ID   POLR_OYMV               Reviewed;        1776 AA.
AC   P20127;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=RNA replicase polyprotein;
DE            EC=2.7.7.48;
OS   Ononis yellow mosaic virus.
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Tymoviridae; Tymovirus.
OX   NCBI_TaxID=12153;
OH   NCBI_TaxID=58889; Ononis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2800337; DOI=10.1016/0042-6822(89)90198-0;
RA   Ding S.W., Keese P., Gibbs A.;
RT   "Nucleotide sequence of the ononis yellow mosaic tymovirus genome.";
RL   Virology 172:555-563(1989).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SIMILARITY: Belongs to the tymovirus NS35 RNA replicase polyprotein
CC       family. {ECO:0000305}.
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DR   EMBL; J04375; AAA46796.1; -; Genomic_RNA.
DR   PIR; JQ0106; RRWPYM.
DR   RefSeq; NP_041257.1; NC_001513.1.
DR   MEROPS; C21.001; -.
DR   GeneID; 1493968; -.
DR   KEGG; vg:1493968; -.
DR   Proteomes; UP000007789; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.90.70.100; -; 1.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR002588; Alphavirus-like_MT_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR008043; Peptidase_C21.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   InterPro; IPR043181; TYMV_endopept_dom.
DR   Pfam; PF05381; Peptidase_C21; 1.
DR   Pfam; PF00978; RdRP_2; 1.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   Pfam; PF01660; Vmethyltransf; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR   PROSITE; PS51738; PEPTIDASE_C21; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Hydrolase; Nucleotide-binding; Nucleotidyltransferase;
KW   Protease; RNA-directed RNA polymerase; Thiol protease; Transferase;
KW   Viral RNA replication.
FT   CHAIN           1..1776
FT                   /note="RNA replicase polyprotein"
FT                   /id="PRO_0000222936"
FT   DOMAIN          58..219
FT                   /note="Alphavirus-like MT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT   DOMAIN          658..812
FT                   /note="Peptidase C21"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01074"
FT   DOMAIN          866..1026
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          1027..1159
FT                   /note="(+)RNA virus helicase C-terminal"
FT   DOMAIN          1497..1603
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          546..583
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          595..615
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        546..582
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        711
FT                   /note="For protease activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01074"
FT   ACT_SITE        797
FT                   /note="For protease activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01074"
FT   BINDING         899..906
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   1776 AA;  198129 MW;  1C2E37B9EC3A5333 CRC64;
     MAFELALNAL ASSTHKDSSL NPVLNSAVQP LQTSLQNFPW IIGKEHLPFL LAAGIPTSGF
     GCNPHPHAVH KVIETFLLFN HWSFMATVQA SVMFMKPSKF KKLASVNPNF SELVNYRLTA
     ADSVRYPSTS TSLPKYEIVF MHDALMYFNP SQILDLFIQC PSLQRLHCSL VVPPESSFTD
     LSLHPNLYTY TISGNTLHYV PEGHHAGSYD QPLDAISWLK LNSIHSPHLN LSVSKLESWG
     PVHSLLITRG LPHLPSSEKQ QVSFHIPNCL PLPEATFLHQ PLRHRLVPTE VYDALFTYTR
     AVRTLRTSDP AGFVRTHSNK PQYSWVTSRA WDNLQTYALL NAPVRPVVLF DFFLSPLKKF
     QLFMSQHINS LVIKALPFLG LIPPLVKLTT LGFPIPSVSH FQILFFTMIG PSGQFILEAF
     PSMLHPAISY LESCGLVPRL PPPVAQQLQT MGILRKRSAF SLKTSLSTVK WPSWKITALI
     SALPAALSVF LKTISPLSLQ SLHDGYNAHL HPSPFNLSWS LETFHVQSPS PFLPLSLTPP
     SPSEEIALPP PIFRVPPPLP AQETPSPPAP ALVPPTQPPQ PQPWEEIIST FLSSLNASSH
     KPSPSSAPLE SPSPIPESFE VLAEAPQHQR SINECGALNQ LPLPVPSPLQ NSTNPKPPFP
     SSDLLSDMSC TGPVVEFETI FPAEYHMSNG SFPTRLRGHP RSSAPFPQKH CLLTAVASQL
     SYTEHQLWEF LCDMLPDSLL TNSEVENFGL STDHLTCLSY RLHFECIIHT SHSTIPYGIK
     KASTVIQISY IDGPPKHFKA FIKLAAAAPG SNPSKSNLVR AALRFKYNDA FLPFWDAHQH
     TISVPHAKNL ISNMKNGFDG ITSQLSGPSN KSPKMKLLEL DATIDVSFPR KCDVIHIAGF
     PGCGKSHPIQ KLLQTPAFRH FRLSVPTNEL RSEWKRDLNL PESEVWRLCT WETALFKSSN
     ILVVDEIYKL PRGYLDLILL ADPSIQLVIM LGDPLQGEYH SSHPSSSNSR LESETTRLSK
     YIDCYCWWTY RCPKAVADLF GVKTFNSNEG FIRAVLSHPP NLPNLVNSIA TANTMQSLGH
     HALTISSSQG MTYSDPVTVL LDRHSLLITP QTALVALTRS RSGIYFIGSM YTASGSAGTS
     YMFSCALTGL PVDMMSAFPL FHTLPLIHEP IRSRRHRLVA GHTPSLHVPP SNKWPHRLHL
     PPHIPTSHSK DVILAHGIVA SNAPERRLTT LHLPPTRLPL HFDLESCNPS TVSTSSTSNS
     EVPFTHAFLG ESFEELAAHF LPAHDPDLKE VTVGDQTSQQ FPYLDQPYTL SCQPSSLLAA
     SHKPASDPTL LIFSISKRLR FRASSSPYAF TPNDLILGHL LYTNWCKAFG RCPNSTIPFN
     PALFAECICL NEYAQLSSKT QATIVSNASR SDPDWRYTVV RIFAKSQHKV NDGSIFGSWK
     ACQTLALMHD FVILTLGPVK KYQRIIDHYD RPNFIYTHCG KTPSELSAWS HSFLKGDAYI
     CNDYTSFDQS QHGEAVIFES LKMHRVGIPR HLIDLHIYLK TNVSTQFGPL TCMRLTGEPG
     TYDDNTDYNL AVIFSQYVIS DHPIMVSGDD SVICGHPPIN PNWPAVEKLL HLRFKTEETS
     LPLFCGYYVG PTGCCRNPFA LFAKLMISYD KGNLFETLPS YLYEFSIGHR LGDVVRLLFP
     DHLLKYYSAC WDLFCRKCTA SQKLILSFEP IPPSFFSKLA STSRWVSKVL FSDLPTKIRD
     MLISSSKLPS YHQDPRVQYL ESELLTSFNH GRLSTN