POP1_ARATH
ID POP1_ARATH Reviewed; 826 AA.
AC F4IL30; F4IL31; L0N807; O22903; O22904; Q4PL83; Q4PL84;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Ribonucleases P/MRP protein subunit POP1 {ECO:0000303|PubMed:22549728, ECO:0000303|PubMed:22641852};
DE Short=Pop1p {ECO:0000303|PubMed:22641852};
DE Short=RNase P/MRP POP1 {ECO:0000303|PubMed:22549728, ECO:0000303|PubMed:22641852};
DE AltName: Full=RNA-processing protein POP1 {ECO:0000303|PubMed:22549728};
GN Name=POP1 {ECO:0000303|PubMed:22549728};
GN OrderedLocusNames=At2g47300 {ECO:0000312|Araport:AT2G47300};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, AND SUBUNIT.
RC STRAIN=cv. Columbia; TISSUE=Leaf;
RX PubMed=22641852; DOI=10.1093/nar/gks476;
RA Krehan M., Heubeck C., Menzel N., Seibel P., Schoen A.;
RT "RNase MRP RNA and RNase P activity in plants are associated with a Pop1p
RT containing complex.";
RL Nucleic Acids Res. 40:7956-7966(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=16244158; DOI=10.1104/pp.105.063479;
RA Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L.,
RA Ayele M., Haas B.J., Wu H.C., Town C.D.;
RT "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2
RT reveals numerous transcript variants.";
RL Plant Physiol. 139:1323-1337(2005).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22549728; DOI=10.1101/gad.189514.112;
RA Gutmann B., Gobert A., Giege P.;
RT "PRORP proteins support RNase P activity in both organelles and the nucleus
RT in Arabidopsis.";
RL Genes Dev. 26:1022-1027(2012).
CC -!- FUNCTION: Component of ribonuclease P, a ribonucleoprotein complex that
CC generates mature tRNA molecules by cleaving their 5'-ends
CC (PubMed:22641852). Also a component of the MRP ribonuclease complex,
CC which cleaves pre-rRNA sequences (PubMed:22549728). Required for rRNA
CC maturation, including 5.8S rRNA processing (PubMed:22549728).
CC {ECO:0000269|PubMed:22549728, ECO:0000269|PubMed:22641852}.
CC -!- SUBUNIT: Component of nuclear RNase P and RNase MRP ribonucleoproteins
CC (PubMed:22641852). RNase P consists of a catalytic RNA moiety and
CC different protein chains (By similarity). Several subunits of RNase P
CC are also part of the RNase MRP complex (By similarity). RNase MRP
CC consists of a catalytic RNA moiety and several protein subunits (By
CC similarity). {ECO:0000250|UniProtKB:Q99575,
CC ECO:0000269|PubMed:22641852}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC Nucleus, nucleolus {ECO:0000250|UniProtKB:Q99575}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1; Synonyms=Var 2a {ECO:0000303|PubMed:22641852};
CC IsoId=F4IL30-1; Sequence=Displayed;
CC Name=2 {ECO:0000312|EMBL:AAY82264.1, ECO:0000312|EMBL:AEC10825.1};
CC Synonyms=Var 2b {ECO:0000303|PubMed:22641852};
CC IsoId=F4IL30-2; Sequence=VSP_061002;
CC Name=3; Synonyms=Var 3 {ECO:0000303|PubMed:22641852};
CC IsoId=F4IL30-3; Sequence=VSP_061005, VSP_061006;
CC Name=4; Synonyms=Var 1 {ECO:0000303|PubMed:22641852};
CC IsoId=F4IL30-4; Sequence=VSP_061003, VSP_061004;
CC -!- DISRUPTION PHENOTYPE: Accumulation of unprocessed cytosolic rRNA
CC precursors, especially the large 35S rRNA precursor, as well as of the
CC internal transcribed spacer 1 (ITS1) fragment corresponding to the
CC region between the 18S and 5.8S rRNAs that contains the A3 cleavage
CC sites. {ECO:0000269|PubMed:22549728}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB63830.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; FN673552; CBK00919.1; -; mRNA.
DR EMBL; AC002337; AAB63829.1; -; Genomic_DNA.
DR EMBL; AC002337; AAB63830.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10824.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10825.1; -; Genomic_DNA.
DR EMBL; DQ069804; AAY82263.1; -; mRNA.
DR EMBL; DQ069805; AAY82264.1; -; mRNA.
DR PIR; D84913; D84913.
DR PIR; E84913; E84913.
DR RefSeq; NP_001078072.1; NM_001084603.2. [F4IL30-1]
DR RefSeq; NP_001078073.1; NM_001084604.1. [F4IL30-2]
DR AlphaFoldDB; F4IL30; -.
DR SMR; F4IL30; -.
DR STRING; 3702.AT2G47300.2; -.
DR PaxDb; F4IL30; -.
DR PRIDE; F4IL30; -.
DR ProteomicsDB; 212360; -.
DR ProteomicsDB; 252229; -.
DR EnsemblPlants; AT2G47300.2; AT2G47300.2; AT2G47300. [F4IL30-1]
DR EnsemblPlants; AT2G47300.3; AT2G47300.3; AT2G47300. [F4IL30-2]
DR GeneID; 819343; -.
