PONC2_DICDI
ID PONC2_DICDI Reviewed; 147 AA.
AC Q54LC3;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Ponticulin-like protein C2;
DE Flags: Precursor;
GN Name=ponC2; ORFNames=DDB_G0286715;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP FUNCTION.
RX PubMed=18522444; DOI=10.1021/la800085n;
RA Barfoot R.J., Sheikh K.H., Johnson B.R., Colyer J., Miles R.E.,
RA Jeuken L.J., Bushby R.J., Evans S.D.;
RT "Minimal F-actin cytoskeletal system for planar supported phospholipid
RT bilayers.";
RL Langmuir 24:6827-6836(2008).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000305}.
CC -!- PTM: The GPI-like-anchor contains a phosphoceramide group, rather than
CC a phosphatidyl group. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ponticulin family. {ECO:0000305}.
CC -!- CAUTION: The Dictyosteliida are known to produce a
CC glycosylsphingolipidinositol anchor (GPI-like-anchor). It has not been
CC established whether Dictyosteliida make a glycosylphosphatidylinositol
CC anchor (GPI-anchor) also, and whether their GPI-like-anchor
CC modifications can be interconverted with GPI-anchor modifications in a
CC resculpting process. It has not been established that the GPI-like-
CC anchor modification in Dictyosteliida utilizes the same sequence motif.
CC {ECO:0000305}.
CC -!- CAUTION: Different sequence motifs predict both the N-glycosylation
CC modification and the GPI- or GPI-like anchor modification for Asn-118.
CC While it is chemically possible for both modifications to occur, it is
CC not known whether it is enzymatically possible. {ECO:0000305}.
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DR EMBL; AAFI02000089; EAL64113.1; -; Genomic_DNA.
DR RefSeq; XP_637638.1; XM_632546.1.
DR AlphaFoldDB; Q54LC3; -.
DR STRING; 44689.DDB0232286; -.
DR PaxDb; Q54LC3; -.
DR EnsemblProtists; EAL64113; EAL64113; DDB_G0286715.
DR GeneID; 8625779; -.
DR KEGG; ddi:DDB_G0286715; -.
DR dictyBase; DDB_G0286715; ponC2.
DR HOGENOM; CLU_1771535_0_0_1; -.
DR InParanoid; Q54LC3; -.
DR PhylomeDB; Q54LC3; -.
DR PRO; PR:Q54LC3; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; ISS:dictyBase.
DR GO; GO:0042599; C:lamellar body; HDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051015; F:actin filament binding; ISS:dictyBase.
PE 3: Inferred from homology;
KW Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..118
FT /note="Ponticulin-like protein C2"
FT /id="PRO_0000312136"
FT PROPEP 119..147
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000312137"
FT LIPID 118
FT /note="GPI-like-anchor amidated asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 118
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 147 AA; 15267 MW; 03F1316366AB7356 CRC64;
MKFTKPLLLL IVAIIASSNA AETFSNFQVT NSDASSPCVT TPVELKVNTC QSACGSILNV
LPVTGSTSKF TFNQFGAQDT KCAATPTSSN EFTCVDGKSK VAIGTTTYSV VCVPDKTNSS
ESDSSDSTRI GASFALFALA LLSMLAL
