POLX_TOBAC
ID POLX_TOBAC Reviewed; 1328 AA.
AC P10978;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Retrovirus-related Pol polyprotein from transposon TNT 1-94;
DE Includes:
DE RecName: Full=Protease;
DE EC=3.4.23.-;
DE Includes:
DE RecName: Full=Reverse transcriptase;
DE EC=2.7.7.49;
DE Includes:
DE RecName: Full=Endonuclease;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2536143; DOI=10.1038/337376a0;
RA Grandbastien M.-A., Spielmann A., Caboche M.;
RT "Tnt1, a mobile retroviral-like transposable element of tobacco isolated by
RT plant cell genetics.";
RL Nature 337:376-380(1989).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.49;
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DR EMBL; X13777; CAA32025.1; -; Genomic_DNA.
DR PIR; S04273; S04273.
DR AlphaFoldDB; P10978; -.
DR MEROPS; A11.002; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003964; F:RNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR025724; GAG-pre-integrase_dom.
DR InterPro; IPR001584; Integrase_cat-core.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR013103; RVT_2.
DR InterPro; IPR001878; Znf_CCHC.
DR InterPro; IPR036875; Znf_CCHC_sf.
DR Pfam; PF13976; gag_pre-integrs; 1.
DR Pfam; PF00665; rve; 1.
DR Pfam; PF07727; RVT_2; 1.
DR Pfam; PF00098; zf-CCHC; 1.
DR SMART; SM00343; ZnF_C2HC; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR SUPFAM; SSF57756; SSF57756; 1.
DR PROSITE; PS50994; INTEGRASE; 1.
DR PROSITE; PS50158; ZF_CCHC; 1.
PE 2: Evidence at transcript level;
KW Aspartyl protease; Endonuclease; Hydrolase; Metal-binding; Nuclease;
KW Nucleotidyltransferase; Protease; Reference proteome;
KW RNA-directed DNA polymerase; Transferase; Transposable element; Zinc;
KW Zinc-finger.
FT CHAIN 1..1328
FT /note="Retrovirus-related Pol polyprotein from transposon
FT TNT 1-94"
FT /id="PRO_0000199561"
FT DOMAIN 473..642
FT /note="Integrase catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
FT ZN_FING 230..247
FT /note="CCHC-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT REGION 189..265
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 729..800
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 197..218
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 243..257
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 729..750
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 767..800
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 297
FT /note="For protease activity"
FT /evidence="ECO:0000250"
SQ SEQUENCE 1328 AA; 151077 MW; F27E76C504B19B1B CRC64;
MSGVKYEVAK FNGDNGFSTW QRRMRDLLIQ QGLHKVLDVD SKKPDTMKAE DWADLDERAA
SAIRLHLSDD VVNNIIDEDT ARGIWTRLES LYMSKTLTNK LYLKKQLYAL HMSEGTNFLS
HLNVFNGLIT QLANLGVKIE EEDKAILLLN SLPSSYDNLA TTILHGKTTI ELKDVTSALL
LNEKMRKKPE NQGQALITEG RGRSYQRSSN NYGRSGARGK SKNRSKSRVR NCYNCNQPGH
FKRDCPNPRK GKGETSGQKN DDNTAAMVQN NDNVVLFINE EEECMHLSGP ESEWVVDTAA
SHHATPVRDL FCRYVAGDFG TVKMGNTSYS KIAGIGDICI KTNVGCTLVL KDVRHVPDLR
MNLISGIALD RDGYESYFAN QKWRLTKGSL VIAKGVARGT LYRTNAEICQ GELNAAQDEI
SVDLWHKRMG HMSEKGLQIL AKKSLISYAK GTTVKPCDYC LFGKQHRVSF QTSSERKLNI
LDLVYSDVCG PMEIESMGGN KYFVTFIDDA SRKLWVYILK TKDQVFQVFQ KFHALVERET
GRKLKRLRSD NGGEYTSREF EEYCSSHGIR HEKTVPGTPQ HNGVAERMNR TIVEKVRSML
RMAKLPKSFW GEAVQTACYL INRSPSVPLA FEIPERVWTN KEVSYSHLKV FGCRAFAHVP
KEQRTKLDDK SIPCIFIGYG DEEFGYRLWD PVKKKVIRSR DVVFRESEVR TAADMSEKVK
NGIIPNFVTI PSTSNNPTSA ESTTDEVSEQ GEQPGEVIEQ GEQLDEGVEE VEHPTQGEEQ
HQPLRRSERP RVESRRYPST EYVLISDDRE PESLKEVLSH PEKNQLMKAM QEEMESLQKN
GTYKLVELPK GKRPLKCKWV FKLKKDGDCK LVRYKARLVV KGFEQKKGID FDEIFSPVVK
MTSIRTILSL AASLDLEVEQ LDVKTAFLHG DLEEEIYMEQ PEGFEVAGKK HMVCKLNKSL
YGLKQAPRQW YMKFDSFMKS QTYLKTYSDP CVYFKRFSEN NFIILLLYVD DMLIVGKDKG
LIAKLKGDLS KSFDMKDLGP AQQILGMKIV RERTSRKLWL SQEKYIERVL ERFNMKNAKP
VSTPLAGHLK LSKKMCPTTV EEKGNMAKVP YSSAVGSLMY AMVCTRPDIA HAVGVVSRFL
ENPGKEHWEA VKWILRYLRG TTGDCLCFGG SDPILKGYTD ADMAGDIDNR KSSTGYLFTF
SGGAISWQSK LQKCVALSTT EAEYIAATET GKEMIWLKRF LQELGLHQKE YVVYCDSQSA
IDLSKNSMYH ARTKHIDVRY HWIREMVDDE SLKVLKISTN ENPADMLTKV VPRNKFELCK
ELVGMHSN
