ID   POL_MLVRK               Reviewed;         581 AA.
AC   P31795;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   29-SEP-2021, entry version 91.
DE   RecName: Full=Pol polyprotein;
DE   Contains:
DE     RecName: Full=Reverse transcriptase/ribonuclease H;
DE              Short=RT;
DE              EC=2.7.7.49;
DE              EC=3.1.26.4;
DE   Contains:
DE     RecName: Full=Integrase;
DE              Short=IN;
DE              EC=2.7.7.- {ECO:0000250|UniProtKB:P03355};
DE              EC=3.1.-.- {ECO:0000250|UniProtKB:P03355};
DE   Flags: Fragment;
GN   Name=pol;
OS   Radiation murine leukemia virus (strain Kaplan).
OC   Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC   Ortervirales; Retroviridae; Orthoretrovirinae; Gammaretrovirus;
OC   Murine leukemia virus.
OX   NCBI_TaxID=31689;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1629969; DOI=10.1128/jvi.66.8.5141-5146.1992;
RA   Poliquin L., Bergeron D., Fortier J.L., Paquette Y., Bergeron R.,
RA   Rassart E.;
RT   "Determinants of thymotropism in Kaplan radiation leukemia virus and
RT   nucleotide sequence of its envelope region.";
RL   J. Virol. 66:5141-5146(1992).
CC   -!- FUNCTION: During replicative cycle of retroviruses, the reverse-
CC       transcribed viral DNA is integrated into the host chromosome by the
CC       viral integrase enzyme. RNase H activity is associated with the reverse
CC       transcriptase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00408};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.49;
CC   -!- PTM: Specific enzymatic cleavages in vivo yield mature proteins.
CC   -!- MISCELLANEOUS: This protein is synthesized as a Gag-Pol polyprotein.
CC   -!- SIMILARITY: Belongs to the retroviral Pol polyprotein family.
CC       {ECO:0000305}.
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DR   EMBL; M93052; AAA46525.1; -; Genomic_DNA.
DR   PIR; A42743; A42743.
DR   SMR; P31795; -.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0003964; F:RNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004523; F:RNA-DNA hybrid ribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 2.
DR   InterPro; IPR039464; Gag-pol_Znf-H3C2.
DR   InterPro; IPR001584; Integrase_cat-core.
DR   InterPro; IPR040643; MLVIN_C.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR002156; RNaseH_domain.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF18697; MLVIN_C; 1.
DR   Pfam; PF00075; RNase_H; 1.
DR   Pfam; PF00665; rve; 1.
DR   Pfam; PF16721; zf-H3C2; 1.
DR   SUPFAM; SSF53098; SSF53098; 2.
DR   PROSITE; PS50994; INTEGRASE; 1.
DR   PROSITE; PS50879; RNASE_H_1; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; Endonuclease; Hydrolase;
KW   Multifunctional enzyme; Nuclease; Nucleotidyltransferase;
KW   RNA-directed DNA polymerase; Transferase; Viral genome integration;
KW   Virus entry into host cell.
FT   CHAIN           <1..581
FT                   /note="Pol polyprotein"
FT                   /id="PRO_0000199555"
FT   CHAIN           <1..?
FT                   /note="Reverse transcriptase/ribonuclease H"
FT                   /id="PRO_0000259737"
FT   CHAIN           ?..581
FT                   /note="Integrase"
FT                   /id="PRO_0000259738"
FT   DOMAIN          20..166
FT                   /note="RNase H type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00408"
FT   DOMAIN          290..448
FT                   /note="Integrase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00457"
FT   NON_TER         1
SQ   SEQUENCE   581 AA;  65158 MW;  8D7A38694C8E036E CRC64;
     LEILAETHGT RPDLTDQPIP DADHTWYTDG SSFLQEGQRK AGAAVTTETE VIWARALPAG
     TSAQRAELIA LTQALKMAEG KRLNVYTDSR YAFATAHIHG EIYKRRGLLT SEGREIKNKS
     EILALLKALF LPKRLSIIHC LGHQKGDSAE ARGNRLADQA AREAAIKAPP DTSTLLIEDS
     TPYTPAYFHY TETDLKKLRE LGATYNQSQG YWVFQGKPVM PDQFVFELLD SLHRLTHLGY
     QKMKALLDRG ESPYYMLNRD KTLQYVADSC TVCAQVNASK AKIGAGVRVR GHRPGTHWEI
     DFTEVKPGLY GYKYLLVFVD TFSGWVEAFP TKHETAKIVT KKLLEEIFPR FGMPQVLGTD
     NGPAFVSQVS QSVAKLLGID WKLHCAYRPQ SSGQVERMNR TIKETLTKLT LATGTRDWVL
     LLPLALYRAR NTPGPHGLTP YEILYGAPPP LVNFHDPEMS KFTNSPSLQA HLQALQAVQR
     EVWKPLAAAY QDQLDQPVIP HPFRVGDTVW VRRHQTKNLE PRWKGPYTVL LTTPTALKVD
     GISAWIHAAH VKAATTPPIR PSWRVQRSQN PLKIRLTRGA P