ID   POND_DICDI              Reviewed;         148 AA.
AC   Q54SJ8;
DT   04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Ponticulin-like protein D;
DE   Flags: Precursor;
GN   Name=ponD; ORFNames=DDB_G0282423;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   FUNCTION.
RX   PubMed=18522444; DOI=10.1021/la800085n;
RA   Barfoot R.J., Sheikh K.H., Johnson B.R., Colyer J., Miles R.E.,
RA   Jeuken L.J., Bushby R.J., Evans S.D.;
RT   "Minimal F-actin cytoskeletal system for planar supported phospholipid
RT   bilayers.";
RL   Langmuir 24:6827-6836(2008).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC       anchor {ECO:0000305}.
CC   -!- PTM: The GPI-like-anchor contains a phosphoceramide group, rather than
CC       a phosphatidyl group. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ponticulin family. {ECO:0000305}.
CC   -!- CAUTION: The Dictyosteliida are known to produce a
CC       glycosylsphingolipidinositol anchor (GPI-like-anchor). It has not been
CC       established whether Dictyosteliida make a glycosylphosphatidylinositol
CC       anchor (GPI-anchor) also, and whether their GPI-like-anchor
CC       modifications can be interconverted with GPI-anchor modifications in a
CC       resculpting process. It has not been established that the GPI-like-
CC       anchor modification in Dictyosteliida utilizes the same sequence motif.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000047; EAL66071.1; -; Genomic_DNA.
DR   RefSeq; XP_640041.1; XM_634949.1.
DR   AlphaFoldDB; Q54SJ8; -.
DR   PaxDb; Q54SJ8; -.
DR   EnsemblProtists; EAL66071; EAL66071; DDB_G0282423.
DR   GeneID; 8623567; -.
DR   KEGG; ddi:DDB_G0282423; -.
DR   dictyBase; DDB_G0282423; ponD.
DR   HOGENOM; CLU_1762188_0_0_1; -.
DR   PRO; PR:Q54SJ8; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016021; C:integral component of membrane; ISS:dictyBase.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051015; F:actin filament binding; ISS:dictyBase.
PE   3: Inferred from homology;
KW   Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW   Reference proteome; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..125
FT                   /note="Ponticulin-like protein D"
FT                   /id="PRO_0000312144"
FT   PROPEP          126..148
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000312145"
FT   LIPID           125
FT                   /note="GPI-like-anchor amidated aspartate"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        67
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   148 AA;  15356 MW;  255273FCE5093226 CRC64;
     MLLNKSLLLL VAFVFAIVSA TTYSEFKITG TNPLTQETCD PSIVYTSQNG ACQGVCGMFG
     KLVATSNSTQ FNVEMYGSAG CVGPLGTTGL TCLPNEQVIK VTETISVVCF ADKDEPSGDD
     SSGDDSSAAA TMIASFSAIL IALLFALL