ID   PONC4_DICDI             Reviewed;         147 AA.
AC   Q54LC1;
DT   04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Ponticulin-like protein C4;
DE   Flags: Precursor;
GN   Name=ponC4; ORFNames=DDB_G0286719;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   FUNCTION.
RX   PubMed=18522444; DOI=10.1021/la800085n;
RA   Barfoot R.J., Sheikh K.H., Johnson B.R., Colyer J., Miles R.E.,
RA   Jeuken L.J., Bushby R.J., Evans S.D.;
RT   "Minimal F-actin cytoskeletal system for planar supported phospholipid
RT   bilayers.";
RL   Langmuir 24:6827-6836(2008).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC       anchor {ECO:0000305}.
CC   -!- PTM: The GPI-like-anchor contains a phosphoceramide group, rather than
CC       a phosphatidyl group. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ponticulin family. {ECO:0000305}.
CC   -!- CAUTION: The Dictyosteliida are known to produce a
CC       glycosylsphingolipidinositol anchor (GPI-like-anchor). It has not been
CC       established whether Dictyosteliida make a glycosylphosphatidylinositol
CC       anchor (GPI-anchor) also, and whether their GPI-like-anchor
CC       modifications can be interconverted with GPI-anchor modifications in a
CC       resculpting process. It has not been established that the GPI-like-
CC       anchor modification in Dictyosteliida utilizes the same sequence motif.
CC       {ECO:0000305}.
CC   -!- CAUTION: Different sequence motifs predict both the N-glycosylation
CC       modification and the GPI- or GPI-like anchor modification for Asn-118.
CC       While it is chemically possible for both modifications to occur, it is
CC       not known whether it is enzymatically possible. {ECO:0000305}.
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DR   EMBL; AAFI02000089; EAL64115.1; -; Genomic_DNA.
DR   RefSeq; XP_637640.1; XM_632548.1.
DR   AlphaFoldDB; Q54LC1; -.
DR   STRING; 44689.DDB0232289; -.
DR   PaxDb; Q54LC1; -.
DR   EnsemblProtists; EAL64115; EAL64115; DDB_G0286719.
DR   GeneID; 8625781; -.
DR   KEGG; ddi:DDB_G0286719; -.
DR   dictyBase; DDB_G0286719; ponC4.
DR   HOGENOM; CLU_1771535_0_0_1; -.
DR   InParanoid; Q54LC1; -.
DR   PhylomeDB; Q54LC1; -.
DR   PRO; PR:Q54LC1; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016021; C:integral component of membrane; ISS:dictyBase.
DR   GO; GO:0042599; C:lamellar body; HDA:dictyBase.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051015; F:actin filament binding; ISS:dictyBase.
PE   3: Inferred from homology;
KW   Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW   Reference proteome; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..118
FT                   /note="Ponticulin-like protein C4"
FT                   /id="PRO_0000312140"
FT   PROPEP          119..147
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000312141"
FT   LIPID           118
FT                   /note="GPI-like-anchor amidated asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        118
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   147 AA;  15159 MW;  82183A506B8E302A CRC64;
     MKFTKSLLLL IVAVFASSNA AETFSNFQVT NSEASSPCVT TPVELKVNTC QSACGSILNV
     LPVTGSTSKF TFNQFGAQDT KCAATPTSSN EFTCVDGKSK VAIGSTTYSV VCVPDKTNSS
     ESDSSDSTRI GASFALAAAA LLSMIAL