ID   POL_MCFF3               Reviewed;         156 AA.
AC   P16103;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   23-FEB-2022, entry version 54.
DE   RecName: Full=Gag-Pol polyprotein;
DE            Short=Pr180gag-pol;
DE   Contains:
DE     RecName: Full=Integrase p46;
DE              Short=IN;
DE              EC=2.7.7.- {ECO:0000250|UniProtKB:P03355};
DE              EC=3.1.-.- {ECO:0000250|UniProtKB:P03355};
DE   Flags: Fragment;
GN   Name=gag-pol;
OS   Mink cell focus-forming murine leukemia virus (isolate CI-3).
OC   Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC   Ortervirales; Retroviridae; Orthoretrovirinae; Gammaretrovirus;
OC   Murine leukemia virus.
OX   NCBI_TaxID=11936;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=6319752; DOI=10.1128/jvi.49.2.530-539.1984;
RA   Mark G.E., Rapp U.R.;
RT   "Envelope gene sequence of two in vitro-generated mink cell focus-forming
RT   murine leukemia viruses which contain the entire gp70 sequence of the
RT   endogenous nonecotropic parent.";
RL   J. Virol. 49:530-539(1984).
CC   -!- FUNCTION: Integrase catalyzes viral DNA integration into the host
CC       chromosome, by performing a series of DNA cutting and joining
CC       reactions. This enzyme activity takes place after virion entry into a
CC       cell and reverse transcription of the RNA genome in dsDNA. The first
CC       step in the integration process is 3' processing. This step requires a
CC       complex comprising the viral genome, matrix protein and integrase. This
CC       complex is called the pre-integration complex (PIC). The integrase
CC       protein removes 2 nucleotides from each 3' end of the viral DNA,
CC       leaving recessed CA OH's at the 3' ends. In the second step that
CC       requires cell division, the PIC enters cell nucleus. In the third step,
CC       termed strand transfer, the integrase protein joins the previously
CC       processed 3' ends to the 5' ends of strands of target cellular DNA at
CC       the site of integration. The last step is viral DNA integration into
CC       host chromosome (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Magnesium ions are required for integrase activity. Binds at least
CC       1, maybe 2 magnesium ions. {ECO:0000250};
CC   -!- PTM: Specific enzymatic cleavages by the viral protease yield mature
CC       proteins. The protease is released by autocatalytic cleavage. The
CC       polyprotein is cleaved during and after budding, this process is termed
CC       maturation (By similarity). {ECO:0000250}.
CC   -!- MISCELLANEOUS: This protein is translated as a gag-pol fusion protein
CC       by episodic readthrough of the gag protein termination codon.
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DR   EMBL; K02725; AAA46374.1; -; Genomic_RNA.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   InterPro; IPR040643; MLVIN_C.
DR   Pfam; PF18697; MLVIN_C; 1.
PE   3: Inferred from homology;
KW   DNA integration; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease; Nucleotidyltransferase; Transferase; Viral genome integration;
KW   Virus entry into host cell.
FT   CHAIN           <1..156
FT                   /note="Gag-Pol polyprotein"
FT                   /id="PRO_0000125489"
FT   CHAIN           <1..156
FT                   /note="Integrase p46"
FT                   /id="PRO_0000390821"
FT   NON_TER         1
SQ   SEQUENCE   156 AA;  17366 MW;  13FF17128256F73D CRC64;
     RNTPGPHGLT PYEILYGAPP PLVNFHDPDM SELTNSPSLQ AHLQALQTVQ REIWRPLAEA
     YRDRLDQPVI PHPFRTGDSV WVRRHQTKNS EPRWKGPYTV LLTTPTALKV DGIAAWIHAA
     HVKAATTPPA GTASGPTWKV QRSQNPLKIR LTRGAP