DR Gramene; AT2G47300.2; AT2G47300.2; AT2G47300. [F4IL30-1]
DR Gramene; AT2G47300.3; AT2G47300.3; AT2G47300. [F4IL30-2]
DR KEGG; ath:AT2G47300; -.
DR Araport; AT2G47300; -.
DR TAIR; locus:2065175; AT2G47300.
DR eggNOG; KOG3322; Eukaryota.
DR HOGENOM; CLU_018268_0_0_1; -.
DR InParanoid; F4IL30; -.
DR OrthoDB; 543991at2759; -.
DR BRENDA; 3.1.26.5; 399.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IL30; baseline and differential.
DR GO; GO:0005655; C:nucleolar ribonuclease P complex; IBA:GO_Central.
DR GO; GO:0000172; C:ribonuclease MRP complex; IBA:GO_Central.
DR GO; GO:0004526; F:ribonuclease P activity; IEA:UniProtKB-EC.
DR GO; GO:0090502; P:RNA phosphodiester bond hydrolysis, endonucleolytic; IBA:GO_Central.
DR GO; GO:0006364; P:rRNA processing; IMP:TAIR.
DR InterPro; IPR039182; Pop1.
DR InterPro; IPR009723; Pop1_N.
DR InterPro; IPR012590; POPLD_dom.
DR PANTHER; PTHR22731; PTHR22731; 1.
DR Pfam; PF06978; POP1; 1.
DR Pfam; PF08170; POPLD; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome; rRNA processing;
KW tRNA processing.
FT CHAIN 1..826
FT /note="Ribonucleases P/MRP protein subunit POP1"
FT /id="PRO_0000452458"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 49..91
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 58..65
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 53..78
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..183
FT /note="MATTANGNSKKRDGGLSSLAPRKINVQKFSEARAPELESLHSIVSERLNKDF
FT RSKRNKRRRTNSYNNQPAKKRNIKRQKSQSLIGQVSGGDHEVKITRRVKRRMELKGNPE
FT TGFCTSGDGTKRLRTHVWHAKRFTMTKLWGFHLPLGLHGRGRGSRDVLKQSRQGVLVHD
FT ASYHIAVQLEGPE -> MMQAITLLCNWRVQRQ (in isoform 2)"
FT /id="VSP_061002"
FT VAR_SEQ 184..190
FT /note="GSLLSIL -> AGIHTYS (in isoform 4)"
FT /id="VSP_061003"
FT VAR_SEQ 191..826
FT /note="Missing (in isoform 4)"
FT /id="VSP_061004"
FT VAR_SEQ 297..307
FT /note="MNETGVSVDCF -> VAICCLLRLFL (in isoform 3)"
FT /id="VSP_061005"
FT VAR_SEQ 308..826
FT /note="Missing (in isoform 3)"
FT /id="VSP_061006"
FT CONFLICT 674
FT /note="L -> F (in Ref. 4; AAY82263/AAY82264)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 826 AA; 92565 MW; 2253210B41373E03 CRC64;
MATTANGNSK KRDGGLSSLA PRKINVQKFS EARAPELESL HSIVSERLNK DFRSKRNKRR
RTNSYNNQPA KKRNIKRQKS QSLIGQVSGG DHEVKITRRV KRRMELKGNP ETGFCTSGDG
TKRLRTHVWH AKRFTMTKLW GFHLPLGLHG RGRGSRDVLK QSRQGVLVHD ASYHIAVQLE
GPEGSLLSIL NMLLEPSPSS HSKEVFDSIL TGGSYENAML YHVEPPVSQA IAPVTYMWRP
SKIPKRRNEE KGGDGIGTDL PVSDKDHEDF RKLWVWIHAS SFSEGYAILK VACQKQMNET
GVSVDCFSLE GQLAKLEIFG SKASHLLQKT LHPATSTSEN PSILRKCSME KAEVKNVADL
YTEENVSSGA ILAQFVIDPR LILTSPHDDR TVSVETIKTE PTESVETTTN TEAETFPEVF
NCLWDANSEL TPPEEENMLC WEKHQSRMDS LCLDDPAAEV PKVSSRPRSS RSCPLLLLKH
KKLGNAPTGW SLILPLSWIK VFWNAFVSKG AHAIGQREKR WVSCDDGLPF FPSDFPDCKA
YSSFTLSEAA DLEEKAQRRP PAIRPFRIPI PPPWNSIHVT RSIGEGSNQK FSSNGRSVVE
ISSYGGNLFD GIVARTSDSL TTFLQTFTSD NMLLFPHNTS KPSTDLMMTL QEDDKKVRAQ
IHQSSNKLCL VRVLLHAFKE GSFEEGAVVC APTLADISLL KSSCSEGEDG RVTIPQSSVS
SYFQEQPCGT WELNVPEDTL TEQSHRWPIG FVTTGFVRGS KKPAAEAFCD AVLLGRLRDE
QWRDKDVRRR KKQIYVLVRN LRSSAFRLAL ATIVLEQQDY CDVHCF
