POM33_YEAST
ID POM33_YEAST Reviewed; 279 AA.
AC Q12164; D6VXY1;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Pore membrane protein of 33 kDa;
GN Name=POM33; OrderedLocusNames=YLL023C; ORFNames=L1201;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9046100;
RX DOI=10.1002/(sici)1097-0061(199702)13:2<183::aid-yea65>3.0.co;2-v;
RA Purnelle B., Goffeau A.;
RT "The sequence of 32kb on the left arm of yeast chromosome XII reveals six
RT known genes, a new member of the seripauperins family and a new ABC
RT transporter homologous to the human multidrug resistance protein.";
RL Yeast 13:183-188(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [7]
RP SUBCELLULAR LOCATION, INTERACTION WITH RTN1, AND FUNCTION.
RX PubMed=20498018; DOI=10.1083/jcb.200910043;
RA Chadrin A., Hess B., San Roman M., Gatti X., Lombard B., Loew D.,
RA Barral Y., Palancade B., Doye V.;
RT "Pom33, a novel transmembrane nucleoporin required for proper nuclear pore
RT complex distribution.";
RL J. Cell Biol. 189:795-811(2010).
CC -!- FUNCTION: Contributes to proper distribution and/or efficient assembly
CC of nuclear pores. Required for normal pore density in the daughter
CC nucleus during telophase. {ECO:0000269|PubMed:20498018}.
CC -!- SUBUNIT: Interacts with RTN1. {ECO:0000269|PubMed:20498018}.
CC -!- INTERACTION:
CC Q12164; P32793: YSC84; NbExp=2; IntAct=EBI-30000, EBI-24460;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC membrane protein. Nucleus, nuclear pore complex.
CC -!- MISCELLANEOUS: Present with 1680 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the PER33/POM33 family. {ECO:0000305}.
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DR EMBL; X97560; CAA66168.1; -; Genomic_DNA.
DR EMBL; Z73128; CAA97471.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09297.1; -; Genomic_DNA.
DR PIR; S64771; S64771.
DR RefSeq; NP_013077.1; NM_001181843.1.
DR AlphaFoldDB; Q12164; -.
DR BioGRID; 31230; 264.
DR DIP; DIP-4749N; -.
DR IntAct; Q12164; 5.
DR MINT; Q12164; -.
DR STRING; 4932.YLL023C; -.
DR iPTMnet; Q12164; -.
DR MaxQB; Q12164; -.
DR PaxDb; Q12164; -.
DR PRIDE; Q12164; -.
DR EnsemblFungi; YLL023C_mRNA; YLL023C; YLL023C.
DR GeneID; 850637; -.
DR KEGG; sce:YLL023C; -.
DR SGD; S000003946; POM33.
DR VEuPathDB; FungiDB:YLL023C; -.
DR eggNOG; KOG4002; Eukaryota.
DR GeneTree; ENSGT00390000011368; -.
DR HOGENOM; CLU_065417_2_0_1; -.
DR InParanoid; Q12164; -.
DR OMA; VSYAIVC; -.
DR BioCyc; YEAST:G3O-32127-MON; -.
DR PRO; PR:Q12164; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q12164; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031309; C:integral component of nuclear outer membrane; ISM:SGD.
DR GO; GO:0005643; C:nuclear pore; IEA:UniProtKB-SubCell.
DR GO; GO:0071786; P:endoplasmic reticulum tubular network organization; IBA:GO_Central.
DR GO; GO:0061024; P:membrane organization; IBA:GO_Central.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR005344; TMEM33/Pom33.
DR Pfam; PF03661; TMEM33_Pom33; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; mRNA transport; Nuclear pore complex;
KW Nucleus; Protein transport; Reference proteome; Translocation;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..279
FT /note="Pore membrane protein of 33 kDa"
FT /id="PRO_0000247116"
FT TOPO_DOM 1..31
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..56
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..124
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..180
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 202..203
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 222..279
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 279 AA; 32186 MW; C55F69F751B925E3 CRC64;
MSSRPANNQG PPNLPARDKS LVQRFMAVAK SLQFAWFTGH SVVLISSILY LLKMSEFYYR
SAYLGVIESF GIIIYQQFFT RNEPLQTQDA AATKASIKSR VAGLLKSEDV LYLVLANFWL
FTPRFSFSLI PFFAFAVFHV LIYVEKVLLP KVFHLSSKDS SKILSFIDKF VVQYNDLCMH
WVGTAELLIF ILVLFRAILC FQRSWIILVV YAIFIKLRYE NSKYMKAAFA QWRVRMDGII
SHPSIPPFVK RAYNAIKMSL IRLSEYRLSG APQVTKKQN
