POP4_ARATH
ID POP4_ARATH Reviewed; 296 AA.
AC Q9ZW76; A0A178W358; F4IQ79;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Ribonuclease MRP protein subunit POP4 {ECO:0000303|PubMed:22549728};
DE Short=RNase MRP POP4 {ECO:0000303|PubMed:22549728};
DE AltName: Full=RNA-processing protein POP4 {ECO:0000303|PubMed:22549728};
GN Name=POP4 {ECO:0000303|PubMed:22549728};
GN OrderedLocusNames=At2g43190 {ECO:0000312|Araport:AT2G43190};
GN ORFNames=F14B2.13 {ECO:0000312|EMBL:AAC64308.2};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22549728; DOI=10.1101/gad.189514.112;
RA Gutmann B., Gobert A., Giege P.;
RT "PRORP proteins support RNase P activity in both organelles and the nucleus
RT in Arabidopsis.";
RL Genes Dev. 26:1022-1027(2012).
CC -!- FUNCTION: Component of the MRP ribonuclease complex, which cleaves pre-
CC rRNA sequences (PubMed:22549728). Required for rRNA maturation,
CC including 5.8S rRNA processing (PubMed:22549728). Seems not involved in
CC tRNA maturation (PubMed:22549728). {ECO:0000269|PubMed:22549728}.
CC -!- SUBUNIT: Component of nuclear RNase MRP complexes (By similarity).
CC Several subunits of RNase P are also part of the RNase MRP complex (By
CC similarity). RNase MRP consists of a catalytic RNA moiety and several
CC protein subunits (By similarity). {ECO:0000250|UniProtKB:P38336,
CC ECO:0000250|UniProtKB:Q99575}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9ZW76-1; Sequence=Displayed;
CC Name=2 {ECO:0000312|EMBL:AEC10225.1, ECO:0000312|EMBL:AEC10226.1};
CC IsoId=Q9ZW76-2; Sequence=VSP_061007;
CC -!- DISRUPTION PHENOTYPE: Accumulation of unprocessed cytosolic rRNA
CC precursors, especially the large 35S rRNA precursor, as well as of the
CC internal transcribed spacer 1 (ITS1) fragment corresponding to the
CC region between the 18S and 5.8S rRNAs that contains the A3 cleavage
CC sites. {ECO:0000269|PubMed:22549728}.
CC -!- SIMILARITY: Belongs to the eukaryotic/archaeal RNase P protein
CC component 1 family. {ECO:0000305}.
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DR EMBL; AC004450; AAC64308.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC10224.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10225.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10226.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62650.1; -; Genomic_DNA.
DR EMBL; AY086048; AAM63258.1; -; mRNA.
DR PIR; B84863; B84863.
DR RefSeq; NP_001324792.1; NM_001337019.1. [Q9ZW76-1]
DR RefSeq; NP_565993.1; NM_129881.3. [Q9ZW76-1]
DR RefSeq; NP_850389.1; NM_180058.2. [Q9ZW76-2]
DR RefSeq; NP_973678.1; NM_201949.2. [Q9ZW76-2]
DR AlphaFoldDB; Q9ZW76; -.
DR SMR; Q9ZW76; -.
DR STRING; 3702.AT2G43190.1; -.
DR PaxDb; Q9ZW76; -.
DR PRIDE; Q9ZW76; -.
DR ProteomicsDB; 183689; -.
DR ProteomicsDB; 189221; -.
DR EnsemblPlants; AT2G43190.1; AT2G43190.1; AT2G43190. [Q9ZW76-1]
DR EnsemblPlants; AT2G43190.2; AT2G43190.2; AT2G43190. [Q9ZW76-2]
DR EnsemblPlants; AT2G43190.3; AT2G43190.3; AT2G43190. [Q9ZW76-2]
DR EnsemblPlants; AT2G43190.5; AT2G43190.5; AT2G43190. [Q9ZW76-1]
DR GeneID; 818920; -.
DR Gramene; AT2G43190.1; AT2G43190.1; AT2G43190. [Q9ZW76-1]
DR Gramene; AT2G43190.2; AT2G43190.2; AT2G43190. [Q9ZW76-2]
DR Gramene; AT2G43190.3; AT2G43190.3; AT2G43190. [Q9ZW76-2]
DR Gramene; AT2G43190.5; AT2G43190.5; AT2G43190. [Q9ZW76-1]
DR KEGG; ath:AT2G43190; -.
DR Araport; AT2G43190; -.
DR TAIR; locus:2041021; AT2G43190.
DR eggNOG; KOG4046; Eukaryota.
DR HOGENOM; CLU_049812_0_0_1; -.
DR InParanoid; Q9ZW76; -.
DR OMA; KPRCQTR; -.
DR OrthoDB; 1362700at2759; -.
DR PhylomeDB; Q9ZW76; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZW76; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000172; C:ribonuclease MRP complex; IBA:GO_Central.
DR GO; GO:0030677; C:ribonuclease P complex; IBA:GO_Central.
DR GO; GO:0033204; F:ribonuclease P RNA binding; IBA:GO_Central.
DR GO; GO:0006364; P:rRNA processing; IMP:TAIR.
DR Gene3D; 2.30.30.210; -; 1.
DR InterPro; IPR016848; RNase_P/MRP_Rpp29-subunit.
DR InterPro; IPR036980; RNase_P/MRP_Rpp29_sf.
DR InterPro; IPR023534; Rof/RNase_P-like.
DR InterPro; IPR002730; Rpp29/RNP1.
DR PANTHER; PTHR13348; PTHR13348; 1.
DR Pfam; PF01868; RNase_P-MRP_p29; 1.
DR SMART; SM00538; POP4; 1.
DR SUPFAM; SSF101744; SSF101744; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Nucleus; Reference proteome; rRNA processing.
FT CHAIN 1..296
FT /note="Ribonuclease MRP protein subunit POP4"
FT /id="PRO_0000452459"
FT REGION 29..74
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 148..173
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 160..167
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 35..53
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 71
FT /note="Missing (in isoform 2)"
FT /id="VSP_061007"
SQ SEQUENCE 296 AA; 33266 MW; F7D25DCB95CEFEA8 CRC64;
MGTETVVHDQ ARWAMAAMER RLAVAKAQLL QQQQQKNEKD KKGTSDVDVS MKESHQADSL
PTPSKTSIKK VDPKDDDSVA YTKLSHPVDE NLLATNVKFS SAKGTIVDKV LHNLLRSGDS
AQKYLQGTKS VKLDNYILLD NFVQSRSSAS GSKKASQKDS KRSKSRMSMK RLKKSGALHI
PKDLQKFDLF KPMHGMWESY MMKLIKVTGK IQLSLTLLSA DLHGAFMFVA ECKIASFTGV
QGIMVRETSE TFGIITRDDK FRVVPKKLSV FIIQLDCWKI TLHGDKFISR DNIVQR
