POLY_DROME
ID POLY_DROME Reviewed; 1035 AA.
AC P10401; P10402;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Retrovirus-related Pol polyprotein from transposon gypsy;
DE Includes:
DE RecName: Full=Reverse transcriptase;
DE EC=2.7.7.49;
DE Includes:
DE RecName: Full=Endonuclease;
GN Name=pol;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3023871; DOI=10.1128/mcb.6.4.1129-1134.1986;
RA Marlor R.L., Parkhurst S.M., Corces V.G.;
RT "The Drosophila melanogaster gypsy transposable element encodes putative
RT gene products homologous to retroviral proteins.";
RL Mol. Cell. Biol. 6:1129-1134(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1318501; DOI=10.1007/bf00587562;
RA Smith P.A., Corces V.G.;
RT "The suppressor of Hairy-wing binding region is required for gypsy
RT mutagenesis.";
RL Mol. Gen. Genet. 233:65-70(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-950.
RX PubMed=2421255; DOI=10.1093/nar/14.7.3017;
RA Yuki S., Ishimaru S., Inouye S., Saigo K.;
RT "Identification of genes for reverse transcriptase-like enzymes in two
RT Drosophila retrotransposons, 412 and gypsy; a rapid detection method of
RT reverse transcriptase genes using YXDD box probes.";
RL Nucleic Acids Res. 14:3017-3030(1986).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU00405};
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA70219.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M12927; AAA70219.1; ALT_INIT; Genomic_DNA.
DR EMBL; X03734; CAA27371.1; -; Genomic_DNA.
DR PIR; B25666; GNFFG1.
DR AlphaFoldDB; P10401; -.
DR SMR; P10401; -.
DR MEROPS; A02.021; -.
DR PRIDE; P10401; -.
DR FlyBase; FBgn0014966; gypsy\pol.
DR PRO; PR:P10401; -.
DR GO; GO:0042575; C:DNA polymerase complex; IEA:UniProt.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProt.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003964; F:RNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR Gene3D; 2.40.70.10; -; 1.
DR Gene3D; 3.30.420.10; -; 2.
DR Gene3D; 3.30.70.270; -; 2.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR001584; Integrase_cat-core.
DR InterPro; IPR041588; Integrase_H2C2.
DR InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR000477; RT_dom.
DR InterPro; IPR041373; RT_RNaseH.
DR Pfam; PF17921; Integrase_H2C2; 1.
DR Pfam; PF17917; RT_RNaseH; 1.
DR Pfam; PF00078; RVT_1; 1.
DR SUPFAM; SSF50630; SSF50630; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS50994; INTEGRASE; 1.
DR PROSITE; PS50878; RT_POL; 1.
PE 4: Predicted;
KW Endonuclease; Hydrolase; Nuclease; Nucleotidyltransferase;
KW RNA-directed DNA polymerase; Transferase; Transposable element.
FT CHAIN 1..1035
FT /note="Retrovirus-related Pol polyprotein from transposon
FT gypsy"
FT /id="PRO_0000058512"
FT DOMAIN 205..390
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT DOMAIN 797..956
FT /note="Integrase catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
FT CONFLICT 31..32
FT /note="DA -> AR (in Ref. 3; CAA27371)"
FT /evidence="ECO:0000305"
FT CONFLICT 60
FT /note="G -> R (in Ref. 3; CAA27371)"
FT /evidence="ECO:0000305"
FT CONFLICT 232
FT /note="F -> S (in Ref. 3; CAA27371)"
FT /evidence="ECO:0000305"
FT CONFLICT 490
FT /note="D -> V (in Ref. 3; CAA27371)"
FT /evidence="ECO:0000305"
FT CONFLICT 883
FT /note="S -> T (in Ref. 3; CAA27371)"
FT /evidence="ECO:0000305"
FT CONFLICT 923
FT /note="N -> S (in Ref. 3; CAA27371)"
FT /evidence="ECO:0000305"
FT CONFLICT 947..950
FT /note="RPIE -> NQLR (in Ref. 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1035 AA; 117818 MW; 7B985CA272B8A5A6 CRC64;
RLVEFFRGRS RLPFIERRLA GRTLKMLIDT DAAKNYIRPV KELKNVMPVA SPFSVSSIHG
STEIKHKCLM KVFKHISPFF LLDSLNAFDA IIGLDLLTQA GVKLNLAEDS LEYQGIAEKL
HYFSCPSVNF TDVNDIVVPD SVKKEFKDTI IRRKKAFSTT NEALPFNTAV TATIRTVDNE
PVYSRAYPTL MGVSDFVNNE VKQLLKDGII RPSRSPYNSP TWVVDKKGTD AFGNPNKRLV
IDFRKLNEKT IPDRYPMPSI PMILANLGKA KFFTTLDLKS GYHQIYLAEH DREKTSFSVN
GGKYEFCRLP FGLRNASSIF QRALDDVLRE QIGKICYVYV DDVIIFSENE SDHVRHIDTV
LKCLIDANMR VSQEKTRFFK ESVEYLGFIV SKDGTKSDPE KVKAIQEYPE PDCVYKVRSF
LGLASYYRVF IKDFAAIARP ITDILKGENG SVSKHMSKKI PVEFNETQRN AFQRLRNILA
SEDVILKYPD FKKPFDLTTD ASASGIGAVL SQEGRPITMI SRTLKQPEQN YATNERELLA
IVWALGKLQN FLYGSREINI FTDHQPLTFA VADRNTNAKI KRWKSYIDQH NAKVFYKPGK
ENFVADALSR QNLNALQNEP QSDAATIHSE LSLTYTVETT DKPLNCFRNQ IILEAARFPL
KRNLVLFRSK SRHLISFTDK SWLLKTLKEV VNPDVVNAIH CDLPTLASFQ HDLIAHFPAT
QFRHCKNVVL DITDKNEQIE IVTAEHNRAH RAAQENIKQV LRDYYFPKMG SLAKEVVANC
RVCTQAKYDR HPKKQELGET PIPSYTGEMV HIDIFSTDRK LFLTCIDKFS KYAIVQPVVS
RTIVDITAPL LQIINLFPNI KTVYCDNEPA FNSETVTSML KNSFGIDIVN APPLHSSSNG
QVERFHSTLA EIARCLKLDK KTNDTVELIL RATIEYNKTV HSVTRERPIE VVHPGAHERC
LEIKARLVKA QQDSIGRNNP SRQNRVFEVG ERVFVKNNKR LGNKLTPLCT EQKVQADLGT
SVLIKGRVVH KDNLK
