ID   PONH_DICDI              Reviewed;         203 AA.
AC   Q54LM2;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Ponticulin-like protein H;
DE   Flags: Precursor;
GN   Name=ponH; ORFNames=DDB_G0286573;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   FUNCTION.
RX   PubMed=18522444; DOI=10.1021/la800085n;
RA   Barfoot R.J., Sheikh K.H., Johnson B.R., Colyer J., Miles R.E.,
RA   Jeuken L.J., Bushby R.J., Evans S.D.;
RT   "Minimal F-actin cytoskeletal system for planar supported phospholipid
RT   bilayers.";
RL   Langmuir 24:6827-6836(2008).
CC   -!- FUNCTION: Binds F-actin and nucleates actin assembly.
CC       {ECO:0000269|PubMed:18522444}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC       anchor {ECO:0000305}.
CC   -!- PTM: The GPI-like-anchor contains a phosphoceramide group, rather than
CC       a phosphatidyl group. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ponticulin family. {ECO:0000305}.
CC   -!- CAUTION: The Dictyosteliida are known to produce a
CC       glycosylsphingolipidinositol anchor (GPI-like-anchor). It has not been
CC       established whether Dictyosteliida make a glycosylphosphatidylinositol
CC       anchor (GPI-anchor) also, and whether their GPI-like-anchor
CC       modifications can be interconverted with GPI-anchor modifications in a
CC       resculpting process. It has not been established that the GPI-like-
CC       anchor modification in Dictyosteliida utilizes the same sequence motif.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000089; EAL64042.1; -; Genomic_DNA.
DR   RefSeq; XP_637539.1; XM_632447.1.
DR   AlphaFoldDB; Q54LM2; -.
DR   STRING; 44689.DDB0237613; -.
DR   PaxDb; Q54LM2; -.
DR   EnsemblProtists; EAL64042; EAL64042; DDB_G0286573.
DR   GeneID; 8625678; -.
DR   KEGG; ddi:DDB_G0286573; -.
DR   dictyBase; DDB_G0286573; ponH.
DR   eggNOG; ENOG502RINX; Eukaryota.
DR   HOGENOM; CLU_1351064_0_0_1; -.
DR   PRO; PR:Q54LM2; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Actin-binding; Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein;
KW   Membrane; Reference proteome; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..182
FT                   /note="Ponticulin-like protein H"
FT                   /id="PRO_0000367833"
FT   PROPEP          183..203
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000367834"
FT   REGION          127..183
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        127..164
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           182
FT                   /note="GPI-like-anchor amidated serine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        58
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   203 AA;  20730 MW;  93B57F8E305EE97E CRC64;
     MKLLNSLVLL AALCAITANG KIVEDTPDPS TVYNLFQLSS SDGGCDPAGT HSPDANVNVS
     VDKCRNVCNK NIKISKGTST NQFTFQTYND NSCSQATSDQ ALSFTCSDNV KKQLGTSIYS
     VICSTGSDST NPTSTPSTTP SATPTVTPST TPTVTPTVTP STTPTVAPTV PPTTPPSTTT
     GSGSTVVASF GLIVSILLAS LAL