ID   PONL_DICDI              Reviewed;         144 AA.
AC   Q54IM7;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Ponticulin-like protein L;
DE   Flags: Precursor;
GN   Name=ponL; ORFNames=DDB_G0288649;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   FUNCTION.
RX   PubMed=18522444; DOI=10.1021/la800085n;
RA   Barfoot R.J., Sheikh K.H., Johnson B.R., Colyer J., Miles R.E.,
RA   Jeuken L.J., Bushby R.J., Evans S.D.;
RT   "Minimal F-actin cytoskeletal system for planar supported phospholipid
RT   bilayers.";
RL   Langmuir 24:6827-6836(2008).
CC   -!- FUNCTION: Binds F-actin and nucleates actin assembly.
CC       {ECO:0000269|PubMed:18522444}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC       anchor {ECO:0000305}.
CC   -!- PTM: The GPI-like-anchor contains a phosphoceramide group, rather than
CC       a phosphatidyl group. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ponticulin family. {ECO:0000305}.
CC   -!- CAUTION: The Dictyosteliida are known to produce a
CC       glycosylsphingolipidinositol anchor (GPI-like-anchor). It has not been
CC       established whether Dictyosteliida make a glycosylphosphatidylinositol
CC       anchor (GPI-anchor) also, and whether their GPI-like-anchor
CC       modifications can be interconverted with GPI-anchor modifications in a
CC       resculpting process. It has not been established that the GPI-like-
CC       anchor modification in Dictyosteliida utilizes the same sequence motif.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAFI02000119; EAL63128.1; -; Genomic_DNA.
DR   RefSeq; XP_636631.1; XM_631539.1.
DR   AlphaFoldDB; Q54IM7; -.
DR   PaxDb; Q54IM7; -.
DR   EnsemblProtists; EAL63128; EAL63128; DDB_G0288649.
DR   GeneID; 8626732; -.
DR   KEGG; ddi:DDB_G0288649; -.
DR   dictyBase; DDB_G0288649; ponL.
DR   HOGENOM; CLU_1800080_0_0_1; -.
DR   PRO; PR:Q54IM7; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Actin-binding; Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein;
KW   Membrane; Reference proteome; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..119
FT                   /note="Ponticulin-like protein L"
FT                   /id="PRO_0000367839"
FT   PROPEP          120..144
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000367840"
FT   LIPID           119
FT                   /note="GPI-like-anchor amidated serine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        48
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        74
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        90
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        103
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        116
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   144 AA;  16081 MW;  55ED9BFB35580E26 CRC64;
     MKLTLLLIIS FILFSNVVFS QKFNLFNFEQ NHCLTTPELF LDECQLLNST LPMCKGYMRV
     TYVSSNTYEF SIFNTSTPLC GQVNRKAGSN FTCDQSTNGV KQNYTGFSVT CISTSNESSL
     SSKTQQSIVF TVLLLSLALI ASIF