ID   PONE_DICDI              Reviewed;         171 AA.
AC   Q54BG3;
DT   04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Ponticulin-like protein E;
DE   Flags: Precursor;
GN   Name=ponE; ORFNames=DDB_G0293634;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   FUNCTION.
RX   PubMed=18522444; DOI=10.1021/la800085n;
RA   Barfoot R.J., Sheikh K.H., Johnson B.R., Colyer J., Miles R.E.,
RA   Jeuken L.J., Bushby R.J., Evans S.D.;
RT   "Minimal F-actin cytoskeletal system for planar supported phospholipid
RT   bilayers.";
RL   Langmuir 24:6827-6836(2008).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC       anchor {ECO:0000305}.
CC   -!- PTM: The GPI-like-anchor contains a phosphoceramide group, rather than
CC       a phosphatidyl group. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ponticulin family. {ECO:0000305}.
CC   -!- CAUTION: The Dictyosteliida are known to produce a
CC       glycosylsphingolipidinositol anchor (GPI-like-anchor). It has not been
CC       established whether Dictyosteliida make a glycosylphosphatidylinositol
CC       anchor (GPI-anchor) also, and whether their GPI-like-anchor
CC       modifications can be interconverted with GPI-anchor modifications in a
CC       resculpting process. It has not been established that the GPI-like-
CC       anchor modification in Dictyosteliida utilizes the same sequence motif.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000218; EAL60629.1; -; Genomic_DNA.
DR   RefSeq; XP_629066.1; XM_629064.1.
DR   AlphaFoldDB; Q54BG3; -.
DR   PaxDb; Q54BG3; -.
DR   EnsemblProtists; EAL60629; EAL60629; DDB_G0293634.
DR   GeneID; 8629358; -.
DR   KEGG; ddi:DDB_G0293634; -.
DR   dictyBase; DDB_G0293634; ponE.
DR   HOGENOM; CLU_1809794_0_0_1; -.
DR   PRO; PR:Q54BG3; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016021; C:integral component of membrane; ISS:dictyBase.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051015; F:actin filament binding; ISS:dictyBase.
PE   3: Inferred from homology;
KW   Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW   Reference proteome; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..144
FT                   /note="Ponticulin-like protein E"
FT                   /id="PRO_0000312146"
FT   PROPEP          145..171
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000312147"
FT   REGION          109..145
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           144
FT                   /note="GPI-like-anchor amidated serine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        39
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        95
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        120
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   171 AA;  18932 MW;  39C61D67D9993951 CRC64;
     MQTFKKLILI LLLLALFVFS NGQYTFNVLN KAPGSKCENG TMAELNTCSK DCLTSFLILK
     SIDKKSLTFT TFNNNQCNGD YNTQTTFDCK PTPQNISQTQ YFISCEEQTS KPTSSPIHSN
     STIKSTSTTT TSTPLPHKDT PHHSASSSSI VVRLTPIFFI AFASLIFLFG F