIKIAQAKSG RTRVFHCIPV DLILFSGALY
     GPYKEAYTKA GLKCYHAVGI DPKSVGWQQL ATYMTKHPNY FDADYKNYDK YLHRQVFKAV
     RKIQRSVIQQ VCPDKWDKAR AVEELDAIDT YVVDYQTVYK TNRGNKSGSY TTTIDNCLAN
     DIYGLYAWVK TTGLRSLWDY RQNVSSVAFG DDIIKSVSDE YKDKYNYCTY RDVLNATGHI
     MTPGSKDGEE KPFTSFENLQ FLKRGFKLEN GMVLAPLLQR SIEGPFVWTD IREDQITVWV
     NLVQEQLIEA ALWGEEYYNE LCQKLKCGTN RTLNGALAVL LNTSWEVTFQ KFCNRYYGIK
     RGDL